Application of Isolated Guinea Pig Ileum Method for Detection of Histamine Substances in Osteopeptide Injections
ObjectiveTo evaluate the detection of the histamine substances in osteopeptide injections by the isolated guinea pig ileum method, and to explore the feasibility of replacing cat depressor substances method.MethodsAccording to the 2020 edition of Chinese Pharmacopoeia, the histamine substances in 7...
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| Format: | Article |
| Language: | zho |
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Editorial Office of Laboratory Animal and Comparative Medicine
2022-04-01
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| Series: | Shiyan dongwu yu bijiao yixue |
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| Online Access: | https://www.slarc.org.cn/dwyx/CN/10.12300/j.issn.1674-5817.2021.082 |
| _version_ | 1797987030638002176 |
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| author | WU Chang LI Xiaojie WU Yinnan WANG Yao WANG Yuxin ZONG Weifeng MENG Changhong LU Yihong |
| author_facet | WU Chang LI Xiaojie WU Yinnan WANG Yao WANG Yuxin ZONG Weifeng MENG Changhong LU Yihong |
| author_sort | WU Chang |
| collection | DOAJ |
| description | ObjectiveTo evaluate the detection of the histamine substances in osteopeptide injections by the isolated guinea pig ileum method, and to explore the feasibility of replacing cat depressor substances method.MethodsAccording to the 2020 edition of Chinese Pharmacopoeia, the histamine substances in 7 batches of osteopeptide injections were detected from 5 enterprises by isolated guinea pig ileum method. The results were compared with the test method of cat depressor substances.ResultsBy the isolated guinea pig ileum method, the histamine substances in 3 batches of preparations were negative, which was consistent with the results of the test method of cat depressor substances. The results of the other 4 batches of preparations were suspicious; while the examination results of depressor substances in cat were 3 batches suspicious and 1 batch positive. The overall consistency rate of the two methods was 85.7% (6/7).ConclusionThe results of isolated guinea pig ileum method is almost the same as the test of cat depressor substances, which can be used for the screening and study of histamines in osteopeptide injections. It is suggested to add the inspection items of the test of depressor substances or histamines in the standard of osteopeptide injections. |
| first_indexed | 2024-04-11T07:42:25Z |
| format | Article |
| id | doaj.art-ffbd343476694cd8b48871e8b92f9576 |
| institution | Directory Open Access Journal |
| issn | 1674-5817 |
| language | zho |
| last_indexed | 2024-04-11T07:42:25Z |
| publishDate | 2022-04-01 |
| publisher | Editorial Office of Laboratory Animal and Comparative Medicine |
| record_format | Article |
| series | Shiyan dongwu yu bijiao yixue |
| spelling | doaj.art-ffbd343476694cd8b48871e8b92f95762022-12-22T04:36:30ZzhoEditorial Office of Laboratory Animal and Comparative MedicineShiyan dongwu yu bijiao yixue1674-58172022-04-0142215916510.12300/j.issn.1674-5817.2021.0821674-5817（2022）02-0159-07Application of Isolated Guinea Pig Ileum Method for Detection of Histamine Substances in Osteopeptide InjectionsWU Chang0LI Xiaojie1WU Yinnan2WANG Yao3WANG Yuxin4ZONG Weifeng5MENG Changhong6LU Yihong7Department of Pharmaceutical Analysis, Xuzhou Medical University, Xuzhou 221004, ChinaJiangsu Institute for Food and Drug Control, Nanjing 210019, ChinaDepartment of Pharmaceutical Analysis, Xuzhou Medical University, Xuzhou 221004, ChinaJiangsu Institute for Food and Drug Control, Nanjing 210019, ChinaJiangsu Institute for Food and Drug Control, Nanjing 210019, ChinaJiangsu Institute for Food and Drug Control, Nanjing 210019, ChinaJiangsu Institute for Food and Drug Control, Nanjing 210019, ChinaDepartment of Pharmaceutical Analysis, Xuzhou Medical University, Xuzhou 221004, ChinaObjectiveTo evaluate the detection of the histamine substances in osteopeptide injections by the isolated guinea pig ileum method, and to explore the feasibility of replacing cat depressor substances method.MethodsAccording to the 2020 edition of Chinese Pharmacopoeia, the histamine substances in 7 batches of osteopeptide injections were detected from 5 enterprises by isolated guinea pig ileum method. The results were compared with the test method of cat depressor substances.ResultsBy the isolated guinea pig ileum method, the histamine substances in 3 batches of preparations were negative, which was consistent with the results of the test method of cat depressor substances. The results of the other 4 batches of preparations were suspicious; while the examination results of depressor substances in cat were 3 batches suspicious and 1 batch positive. The overall consistency rate of the two methods was 85.7% (6/7).ConclusionThe results of isolated guinea pig ileum method is almost the same as the test of cat depressor substances, which can be used for the screening and study of histamines in osteopeptide injections. It is suggested to add the inspection items of the test of depressor substances or histamines in the standard of osteopeptide injections.https://www.slarc.org.cn/dwyx/CN/10.12300/j.issn.1674-5817.2021.082isolated guinea pig ileum methodosteopeptide injectionshistamine substances testtest of cat depressor substances |
| spellingShingle | WU Chang LI Xiaojie WU Yinnan WANG Yao WANG Yuxin ZONG Weifeng MENG Changhong LU Yihong Application of Isolated Guinea Pig Ileum Method for Detection of Histamine Substances in Osteopeptide Injections Shiyan dongwu yu bijiao yixue isolated guinea pig ileum method osteopeptide injections histamine substances test test of cat depressor substances |
| title | Application of Isolated Guinea Pig Ileum Method for Detection of Histamine Substances in Osteopeptide Injections |
| title_full | Application of Isolated Guinea Pig Ileum Method for Detection of Histamine Substances in Osteopeptide Injections |
| title_fullStr | Application of Isolated Guinea Pig Ileum Method for Detection of Histamine Substances in Osteopeptide Injections |
| title_full_unstemmed | Application of Isolated Guinea Pig Ileum Method for Detection of Histamine Substances in Osteopeptide Injections |
| title_short | Application of Isolated Guinea Pig Ileum Method for Detection of Histamine Substances in Osteopeptide Injections |
| title_sort | application of isolated guinea pig ileum method for detection of histamine substances in osteopeptide injections |
| topic | isolated guinea pig ileum method osteopeptide injections histamine substances test test of cat depressor substances |
| url | https://www.slarc.org.cn/dwyx/CN/10.12300/j.issn.1674-5817.2021.082 |
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