Effects of Oleacein on High-Fat Diet-Dependent Steatosis, Weight Gain, and Insulin Resistance in Mice
Many reports indicate that the protective action of nutraceuticals in the Mediterranean diet, against metabolic and cardiovascular diseases, can be attributed to the action of polyphenolic components of extra-virgin olive oil (EVOO). Here, we evaluated the protective effects of oleacein, one of the...
| Main Authors: | , , , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2018-03-01
|
| Series: | Frontiers in Endocrinology |
| Subjects: | |
| Online Access: | http://journal.frontiersin.org/article/10.3389/fendo.2018.00116/full |
| _version_ | 1828525970418565120 |
|---|---|
| author | Giovanni Enrico Lombardo Saverio Massimo Lepore Valeria Maria Morittu Biagio Arcidiacono Carmela Colica Antonio Procopio Valentina Maggisano Stefania Bulotta Nicola Costa Chiara Mignogna Chiara Mignogna Domenico Britti Antonio Brunetti Diego Russo Marilena Celano |
| author_facet | Giovanni Enrico Lombardo Saverio Massimo Lepore Valeria Maria Morittu Biagio Arcidiacono Carmela Colica Antonio Procopio Valentina Maggisano Stefania Bulotta Nicola Costa Chiara Mignogna Chiara Mignogna Domenico Britti Antonio Brunetti Diego Russo Marilena Celano |
| author_sort | Giovanni Enrico Lombardo |
| collection | DOAJ |
| description | Many reports indicate that the protective action of nutraceuticals in the Mediterranean diet, against metabolic and cardiovascular diseases, can be attributed to the action of polyphenolic components of extra-virgin olive oil (EVOO). Here, we evaluated the protective effects of oleacein, one of the most abundant secoiridoids in EVOO, on the damages/metabolic alterations caused by high-fat diet (HFD) in male C57BL/6JolaHsd mice. After 5 weeks of treatment with 20 mg/kg of oleacein, body weight, glycemia, insulinemia, serum lipids, and histologic examination of liver tissue indicated a protective action of oleacein against abdominal fat accumulation, weight gain, and liver steatosis, with improvement of insulin-dependent glucose and lipid metabolism. Both serum parameters and hepatic histologic examination were altered in mice fed with HFD. By contrast, in the animals that received oleacein, plasma glucose, cholesterol and triglyceride serum levels, and liver histology were similar to controls fed with normocaloric diet. In addition, protein levels of FAS, SREBP-1, and phospho-ERK in liver were positively modulated by oleacein, indicating an improvement in liver insulin sensitivity. In a group of obese mice, treatment with oleacein determined a light, but still significant reduction of the increase in body weight, mainly due to lesser liver steatosis enlargement, associated with reduced levels of SREBP-1 and phospho-ERK and lower levels of total serum cholesterol; in these animals, altered plasma glucose and triglyceride serum levels were not reverted by oleacein. These results indicate that HFD-related hepatic insulin resistance may be partially prevented by oral administration of oleacein, suggesting a protective role of this nutraceutical against diet-dependent metabolic alterations. Additional studies are necessary to check whether oleacein can be used as an adjuvant to improve insulin sensitivity in humans. |
| first_indexed | 2024-12-11T21:16:21Z |
| format | Article |
| id | doaj.art-ffb860feee0046f3b7ff6af25801d946 |
| institution | Directory Open Access Journal |
| issn | 1664-2392 |
| language | English |
| last_indexed | 2024-12-11T21:16:21Z |
| publishDate | 2018-03-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Endocrinology |
| spelling | doaj.art-ffb860feee0046f3b7ff6af25801d9462022-12-22T00:50:35ZengFrontiers Media S.A.Frontiers in Endocrinology1664-23922018-03-01910.3389/fendo.2018.00116347511Effects of Oleacein on High-Fat Diet-Dependent Steatosis, Weight Gain, and Insulin Resistance in MiceGiovanni Enrico Lombardo0Saverio Massimo Lepore1Valeria Maria Morittu2Biagio Arcidiacono3Carmela Colica4Antonio Procopio5Valentina Maggisano6Stefania Bulotta7Nicola Costa8Chiara Mignogna9Chiara Mignogna10Domenico Britti11Antonio Brunetti12Diego Russo13Marilena Celano14Department of Health Sciences, University “Magna Græcia” of Catanzaro, Catanzaro, ItalyDepartment of Health Sciences, University “Magna Græcia” of Catanzaro, Catanzaro, ItalyDepartment of Health Sciences, University “Magna Græcia” of Catanzaro, Catanzaro, ItalyDepartment of Health Sciences, University “Magna Græcia” of Catanzaro, Catanzaro, ItalyCNR, IBFM UOS of Germaneto, University “Magna Græcia” of Catanzaro, Catanzaro, ItalyDepartment of Health Sciences, University “Magna Græcia” of Catanzaro, Catanzaro, ItalyDepartment of Health Sciences, University “Magna Græcia” of Catanzaro, Catanzaro, ItalyDepartment of Health Sciences, University “Magna Græcia” of Catanzaro, Catanzaro, ItalyDepartment of Health Sciences, University “Magna Græcia” of Catanzaro, Catanzaro, ItalyDepartment of Health Sciences, University “Magna Græcia” of Catanzaro, Catanzaro, ItalyInterdepartmental Service Center, University “Magna Græcia” of Catanzaro, Catanzaro, ItalyDepartment of Health Sciences, University “Magna Græcia” of Catanzaro, Catanzaro, ItalyDepartment of Health Sciences, University “Magna Græcia” of Catanzaro, Catanzaro, ItalyDepartment of Health Sciences, University “Magna Græcia” of Catanzaro, Catanzaro, ItalyDepartment of Health Sciences, University “Magna Græcia” of Catanzaro, Catanzaro, ItalyMany reports indicate that the protective action of nutraceuticals in the Mediterranean diet, against metabolic and cardiovascular diseases, can be attributed to the action of polyphenolic components of extra-virgin olive oil (EVOO). Here, we evaluated the protective effects of oleacein, one of the most abundant secoiridoids in EVOO, on the damages/metabolic alterations caused by high-fat diet (HFD) in male C57BL/6JolaHsd mice. After 5 weeks of treatment with 20 mg/kg of oleacein, body weight, glycemia, insulinemia, serum lipids, and histologic examination of liver tissue indicated a protective action of oleacein against abdominal fat accumulation, weight gain, and liver steatosis, with improvement of insulin-dependent glucose and lipid metabolism. Both serum parameters and hepatic histologic examination were altered in mice fed with HFD. By contrast, in the animals that received oleacein, plasma glucose, cholesterol and triglyceride serum levels, and liver histology were similar to controls fed with normocaloric diet. In addition, protein levels of FAS, SREBP-1, and phospho-ERK in liver were positively modulated by oleacein, indicating an improvement in liver insulin sensitivity. In a group of obese mice, treatment with oleacein determined a light, but still significant reduction of the increase in body weight, mainly due to lesser liver steatosis enlargement, associated with reduced levels of SREBP-1 and phospho-ERK and lower levels of total serum cholesterol; in these animals, altered plasma glucose and triglyceride serum levels were not reverted by oleacein. These results indicate that HFD-related hepatic insulin resistance may be partially prevented by oral administration of oleacein, suggesting a protective role of this nutraceutical against diet-dependent metabolic alterations. Additional studies are necessary to check whether oleacein can be used as an adjuvant to improve insulin sensitivity in humans.http://journal.frontiersin.org/article/10.3389/fendo.2018.00116/fulloleaceininsulin resistanceobesityliver steatosischolesterol |
| spellingShingle | Giovanni Enrico Lombardo Saverio Massimo Lepore Valeria Maria Morittu Biagio Arcidiacono Carmela Colica Antonio Procopio Valentina Maggisano Stefania Bulotta Nicola Costa Chiara Mignogna Chiara Mignogna Domenico Britti Antonio Brunetti Diego Russo Marilena Celano Effects of Oleacein on High-Fat Diet-Dependent Steatosis, Weight Gain, and Insulin Resistance in Mice Frontiers in Endocrinology oleacein insulin resistance obesity liver steatosis cholesterol |
| title | Effects of Oleacein on High-Fat Diet-Dependent Steatosis, Weight Gain, and Insulin Resistance in Mice |
| title_full | Effects of Oleacein on High-Fat Diet-Dependent Steatosis, Weight Gain, and Insulin Resistance in Mice |
| title_fullStr | Effects of Oleacein on High-Fat Diet-Dependent Steatosis, Weight Gain, and Insulin Resistance in Mice |
| title_full_unstemmed | Effects of Oleacein on High-Fat Diet-Dependent Steatosis, Weight Gain, and Insulin Resistance in Mice |
| title_short | Effects of Oleacein on High-Fat Diet-Dependent Steatosis, Weight Gain, and Insulin Resistance in Mice |
| title_sort | effects of oleacein on high fat diet dependent steatosis weight gain and insulin resistance in mice |
| topic | oleacein insulin resistance obesity liver steatosis cholesterol |
| url | http://journal.frontiersin.org/article/10.3389/fendo.2018.00116/full |
| work_keys_str_mv | AT giovannienricolombardo effectsofoleaceinonhighfatdietdependentsteatosisweightgainandinsulinresistanceinmice AT saveriomassimolepore effectsofoleaceinonhighfatdietdependentsteatosisweightgainandinsulinresistanceinmice AT valeriamariamorittu effectsofoleaceinonhighfatdietdependentsteatosisweightgainandinsulinresistanceinmice AT biagioarcidiacono effectsofoleaceinonhighfatdietdependentsteatosisweightgainandinsulinresistanceinmice AT carmelacolica effectsofoleaceinonhighfatdietdependentsteatosisweightgainandinsulinresistanceinmice AT antonioprocopio effectsofoleaceinonhighfatdietdependentsteatosisweightgainandinsulinresistanceinmice AT valentinamaggisano effectsofoleaceinonhighfatdietdependentsteatosisweightgainandinsulinresistanceinmice AT stefaniabulotta effectsofoleaceinonhighfatdietdependentsteatosisweightgainandinsulinresistanceinmice AT nicolacosta effectsofoleaceinonhighfatdietdependentsteatosisweightgainandinsulinresistanceinmice AT chiaramignogna effectsofoleaceinonhighfatdietdependentsteatosisweightgainandinsulinresistanceinmice AT chiaramignogna effectsofoleaceinonhighfatdietdependentsteatosisweightgainandinsulinresistanceinmice AT domenicobritti effectsofoleaceinonhighfatdietdependentsteatosisweightgainandinsulinresistanceinmice AT antoniobrunetti effectsofoleaceinonhighfatdietdependentsteatosisweightgainandinsulinresistanceinmice AT diegorusso effectsofoleaceinonhighfatdietdependentsteatosisweightgainandinsulinresistanceinmice AT marilenacelano effectsofoleaceinonhighfatdietdependentsteatosisweightgainandinsulinresistanceinmice |