Potential serodiagnostic markers for Q fever identified in <b><it>Coxiella burnetii </it></b>by immunoproteomic and protein microarray approaches
<p>Abstract</p> <p>Background</p> <p><it>Coxiella burnetii </it>is the etiological agent of Q fever. The clinical diagnosis of Q fever is mainly based on several serological tests. These tests all need Coxiella organisms which are difficult and hazardous to...
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| Format: | Article |
| Language: | English |
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BMC
2012-03-01
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| Series: | BMC Microbiology |
| Online Access: | http://www.biomedcentral.com/1471-2180/12/35 |
| _version_ | 1818789728517881856 |
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| author | Xiong Xiaolu Wang Xile Wen Bohai Graves Stephen Stenos John |
| author_facet | Xiong Xiaolu Wang Xile Wen Bohai Graves Stephen Stenos John |
| author_sort | Xiong Xiaolu |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p><it>Coxiella burnetii </it>is the etiological agent of Q fever. The clinical diagnosis of Q fever is mainly based on several serological tests. These tests all need Coxiella organisms which are difficult and hazardous to culture and purify.</p> <p>Results</p> <p>An immunoproteomic study of <it>C. burnetii </it>Xinqiao strain isolated in China was conducted with the sera from experimentally infected BALB/c mice and Q fever patients. Twenty of whole proteins of Xinqiao recognized by the infection sera were identified by mass spectrometry. Nineteen of the 20 proteins were successfully expressed in <it>Escherichia coli </it>and used to fabricate a microarray which was probed with Q fever patient sera. As a result, GroEL, YbgF, RplL, Mip, OmpH, Com1, and Dnak were recognized as major seroreactive antigens. The major seroreactive proteins were fabricated in a small microarray and further analyzed with the sera of patients with rickettsial spotted fever, Legionella pneumonia or streptococcal pneumonia. In this analysis, these proteins showed fewer cross-reactions with the tested sera.</p> <p>Conclusions</p> <p>Our results demonstrate that these 7 Coxiella proteins gave a modest sensitivity and specificity for recognizing of Q fever patient sera, suggesting that they are potential serodiagnostic markers for Q fever.</p> |
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| format | Article |
| id | doaj.art-ffc4d0d1bd794a5193707288646d432d |
| institution | Directory Open Access Journal |
| issn | 1471-2180 |
| language | English |
| last_indexed | 2024-12-18T14:44:11Z |
| publishDate | 2012-03-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Microbiology |
| spelling | doaj.art-ffc4d0d1bd794a5193707288646d432d2022-12-21T21:04:19ZengBMCBMC Microbiology1471-21802012-03-011213510.1186/1471-2180-12-35Potential serodiagnostic markers for Q fever identified in <b><it>Coxiella burnetii </it></b>by immunoproteomic and protein microarray approachesXiong XiaoluWang XileWen BohaiGraves StephenStenos John<p>Abstract</p> <p>Background</p> <p><it>Coxiella burnetii </it>is the etiological agent of Q fever. The clinical diagnosis of Q fever is mainly based on several serological tests. These tests all need Coxiella organisms which are difficult and hazardous to culture and purify.</p> <p>Results</p> <p>An immunoproteomic study of <it>C. burnetii </it>Xinqiao strain isolated in China was conducted with the sera from experimentally infected BALB/c mice and Q fever patients. Twenty of whole proteins of Xinqiao recognized by the infection sera were identified by mass spectrometry. Nineteen of the 20 proteins were successfully expressed in <it>Escherichia coli </it>and used to fabricate a microarray which was probed with Q fever patient sera. As a result, GroEL, YbgF, RplL, Mip, OmpH, Com1, and Dnak were recognized as major seroreactive antigens. The major seroreactive proteins were fabricated in a small microarray and further analyzed with the sera of patients with rickettsial spotted fever, Legionella pneumonia or streptococcal pneumonia. In this analysis, these proteins showed fewer cross-reactions with the tested sera.</p> <p>Conclusions</p> <p>Our results demonstrate that these 7 Coxiella proteins gave a modest sensitivity and specificity for recognizing of Q fever patient sera, suggesting that they are potential serodiagnostic markers for Q fever.</p>http://www.biomedcentral.com/1471-2180/12/35 |
| spellingShingle | Xiong Xiaolu Wang Xile Wen Bohai Graves Stephen Stenos John Potential serodiagnostic markers for Q fever identified in <b><it>Coxiella burnetii </it></b>by immunoproteomic and protein microarray approaches BMC Microbiology |
| title | Potential serodiagnostic markers for Q fever identified in <b><it>Coxiella burnetii </it></b>by immunoproteomic and protein microarray approaches |
| title_full | Potential serodiagnostic markers for Q fever identified in <b><it>Coxiella burnetii </it></b>by immunoproteomic and protein microarray approaches |
| title_fullStr | Potential serodiagnostic markers for Q fever identified in <b><it>Coxiella burnetii </it></b>by immunoproteomic and protein microarray approaches |
| title_full_unstemmed | Potential serodiagnostic markers for Q fever identified in <b><it>Coxiella burnetii </it></b>by immunoproteomic and protein microarray approaches |
| title_short | Potential serodiagnostic markers for Q fever identified in <b><it>Coxiella burnetii </it></b>by immunoproteomic and protein microarray approaches |
| title_sort | potential serodiagnostic markers for q fever identified in b it coxiella burnetii it b by immunoproteomic and protein microarray approaches |
| url | http://www.biomedcentral.com/1471-2180/12/35 |
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