Development and Validation of RP-HPLC Method for the Determination of Adefovir Dipivoxil in Bulk and in Pharmaceutical Formulation
A rapid and sensitive RP-HPLC method with UV detection (262 nm) for routine analysis of adefovir dipivoxil in bulk and in pharmaceutical formulation was developed. Chromatography was performed with mobile phase containing a mixture of acetonitrile and phosphate buffer (50:50, v/v) with flow rate 1.0...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Hindawi Limited
2009-01-01
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| Series: | E-Journal of Chemistry |
| Online Access: | http://dx.doi.org/10.1155/2009/157868 |
| _version_ | 1798043102630379520 |
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| author | Zaheer Ahmed B. Gopinath A. Sathish Kumar Shetty B. K. Sridhar |
| author_facet | Zaheer Ahmed B. Gopinath A. Sathish Kumar Shetty B. K. Sridhar |
| author_sort | Zaheer Ahmed |
| collection | DOAJ |
| description | A rapid and sensitive RP-HPLC method with UV detection (262 nm) for routine analysis of adefovir dipivoxil in bulk and in pharmaceutical formulation was developed. Chromatography was performed with mobile phase containing a mixture of acetonitrile and phosphate buffer (50:50, v/v) with flow rate 1.0 mL min-l. In the range of 5.0-100 µg/mL, the linearity of adefovir dipivoxil shows a correlation co-efficient of 0.9999. The proposed method was validated by determining sensitivity accuracy, precision, robustness stability, specificity, selectivity and system suitability parameters. |
| first_indexed | 2024-04-11T22:44:55Z |
| format | Article |
| id | doaj.art-ffc2633646d5433ea35f026506fd1650 |
| institution | Directory Open Access Journal |
| issn | 0973-4945 2090-9810 |
| language | English |
| last_indexed | 2024-04-11T22:44:55Z |
| publishDate | 2009-01-01 |
| publisher | Hindawi Limited |
| record_format | Article |
| series | E-Journal of Chemistry |
| spelling | doaj.art-ffc2633646d5433ea35f026506fd16502022-12-22T03:58:48ZengHindawi LimitedE-Journal of Chemistry0973-49452090-98102009-01-016246947410.1155/2009/157868Development and Validation of RP-HPLC Method for the Determination of Adefovir Dipivoxil in Bulk and in Pharmaceutical FormulationZaheer Ahmed0B. Gopinath1A. Sathish Kumar Shetty2B. K. Sridhar3Department of Pharmaceutical Analysis, National College of Pharmacy, Shimoga-577201, (K.S.), IndiaDepartment of Pharmaceutical Analysis, National College of Pharmacy, Shimoga-577201, (K.S.), IndiaDepartment of Pharmaceutical Analysis, National College of Pharmacy, Shimoga-577201, (K.S.), IndiaDepartment of Pharmaceutical Analysis, National College of Pharmacy, Shimoga-577201, (K.S.), IndiaA rapid and sensitive RP-HPLC method with UV detection (262 nm) for routine analysis of adefovir dipivoxil in bulk and in pharmaceutical formulation was developed. Chromatography was performed with mobile phase containing a mixture of acetonitrile and phosphate buffer (50:50, v/v) with flow rate 1.0 mL min-l. In the range of 5.0-100 µg/mL, the linearity of adefovir dipivoxil shows a correlation co-efficient of 0.9999. The proposed method was validated by determining sensitivity accuracy, precision, robustness stability, specificity, selectivity and system suitability parameters.http://dx.doi.org/10.1155/2009/157868 |
| spellingShingle | Zaheer Ahmed B. Gopinath A. Sathish Kumar Shetty B. K. Sridhar Development and Validation of RP-HPLC Method for the Determination of Adefovir Dipivoxil in Bulk and in Pharmaceutical Formulation E-Journal of Chemistry |
| title | Development and Validation of RP-HPLC Method for the Determination of Adefovir Dipivoxil in Bulk and in Pharmaceutical Formulation |
| title_full | Development and Validation of RP-HPLC Method for the Determination of Adefovir Dipivoxil in Bulk and in Pharmaceutical Formulation |
| title_fullStr | Development and Validation of RP-HPLC Method for the Determination of Adefovir Dipivoxil in Bulk and in Pharmaceutical Formulation |
| title_full_unstemmed | Development and Validation of RP-HPLC Method for the Determination of Adefovir Dipivoxil in Bulk and in Pharmaceutical Formulation |
| title_short | Development and Validation of RP-HPLC Method for the Determination of Adefovir Dipivoxil in Bulk and in Pharmaceutical Formulation |
| title_sort | development and validation of rp hplc method for the determination of adefovir dipivoxil in bulk and in pharmaceutical formulation |
| url | http://dx.doi.org/10.1155/2009/157868 |
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