Pretreatment with H<sub>2</sub>O<sub>2</sub> Alleviates the Negative Impacts of NaCl Stress on Seed Germination of Tartary Buckwheat (<i>Fagopyrum tataricum</i>)
Soil salinization is one of the main abiotic stress factors impacting the growth of crops and the agricultural industry today. Thus, we aimed to investigate the effects of H<sub>2</sub>O<sub>2</sub> pretreatment on seed germination in Tartary buckwheat (<i>Fagopyrum tat...
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MDPI AG
2021-08-01
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| Series: | Plants |
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| Online Access: | https://www.mdpi.com/2223-7747/10/9/1784 |
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| author | Xin Yao Meiliang Zhou Jingjun Ruan Yan Peng Hao Yang Yong Tang Anjing Gao Jianping Cheng |
| author_facet | Xin Yao Meiliang Zhou Jingjun Ruan Yan Peng Hao Yang Yong Tang Anjing Gao Jianping Cheng |
| author_sort | Xin Yao |
| collection | DOAJ |
| description | Soil salinization is one of the main abiotic stress factors impacting the growth of crops and the agricultural industry today. Thus, we aimed to investigate the effects of H<sub>2</sub>O<sub>2</sub> pretreatment on seed germination in Tartary buckwheat (<i>Fagopyrum tataricum</i>) seeds under salt stress and to evaluate this species’ salt tolerance. Through the preliminary experiment, this study used 50 mmol L<sup>−1</sup> NaCl solution to induce seed stress. After soaking for 12 h in different H<sub>2</sub>O<sub>2</sub> concentrations, seeds were laid in Petri dishes with 50 mmol L<sup>−1</sup> NaCl for seven days and the germination parameters and physiological indicators were measured to screen the optimal H<sub>2</sub>O<sub>2</sub> pretreatment concentration and the salt tolerance index. Our results indicated that pretreatment with 5–10 mmol L<sup>−1</sup> H<sub>2</sub>O<sub>2</sub> was most effective in alleviating NaCl’s impacts on the seeds’ germination parameters. Furthermore, the growth and material accumulation of seedlings was promoted; catalase, superoxide dismutase activity, and proline content were enhanced; and malondialdehyde content was reduced. Principal component analysis and stepwise regression revealed six key indicators that had a significant impact on the salt tolerance characteristics of <i>F. tataricum</i>, namely, germination potential, shoot fresh weight, root surface area, root average diameter, catalase activity, and superoxide dismutase activity. |
| first_indexed | 2024-03-10T07:17:56Z |
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| language | English |
| last_indexed | 2024-03-10T07:17:56Z |
| publishDate | 2021-08-01 |
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| series | Plants |
| spelling | doaj.art-ffc48be7eaec46108c5f8062035f6d262023-11-22T14:51:40ZengMDPI AGPlants2223-77472021-08-01109178410.3390/plants10091784Pretreatment with H<sub>2</sub>O<sub>2</sub> Alleviates the Negative Impacts of NaCl Stress on Seed Germination of Tartary Buckwheat (<i>Fagopyrum tataricum</i>)Xin Yao0Meiliang Zhou1Jingjun Ruan2Yan Peng3Hao Yang4Yong Tang5Anjing Gao6Jianping Cheng7College of Agronomy, Guizhou University, Guiyang 550000, ChinaInstitute of Crop Science, Chinese Academy of Agriculture Science, Beijing 100000, ChinaCollege of Agronomy, Guizhou University, Guiyang 550000, ChinaCollege of Agronomy, Guizhou University, Guiyang 550000, ChinaCollege of Agronomy, Guizhou University, Guiyang 550000, ChinaCollege of Agronomy, Guizhou University, Guiyang 550000, ChinaCollege of Agronomy, Guizhou University, Guiyang 550000, ChinaCollege of Agronomy, Guizhou University, Guiyang 550000, ChinaSoil salinization is one of the main abiotic stress factors impacting the growth of crops and the agricultural industry today. Thus, we aimed to investigate the effects of H<sub>2</sub>O<sub>2</sub> pretreatment on seed germination in Tartary buckwheat (<i>Fagopyrum tataricum</i>) seeds under salt stress and to evaluate this species’ salt tolerance. Through the preliminary experiment, this study used 50 mmol L<sup>−1</sup> NaCl solution to induce seed stress. After soaking for 12 h in different H<sub>2</sub>O<sub>2</sub> concentrations, seeds were laid in Petri dishes with 50 mmol L<sup>−1</sup> NaCl for seven days and the germination parameters and physiological indicators were measured to screen the optimal H<sub>2</sub>O<sub>2</sub> pretreatment concentration and the salt tolerance index. Our results indicated that pretreatment with 5–10 mmol L<sup>−1</sup> H<sub>2</sub>O<sub>2</sub> was most effective in alleviating NaCl’s impacts on the seeds’ germination parameters. Furthermore, the growth and material accumulation of seedlings was promoted; catalase, superoxide dismutase activity, and proline content were enhanced; and malondialdehyde content was reduced. Principal component analysis and stepwise regression revealed six key indicators that had a significant impact on the salt tolerance characteristics of <i>F. tataricum</i>, namely, germination potential, shoot fresh weight, root surface area, root average diameter, catalase activity, and superoxide dismutase activity.https://www.mdpi.com/2223-7747/10/9/1784<i>F. tataricum</i>germinationhydrogen peroxideNaClseed soaking |
| spellingShingle | Xin Yao Meiliang Zhou Jingjun Ruan Yan Peng Hao Yang Yong Tang Anjing Gao Jianping Cheng Pretreatment with H<sub>2</sub>O<sub>2</sub> Alleviates the Negative Impacts of NaCl Stress on Seed Germination of Tartary Buckwheat (<i>Fagopyrum tataricum</i>) Plants <i>F. tataricum</i> germination hydrogen peroxide NaCl seed soaking |
| title | Pretreatment with H<sub>2</sub>O<sub>2</sub> Alleviates the Negative Impacts of NaCl Stress on Seed Germination of Tartary Buckwheat (<i>Fagopyrum tataricum</i>) |
| title_full | Pretreatment with H<sub>2</sub>O<sub>2</sub> Alleviates the Negative Impacts of NaCl Stress on Seed Germination of Tartary Buckwheat (<i>Fagopyrum tataricum</i>) |
| title_fullStr | Pretreatment with H<sub>2</sub>O<sub>2</sub> Alleviates the Negative Impacts of NaCl Stress on Seed Germination of Tartary Buckwheat (<i>Fagopyrum tataricum</i>) |
| title_full_unstemmed | Pretreatment with H<sub>2</sub>O<sub>2</sub> Alleviates the Negative Impacts of NaCl Stress on Seed Germination of Tartary Buckwheat (<i>Fagopyrum tataricum</i>) |
| title_short | Pretreatment with H<sub>2</sub>O<sub>2</sub> Alleviates the Negative Impacts of NaCl Stress on Seed Germination of Tartary Buckwheat (<i>Fagopyrum tataricum</i>) |
| title_sort | pretreatment with h sub 2 sub o sub 2 sub alleviates the negative impacts of nacl stress on seed germination of tartary buckwheat i fagopyrum tataricum i |
| topic | <i>F. tataricum</i> germination hydrogen peroxide NaCl seed soaking |
| url | https://www.mdpi.com/2223-7747/10/9/1784 |
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