Combinatorial microRNA Loading into Extracellular Vesicles for Increased Anti-Inflammatory Efficacy
Extracellular vesicles (EVs) have emerged as promising therapeutic entities in part due to their potential to regulate multiple signaling pathways in target cells. This potential is derived from the broad array of constituent and/or cargo molecules associated with EVs. Among these, microRNAs (miRNAs...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-10-01
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| Series: | Non-Coding RNA |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2311-553X/8/5/71 |
| _version_ | 1797470721040973824 |
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| author | Alex Eli Pottash Daniel Levy Anjana Jeyaram Leo Kuo Stephanie M. Kronstadt Wei Chao Steven M. Jay |
| author_facet | Alex Eli Pottash Daniel Levy Anjana Jeyaram Leo Kuo Stephanie M. Kronstadt Wei Chao Steven M. Jay |
| author_sort | Alex Eli Pottash |
| collection | DOAJ |
| description | Extracellular vesicles (EVs) have emerged as promising therapeutic entities in part due to their potential to regulate multiple signaling pathways in target cells. This potential is derived from the broad array of constituent and/or cargo molecules associated with EVs. Among these, microRNAs (miRNAs) are commonly implicated as important and have been associated with a wide variety of EV-induced biological phenomena. While controlled loading of single miRNAs is a well-documented approach for enhancing EV bioactivity, loading of multiple miRNAs has not been fully leveraged to maximize the potential of EV-based therapies. Here, an established approach to extrinsic nucleic acid loading of EVs, sonication, was utilized to load multiple miRNAs in HEK293T EVs. Combinations of miRNAs were compared to single miRNAs with respect to anti-inflammatory outcomes in assays of increasing stringency, with the combination of miR-146a, miR-155, and miR-223 found to have the most potential amongst the tested groups. |
| first_indexed | 2024-03-09T19:40:02Z |
| format | Article |
| id | doaj.art-ffb4c65ba4504a89a24698d30b281c3e |
| institution | Directory Open Access Journal |
| issn | 2311-553X |
| language | English |
| last_indexed | 2024-03-09T19:40:02Z |
| publishDate | 2022-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Non-Coding RNA |
| spelling | doaj.art-ffb4c65ba4504a89a24698d30b281c3e2023-11-24T01:42:33ZengMDPI AGNon-Coding RNA2311-553X2022-10-01857110.3390/ncrna8050071Combinatorial microRNA Loading into Extracellular Vesicles for Increased Anti-Inflammatory EfficacyAlex Eli Pottash0Daniel Levy1Anjana Jeyaram2Leo Kuo3Stephanie M. Kronstadt4Wei Chao5Steven M. Jay6Fischell Department of Bioengineering, University of Maryland, 8278 Paint Branch Drive, College Park, MD 20742, USAFischell Department of Bioengineering, University of Maryland, 8278 Paint Branch Drive, College Park, MD 20742, USAFischell Department of Bioengineering, University of Maryland, 8278 Paint Branch Drive, College Park, MD 20742, USAFischell Department of Bioengineering, University of Maryland, 8278 Paint Branch Drive, College Park, MD 20742, USAFischell Department of Bioengineering, University of Maryland, 8278 Paint Branch Drive, College Park, MD 20742, USATranslational Research Program, Department of Anesthesiology and Center for Shock, Trauma and Anesthesiology Research, University of Maryland School of Medicine, 660 West Redwood Street, Baltimore, MD 21201, USAFischell Department of Bioengineering, University of Maryland, 8278 Paint Branch Drive, College Park, MD 20742, USAExtracellular vesicles (EVs) have emerged as promising therapeutic entities in part due to their potential to regulate multiple signaling pathways in target cells. This potential is derived from the broad array of constituent and/or cargo molecules associated with EVs. Among these, microRNAs (miRNAs) are commonly implicated as important and have been associated with a wide variety of EV-induced biological phenomena. While controlled loading of single miRNAs is a well-documented approach for enhancing EV bioactivity, loading of multiple miRNAs has not been fully leveraged to maximize the potential of EV-based therapies. Here, an established approach to extrinsic nucleic acid loading of EVs, sonication, was utilized to load multiple miRNAs in HEK293T EVs. Combinations of miRNAs were compared to single miRNAs with respect to anti-inflammatory outcomes in assays of increasing stringency, with the combination of miR-146a, miR-155, and miR-223 found to have the most potential amongst the tested groups.https://www.mdpi.com/2311-553X/8/5/71exosomesmiRNAinflammationsepsis |
| spellingShingle | Alex Eli Pottash Daniel Levy Anjana Jeyaram Leo Kuo Stephanie M. Kronstadt Wei Chao Steven M. Jay Combinatorial microRNA Loading into Extracellular Vesicles for Increased Anti-Inflammatory Efficacy Non-Coding RNA exosomes miRNA inflammation sepsis |
| title | Combinatorial microRNA Loading into Extracellular Vesicles for Increased Anti-Inflammatory Efficacy |
| title_full | Combinatorial microRNA Loading into Extracellular Vesicles for Increased Anti-Inflammatory Efficacy |
| title_fullStr | Combinatorial microRNA Loading into Extracellular Vesicles for Increased Anti-Inflammatory Efficacy |
| title_full_unstemmed | Combinatorial microRNA Loading into Extracellular Vesicles for Increased Anti-Inflammatory Efficacy |
| title_short | Combinatorial microRNA Loading into Extracellular Vesicles for Increased Anti-Inflammatory Efficacy |
| title_sort | combinatorial microrna loading into extracellular vesicles for increased anti inflammatory efficacy |
| topic | exosomes miRNA inflammation sepsis |
| url | https://www.mdpi.com/2311-553X/8/5/71 |
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