Evaluation of Prebiotic Potential of Crude Polysaccharides Extracted from Wild <i>Lentinus polychrous</i> and <i>Lentinus squarrosulus</i> and Their Application for a Formulation of a Novel Lyophilized Synbiotic
Edible mushrooms, including wild mushrooms, are currently being investigated as natural sources to evaluate their prebiotic potential. This study aimed to evaluate the prebiotic potential of crude polysaccharides (CPSs) extracted from wild <i>Lentinus squarrosulus</i> UBU_LS1 and <i&g...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2024-01-01
|
| Series: | Foods |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2304-8158/13/2/287 |
| _version_ | 1827369644821839872 |
|---|---|
| author | Marutpong Panya Chamraj Kaewraemruaen Phairo Saenwang Patcharin Pimboon |
| author_facet | Marutpong Panya Chamraj Kaewraemruaen Phairo Saenwang Patcharin Pimboon |
| author_sort | Marutpong Panya |
| collection | DOAJ |
| description | Edible mushrooms, including wild mushrooms, are currently being investigated as natural sources to evaluate their prebiotic potential. This study aimed to evaluate the prebiotic potential of crude polysaccharides (CPSs) extracted from wild <i>Lentinus squarrosulus</i> UBU_LS1 and <i>Lentinus polychrous</i> UBU_LP2 and their application as cryoprotectants in the freeze-drying process to formulate a novel synbiotic product. Based on fruiting body morphology and molecular identification, two wild edible mushrooms named UBU_LS1 and UBU_LP2 were identified as <i>Lentinus squarrosulus</i> and <i>Lentinus polychrous,</i> respectively. <i>L. squarrosulus</i> UBU_LS1 and <i>L. polychrous</i> UBU_LP2 contained high amounts of CPS after hot water extraction. Monosaccharide component analysis showed that CPS_UBU_LS1 and CPS_UBU_LP2 were typical heteropolysaccharides. CPS_UBU_LS1 and CPS_UBU_LP2 showed hydrolysis tolerance to the simulated human gastric acidic pH solution, indicating that these CPSs are capable of reaching the lower gastrointestinal tract. Antioxidant activity determined using the 1,1-diphenyl-2-picrylhydrazyl assay revealed that the CPS_UBU_LS1 and CPS_UBU_LP2 displayed greater antioxidant activity comparable with that of ascorbic acid. It was found that CPS_UBU_LS1 and CPS_UBU_LP2 have a high potential for stimulating growth in all probiotic strains. Moreover, both CPS compounds could possibly be used as cryoprotectants in freeze drying, since the viability of the selected probiotic <i>L. fermentum</i> 47-7 exhibited cell survival of greater than 70% after 90 days of storage at 4 °C. These results highlight that wild edible mushrooms <i>L. squarrosulus</i> UBU_LS1 and <i>L. polychrous</i> UBU_LP2 are potential natural sources of prebiotics and can be applied as cryoprotectants in the freeze-drying process. The crude polysaccharide derived from this study could also be considered as a potent antioxidative compound. Therefore, our study provides evidence to support the application of CPSs from wild edible mushrooms in synbiotic product development and in various functional foods. Finally, further evaluation of these prebiotics, including the determination of the potential rehabilitation of beneficial gut microbes in diseased individuals, is currently being conducted by our research group. |
| first_indexed | 2024-03-08T09:55:28Z |
| format | Article |
| id | doaj.art-ffb5bf2b823a4098945d9501c59d2f6d |
| institution | Directory Open Access Journal |
| issn | 2304-8158 |
| language | English |
| last_indexed | 2024-03-08T09:55:28Z |
| publishDate | 2024-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Foods |
| spelling | doaj.art-ffb5bf2b823a4098945d9501c59d2f6d2024-01-29T13:52:27ZengMDPI AGFoods2304-81582024-01-0113228710.3390/foods13020287Evaluation of Prebiotic Potential of Crude Polysaccharides Extracted from Wild <i>Lentinus polychrous</i> and <i>Lentinus squarrosulus</i> and Their Application for a Formulation of a Novel Lyophilized SynbioticMarutpong Panya0Chamraj Kaewraemruaen1Phairo Saenwang2Patcharin Pimboon3Research Group for Biomedical Research and Innovative Development (RG-BRID), College of Medicine and Public Health, Ubon Ratchathani University, Warinchamrap, Ubon Ratchathani 34190, ThailandDepartment of Science and Bioinnovation, Faculty of Liberal Arts and Science, Kasetsart University, Kamphaeng Saen Campus, Nakhon Pathom 73140, ThailandResearch Group for Biomedical Research and Innovative Development (RG-BRID), College of Medicine and Public Health, Ubon Ratchathani University, Warinchamrap, Ubon Ratchathani 34190, ThailandCollege of Medicine and Public Health, Ubon Ratchathani University, Warinchamrap, Ubon Ratchathani 34190, ThailandEdible mushrooms, including wild mushrooms, are currently being investigated as natural sources to evaluate their prebiotic potential. This study aimed to evaluate the prebiotic potential of crude polysaccharides (CPSs) extracted from wild <i>Lentinus squarrosulus</i> UBU_LS1 and <i>Lentinus polychrous</i> UBU_LP2 and their application as cryoprotectants in the freeze-drying process to formulate a novel synbiotic product. Based on fruiting body morphology and molecular identification, two wild edible mushrooms named UBU_LS1 and UBU_LP2 were identified as <i>Lentinus squarrosulus</i> and <i>Lentinus polychrous,</i> respectively. <i>L. squarrosulus</i> UBU_LS1 and <i>L. polychrous</i> UBU_LP2 contained high amounts of CPS after hot water extraction. Monosaccharide component analysis showed that CPS_UBU_LS1 and CPS_UBU_LP2 were typical heteropolysaccharides. CPS_UBU_LS1 and CPS_UBU_LP2 showed hydrolysis tolerance to the simulated human gastric acidic pH solution, indicating that these CPSs are capable of reaching the lower gastrointestinal tract. Antioxidant activity determined using the 1,1-diphenyl-2-picrylhydrazyl assay revealed that the CPS_UBU_LS1 and CPS_UBU_LP2 displayed greater antioxidant activity comparable with that of ascorbic acid. It was found that CPS_UBU_LS1 and CPS_UBU_LP2 have a high potential for stimulating growth in all probiotic strains. Moreover, both CPS compounds could possibly be used as cryoprotectants in freeze drying, since the viability of the selected probiotic <i>L. fermentum</i> 47-7 exhibited cell survival of greater than 70% after 90 days of storage at 4 °C. These results highlight that wild edible mushrooms <i>L. squarrosulus</i> UBU_LS1 and <i>L. polychrous</i> UBU_LP2 are potential natural sources of prebiotics and can be applied as cryoprotectants in the freeze-drying process. The crude polysaccharide derived from this study could also be considered as a potent antioxidative compound. Therefore, our study provides evidence to support the application of CPSs from wild edible mushrooms in synbiotic product development and in various functional foods. Finally, further evaluation of these prebiotics, including the determination of the potential rehabilitation of beneficial gut microbes in diseased individuals, is currently being conducted by our research group.https://www.mdpi.com/2304-8158/13/2/287polysaccharideprobioticprebioticsynbiotic<i>Lentinus polychrous</i><i>Lentinus squarrosulus</i> |
| spellingShingle | Marutpong Panya Chamraj Kaewraemruaen Phairo Saenwang Patcharin Pimboon Evaluation of Prebiotic Potential of Crude Polysaccharides Extracted from Wild <i>Lentinus polychrous</i> and <i>Lentinus squarrosulus</i> and Their Application for a Formulation of a Novel Lyophilized Synbiotic Foods polysaccharide probiotic prebiotic synbiotic <i>Lentinus polychrous</i> <i>Lentinus squarrosulus</i> |
| title | Evaluation of Prebiotic Potential of Crude Polysaccharides Extracted from Wild <i>Lentinus polychrous</i> and <i>Lentinus squarrosulus</i> and Their Application for a Formulation of a Novel Lyophilized Synbiotic |
| title_full | Evaluation of Prebiotic Potential of Crude Polysaccharides Extracted from Wild <i>Lentinus polychrous</i> and <i>Lentinus squarrosulus</i> and Their Application for a Formulation of a Novel Lyophilized Synbiotic |
| title_fullStr | Evaluation of Prebiotic Potential of Crude Polysaccharides Extracted from Wild <i>Lentinus polychrous</i> and <i>Lentinus squarrosulus</i> and Their Application for a Formulation of a Novel Lyophilized Synbiotic |
| title_full_unstemmed | Evaluation of Prebiotic Potential of Crude Polysaccharides Extracted from Wild <i>Lentinus polychrous</i> and <i>Lentinus squarrosulus</i> and Their Application for a Formulation of a Novel Lyophilized Synbiotic |
| title_short | Evaluation of Prebiotic Potential of Crude Polysaccharides Extracted from Wild <i>Lentinus polychrous</i> and <i>Lentinus squarrosulus</i> and Their Application for a Formulation of a Novel Lyophilized Synbiotic |
| title_sort | evaluation of prebiotic potential of crude polysaccharides extracted from wild i lentinus polychrous i and i lentinus squarrosulus i and their application for a formulation of a novel lyophilized synbiotic |
| topic | polysaccharide probiotic prebiotic synbiotic <i>Lentinus polychrous</i> <i>Lentinus squarrosulus</i> |
| url | https://www.mdpi.com/2304-8158/13/2/287 |
| work_keys_str_mv | AT marutpongpanya evaluationofprebioticpotentialofcrudepolysaccharidesextractedfromwildilentinuspolychrousiandilentinussquarrosulusiandtheirapplicationforaformulationofanovellyophilizedsynbiotic AT chamrajkaewraemruaen evaluationofprebioticpotentialofcrudepolysaccharidesextractedfromwildilentinuspolychrousiandilentinussquarrosulusiandtheirapplicationforaformulationofanovellyophilizedsynbiotic AT phairosaenwang evaluationofprebioticpotentialofcrudepolysaccharidesextractedfromwildilentinuspolychrousiandilentinussquarrosulusiandtheirapplicationforaformulationofanovellyophilizedsynbiotic AT patcharinpimboon evaluationofprebioticpotentialofcrudepolysaccharidesextractedfromwildilentinuspolychrousiandilentinussquarrosulusiandtheirapplicationforaformulationofanovellyophilizedsynbiotic |