Protective effects of thiamine on Wickerhamomyces anomalus against ethanol stress
Wickerhamomyces anomalus (W. anomalus) is widely reported in the brewing industry and has positive effects on the aromatic profiles of wines because of its unique physiological characteristics and metabolic features. However, the accumulation of ethanol during fermentation inhibits the growth of W....
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2022-12-01
|
| Series: | Frontiers in Microbiology |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2022.1057284/full |
| _version_ | 1811178035601211392 |
|---|---|
| author | Yinfeng Li Hua Long Guilan Jiang Zhihai Yu Mingzheng Huang Shiping Zou Tianbing Guan Yan Zhao Xiaozhu Liu Xiaozhu Liu |
| author_facet | Yinfeng Li Hua Long Guilan Jiang Zhihai Yu Mingzheng Huang Shiping Zou Tianbing Guan Yan Zhao Xiaozhu Liu Xiaozhu Liu |
| author_sort | Yinfeng Li |
| collection | DOAJ |
| description | Wickerhamomyces anomalus (W. anomalus) is widely reported in the brewing industry and has positive effects on the aromatic profiles of wines because of its unique physiological characteristics and metabolic features. However, the accumulation of ethanol during fermentation inhibits the growth of W. anomalus. Thiamine is involved in the response against various abiotic stresses in microorganisms. Therefore, we used transcriptomic and metabolomic analyses to study the effect of thiamine on ethanol-stressed W. anomalus. The results indicate that thiamine could alleviate the inhibitory effect of ethanol stress on the survival of W. anomalus. Differentially expressed genes (DEGs) and differentially expressed metabolites (DEMs) caused by the thiamine intervention were identified as oxidative phosphorylation through integrated transcriptomic and metabolomic analyses. In addition, ethanol treatment decreased the content of intracellular adenosine triphosphate (ATP), while thiamine partially alleviated this phenomenon. The present comprehensive transcriptional overview and metabolomic analysis provide insights about the mechanisms of thiamine protection on W. anomalus under ethanol stress and promote the potential applications of W. anomalus in the fermentation industry. |
| first_indexed | 2024-04-11T06:11:30Z |
| format | Article |
| id | doaj.art-ffc2c06f32c94d6f913d6934834a6618 |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-04-11T06:11:30Z |
| publishDate | 2022-12-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-ffc2c06f32c94d6f913d6934834a66182022-12-22T04:41:14ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2022-12-011310.3389/fmicb.2022.10572841057284Protective effects of thiamine on Wickerhamomyces anomalus against ethanol stressYinfeng Li0Hua Long1Guilan Jiang2Zhihai Yu3Mingzheng Huang4Shiping Zou5Tianbing Guan6Yan Zhao7Xiaozhu Liu8Xiaozhu Liu9Guizhou Institute of Technology, Guiyang, ChinaGuizhou Institute of Technology, Guiyang, ChinaGuizhou Institute of Technology, Guiyang, ChinaGuizhou Institute of Technology, Guiyang, ChinaGuizhou Institute of Technology, Guiyang, ChinaGuizhou Institute of Technology, Guiyang, ChinaChongqing Key Laboratory of Industrial Fermentation Microorganism, Chongqing University of Science and Technology, Chongqing, ChinaCollege of Bioscience and Biotechnology, Hunan Agricultural University, Changsha, ChinaGuizhou Institute of Technology, Guiyang, ChinaChongqing Key Laboratory of Industrial Fermentation Microorganism, Chongqing University of Science and Technology, Chongqing, ChinaWickerhamomyces anomalus (W. anomalus) is widely reported in the brewing industry and has positive effects on the aromatic profiles of wines because of its unique physiological characteristics and metabolic features. However, the accumulation of ethanol during fermentation inhibits the growth of W. anomalus. Thiamine is involved in the response against various abiotic stresses in microorganisms. Therefore, we used transcriptomic and metabolomic analyses to study the effect of thiamine on ethanol-stressed W. anomalus. The results indicate that thiamine could alleviate the inhibitory effect of ethanol stress on the survival of W. anomalus. Differentially expressed genes (DEGs) and differentially expressed metabolites (DEMs) caused by the thiamine intervention were identified as oxidative phosphorylation through integrated transcriptomic and metabolomic analyses. In addition, ethanol treatment decreased the content of intracellular adenosine triphosphate (ATP), while thiamine partially alleviated this phenomenon. The present comprehensive transcriptional overview and metabolomic analysis provide insights about the mechanisms of thiamine protection on W. anomalus under ethanol stress and promote the potential applications of W. anomalus in the fermentation industry.https://www.frontiersin.org/articles/10.3389/fmicb.2022.1057284/fullethanol stressthiamineWickerhamomyces anomalustranscriptomicsmetabolomics |
| spellingShingle | Yinfeng Li Hua Long Guilan Jiang Zhihai Yu Mingzheng Huang Shiping Zou Tianbing Guan Yan Zhao Xiaozhu Liu Xiaozhu Liu Protective effects of thiamine on Wickerhamomyces anomalus against ethanol stress Frontiers in Microbiology ethanol stress thiamine Wickerhamomyces anomalus transcriptomics metabolomics |
| title | Protective effects of thiamine on Wickerhamomyces anomalus against ethanol stress |
| title_full | Protective effects of thiamine on Wickerhamomyces anomalus against ethanol stress |
| title_fullStr | Protective effects of thiamine on Wickerhamomyces anomalus against ethanol stress |
| title_full_unstemmed | Protective effects of thiamine on Wickerhamomyces anomalus against ethanol stress |
| title_short | Protective effects of thiamine on Wickerhamomyces anomalus against ethanol stress |
| title_sort | protective effects of thiamine on wickerhamomyces anomalus against ethanol stress |
| topic | ethanol stress thiamine Wickerhamomyces anomalus transcriptomics metabolomics |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2022.1057284/full |
| work_keys_str_mv | AT yinfengli protectiveeffectsofthiamineonwickerhamomycesanomalusagainstethanolstress AT hualong protectiveeffectsofthiamineonwickerhamomycesanomalusagainstethanolstress AT guilanjiang protectiveeffectsofthiamineonwickerhamomycesanomalusagainstethanolstress AT zhihaiyu protectiveeffectsofthiamineonwickerhamomycesanomalusagainstethanolstress AT mingzhenghuang protectiveeffectsofthiamineonwickerhamomycesanomalusagainstethanolstress AT shipingzou protectiveeffectsofthiamineonwickerhamomycesanomalusagainstethanolstress AT tianbingguan protectiveeffectsofthiamineonwickerhamomycesanomalusagainstethanolstress AT yanzhao protectiveeffectsofthiamineonwickerhamomycesanomalusagainstethanolstress AT xiaozhuliu protectiveeffectsofthiamineonwickerhamomycesanomalusagainstethanolstress AT xiaozhuliu protectiveeffectsofthiamineonwickerhamomycesanomalusagainstethanolstress |