Determination of the Phenolic Profile, and Evaluation of Biological Activities of Hydroethanolic Extract from Aerial Parts of <i>Origanum compactum</i> from Morocco
<i>Origanum compactum</i> belonging to the family Lamiaceae is widely used in food and pharmaceutical fields due to its biologically active substances. We aimed to investigate the total phenol and flavonoid contents and the phenolic composition, and to evaluate the antioxidant and antiba...
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| Format: | Article |
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MDPI AG
2022-08-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/27/16/5189 |
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| author | Mounia Chroho Aziz Bouymajane Mustapha Aazza Yassine Oulad El Majdoub Francesco Cacciola Luigi Mondello Touriya Zair Latifa Bouissane |
| author_facet | Mounia Chroho Aziz Bouymajane Mustapha Aazza Yassine Oulad El Majdoub Francesco Cacciola Luigi Mondello Touriya Zair Latifa Bouissane |
| author_sort | Mounia Chroho |
| collection | DOAJ |
| description | <i>Origanum compactum</i> belonging to the family Lamiaceae is widely used in food and pharmaceutical fields due to its biologically active substances. We aimed to investigate the total phenol and flavonoid contents and the phenolic composition, and to evaluate the antioxidant and antibacterial properties of hydroethanolic extract from of <i>Origanum compactum</i>. Total phenol and flavonoid contents were evaluated using gallic acid and quercetin as standards, respectively, and the phenolic profile was characterized using high-performance liquid chromatography coupled to a photodiode array and electrospray ionization mass spectrometry (HPLC-PDA-ESI/MS). The antioxidant activity was determined by two methods: ferric reducing power (FRAP) assay and the phosphomolybdate method. The antibacterial effect was evaluated against four bacteria (<i>Escherichia coli</i>, <i>Salmonella typhimurium</i>, <i>Staphylococcus aureus</i> and <i>Listeria monocytogenes</i>) using the broth microdilution method. The findings show that the total phenolic and flavonoid contents were 107.789 ± 5.39 mg GAE/g dm and 14,977 ± 0.79 mg QE/g dm, respectively. A total of sixteen phenolic compounds belonging to phenolic acids and flavonoids were detected. Furthermore, the extract showed strong antioxidant activity, and displayed a bacteriostatic effect against <i>Escherichia coli</i> and <i>Salmonella typhimuriumn</i>, and a bactericidal effect against <i>Staphylococcus aureus</i> and <i>Listeria monocytogenes</i>. Therefore, this study reveals that <i>Origanum compactum</i> extracts display potential as antibacterial and natural antioxidant agents for fighting against pathogenic bacteria and preventing oxidative stress. |
| first_indexed | 2024-03-09T04:03:07Z |
| format | Article |
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| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-09T04:03:07Z |
| publishDate | 2022-08-01 |
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| record_format | Article |
| series | Molecules |
| spelling | doaj.art-ffc14cf512ee43448883b32db9fd7afc2023-12-03T14:11:26ZengMDPI AGMolecules1420-30492022-08-012716518910.3390/molecules27165189Determination of the Phenolic Profile, and Evaluation of Biological Activities of Hydroethanolic Extract from Aerial Parts of <i>Origanum compactum</i> from MoroccoMounia Chroho0Aziz Bouymajane1Mustapha Aazza2Yassine Oulad El Majdoub3Francesco Cacciola4Luigi Mondello5Touriya Zair6Latifa Bouissane7Molecular Chemistry, Materials and Catalysis Laboratory, Faculty of Sciences and Technologies, Sultan Moulay Slimane University, Beni-Mellal 23000, MoroccoTeam of Microbiology and Health, Laboratory of Chemistry-Biology Applied to the Environment, Faculty of Sciences, Moulay Ismail University, Zitoune, Meknes 11201, MoroccoLaboratory of Chemistry-Biology Applied to the Environment, Faculty of Sciences, Moulay Ismail University, Zitoune, Meknes 11201, MoroccoDepartment of Chemical, Biological, Pharmaceutical and Environmental Sciences, University of Messina, 98168 Messina, ItalyDepartment of Biomedical, Dental, Morphological and Functional Imaging Sciences, University of Messina, 98125 Messina, ItalyDepartment of Chemical, Biological, Pharmaceutical and Environmental Sciences, University of Messina, 98168 Messina, ItalyResearch Team Chemistry of Bioactive Molecules and Environment, Laboratory of Innovative Materials and Biotechnologies of Natural Resources, Faculty of Sciences, Moulay Ismail University, Zitoune, Meknes 11201, MoroccoMolecular Chemistry, Materials and Catalysis Laboratory, Faculty of Sciences and Technologies, Sultan Moulay Slimane University, Beni-Mellal 23000, Morocco<i>Origanum compactum</i> belonging to the family Lamiaceae is widely used in food and pharmaceutical fields due to its biologically active substances. We aimed to investigate the total phenol and flavonoid contents and the phenolic composition, and to evaluate the antioxidant and antibacterial properties of hydroethanolic extract from of <i>Origanum compactum</i>. Total phenol and flavonoid contents were evaluated using gallic acid and quercetin as standards, respectively, and the phenolic profile was characterized using high-performance liquid chromatography coupled to a photodiode array and electrospray ionization mass spectrometry (HPLC-PDA-ESI/MS). The antioxidant activity was determined by two methods: ferric reducing power (FRAP) assay and the phosphomolybdate method. The antibacterial effect was evaluated against four bacteria (<i>Escherichia coli</i>, <i>Salmonella typhimurium</i>, <i>Staphylococcus aureus</i> and <i>Listeria monocytogenes</i>) using the broth microdilution method. The findings show that the total phenolic and flavonoid contents were 107.789 ± 5.39 mg GAE/g dm and 14,977 ± 0.79 mg QE/g dm, respectively. A total of sixteen phenolic compounds belonging to phenolic acids and flavonoids were detected. Furthermore, the extract showed strong antioxidant activity, and displayed a bacteriostatic effect against <i>Escherichia coli</i> and <i>Salmonella typhimuriumn</i>, and a bactericidal effect against <i>Staphylococcus aureus</i> and <i>Listeria monocytogenes</i>. Therefore, this study reveals that <i>Origanum compactum</i> extracts display potential as antibacterial and natural antioxidant agents for fighting against pathogenic bacteria and preventing oxidative stress.https://www.mdpi.com/1420-3049/27/16/5189<i>Origanum compactum</i>phenolic compoundsHPLC-PDA-ESI/MSantioxidant activityantibacterial activity |
| spellingShingle | Mounia Chroho Aziz Bouymajane Mustapha Aazza Yassine Oulad El Majdoub Francesco Cacciola Luigi Mondello Touriya Zair Latifa Bouissane Determination of the Phenolic Profile, and Evaluation of Biological Activities of Hydroethanolic Extract from Aerial Parts of <i>Origanum compactum</i> from Morocco Molecules <i>Origanum compactum</i> phenolic compounds HPLC-PDA-ESI/MS antioxidant activity antibacterial activity |
| title | Determination of the Phenolic Profile, and Evaluation of Biological Activities of Hydroethanolic Extract from Aerial Parts of <i>Origanum compactum</i> from Morocco |
| title_full | Determination of the Phenolic Profile, and Evaluation of Biological Activities of Hydroethanolic Extract from Aerial Parts of <i>Origanum compactum</i> from Morocco |
| title_fullStr | Determination of the Phenolic Profile, and Evaluation of Biological Activities of Hydroethanolic Extract from Aerial Parts of <i>Origanum compactum</i> from Morocco |
| title_full_unstemmed | Determination of the Phenolic Profile, and Evaluation of Biological Activities of Hydroethanolic Extract from Aerial Parts of <i>Origanum compactum</i> from Morocco |
| title_short | Determination of the Phenolic Profile, and Evaluation of Biological Activities of Hydroethanolic Extract from Aerial Parts of <i>Origanum compactum</i> from Morocco |
| title_sort | determination of the phenolic profile and evaluation of biological activities of hydroethanolic extract from aerial parts of i origanum compactum i from morocco |
| topic | <i>Origanum compactum</i> phenolic compounds HPLC-PDA-ESI/MS antioxidant activity antibacterial activity |
| url | https://www.mdpi.com/1420-3049/27/16/5189 |
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