In Vitro Anti-Inflammatory Study of Limonoids Isolated from <i>Chisocheton</i> Plants
<i>Chisocheton</i> plants from the family Meliaceae have traditionally been used to treat several diseases; however, scientific evidence is limited. The most abundant chemical constituents of this plant are the limonoids, which are known for their various biological activities, including...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2024-01-01
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| Series: | Current Issues in Molecular Biology |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1467-3045/46/1/58 |
| Summary: | <i>Chisocheton</i> plants from the family Meliaceae have traditionally been used to treat several diseases; however, scientific evidence is limited. The most abundant chemical constituents of this plant are the limonoids, which are known for their various biological activities, including anti-inflammatory effects. However, the anti-inflammatory effects and underlying mechanisms of action of the constituents of <i>Chisocheton</i> plants have not been fully explored. In this report, we evaluated the anti-inflammatory activity of 17 limonoid compounds from <i>Chisocheton</i> plant primarily by measuring their inhibitory effects on the production of pro-inflammatory cytokines, including TNF-α, IL-6, IL-1β, and MCP-1, in LPS-stimulated THP-1 cells using an ELISA assay. Compounds <b>3</b>, <b>5</b>, <b>9</b>, and <b>14</b>–<b>17</b> exhibited significant activity in inhibiting the evaluated pro-inflammatory markers, with IC<sub>50</sub> values less than 20 µM and a high selectivity index (SI) range. Compounds <b>3</b>, <b>5</b>, <b>9</b>, and <b>15</b> significantly suppressed the expression of phosphorylated p38 MAPK in THP-1 cells stimulated with LPS. These findings support the use of limonoids from <i>Chisocheton</i> plants as promising candidates for anti-inflammatory therapy. |
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| ISSN: | 1467-3037 1467-3045 |