Evidence for infection but not transmission of Zika virus by Aedes albopictus (Diptera: Culicidae) from Spain
Abstract Background A number of mosquito-borne viruses such as dengue virus (DENV), Usutu virus (USUV), West Nile virus (WNV) are autochthonously transmitted in Europe and six invasive mosquito species have been detected in this temperate region. This has increased the risk for the emergence of furt...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2019-05-01
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| Series: | Parasites & Vectors |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s13071-019-3467-y |
| _version_ | 1818502886433226752 |
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| author | Luis M. Hernández-Triana Elsa Barrero Sarah Delacour-Estrella Ignacio Ruiz-Arrondo Javier Lucientes Maria del Mar Fernández de Marco Leigh Thorne Sarah Lumley Nicholas Johnson Karen L. Mansfield Anthony R. Fooks |
| author_facet | Luis M. Hernández-Triana Elsa Barrero Sarah Delacour-Estrella Ignacio Ruiz-Arrondo Javier Lucientes Maria del Mar Fernández de Marco Leigh Thorne Sarah Lumley Nicholas Johnson Karen L. Mansfield Anthony R. Fooks |
| author_sort | Luis M. Hernández-Triana |
| collection | DOAJ |
| description | Abstract Background A number of mosquito-borne viruses such as dengue virus (DENV), Usutu virus (USUV), West Nile virus (WNV) are autochthonously transmitted in Europe and six invasive mosquito species have been detected in this temperate region. This has increased the risk for the emergence of further mosquito-borne diseases. However, there is a paucity of information on whether European populations of invasive mosquito species are competent to transmit arboviruses. In this study, the susceptibility of Aedes albopictus originating from Spain and a laboratory-adapted colony of Aedes aegypti, was assessed for infection with, and transmission of Zika virus (ZIKV). Vertical transmission in both species was also assessed. Methods Aedes albopictus colonised from eggs collected in Spain and an existing colony of Ae. aegypti were fed infectious blood meals containing ZIKV (Polynesian strain) at 1.6 × 107 PFU/ml. Blood-fed mosquitoes were separated and maintained at 20 °C or 25 °C. Legs, saliva and bodies were sampled from specimens at 7, 14 and 21 days post-infection (dpi) in order to determine infection, dissemination and transmission rates. All samples were analysed by real-time RT-PCR using primers targeting the ZIKV NS1 gene. Results At 14 dpi and 21 dpi, ZIKV RNA was detected in the bodies of both species at both temperatures. However, live virus only was detected in the saliva of Ae. aegypti at 25 °C with a transmission rate of 44%. No evidence for virus expectoration was obtained for Ae. albopictus under any condition. Notably, ZIKV RNA was not detectable in the saliva of Ae. aegypti at 20 °C after 21 days. No vertical transmission of ZIKV was detected in this study. Conclusions Experimental infection of Ae. albopictus colonized from Spain with ZIKV did not result in expectoration of virus in saliva in contrast to results for Ae. aegypti. No evidence of vertical transmission of virus was observed in this study. This suggests that this strain of Ae. albopictus is not competent for ZIKV transmission under the conditions tested. |
| first_indexed | 2024-12-10T21:16:24Z |
| format | Article |
| id | doaj.art-ffc88f7530e2484286709e8467e63a63 |
| institution | Directory Open Access Journal |
| issn | 1756-3305 |
| language | English |
| last_indexed | 2024-12-10T21:16:24Z |
| publishDate | 2019-05-01 |
| publisher | BMC |
| record_format | Article |
| series | Parasites & Vectors |
| spelling | doaj.art-ffc88f7530e2484286709e8467e63a632022-12-22T01:33:17ZengBMCParasites & Vectors1756-33052019-05-011211610.1186/s13071-019-3467-yEvidence for infection but not transmission of Zika virus by Aedes albopictus (Diptera: Culicidae) from SpainLuis M. Hernández-Triana0Elsa Barrero1Sarah Delacour-Estrella2Ignacio Ruiz-Arrondo3Javier Lucientes4Maria del Mar Fernández de Marco5Leigh Thorne6Sarah Lumley7Nicholas Johnson8Karen L. Mansfield9Anthony R. Fooks10Wildlife Zoonoses and Vector-borne Diseases Research Group, Animal and Plant Health AgencyWildlife Zoonoses and Vector-borne Diseases Research Group, Animal and Plant Health AgencyDepartment of Animal Pathology, Faculty of Veterinary Medicine, University of ZaragozaCenter for Rickettsiosis and Vector-Borne Diseases Group, Hospital Universitario San Pedro-CIBIRDepartment of Animal Pathology, Faculty of Veterinary Medicine, University of ZaragozaWildlife Zoonoses and Vector-borne Diseases Research Group, Animal and Plant Health AgencyWildlife Zoonoses and Vector-borne Diseases Research Group, Animal and Plant Health AgencyPublic Health EnglandWildlife Zoonoses and Vector-borne Diseases Research Group, Animal and Plant Health AgencyWildlife Zoonoses and Vector-borne Diseases Research Group, Animal and Plant Health AgencyWildlife Zoonoses and Vector-borne Diseases Research Group, Animal and Plant Health AgencyAbstract Background A number of mosquito-borne viruses such as dengue virus (DENV), Usutu virus (USUV), West Nile virus (WNV) are autochthonously transmitted in Europe and six invasive mosquito species have been detected in this temperate region. This has increased the risk for the emergence of further mosquito-borne diseases. However, there is a paucity of information on whether European populations of invasive mosquito species are competent to transmit arboviruses. In this study, the susceptibility of Aedes albopictus originating from Spain and a laboratory-adapted colony of Aedes aegypti, was assessed for infection with, and transmission of Zika virus (ZIKV). Vertical transmission in both species was also assessed. Methods Aedes albopictus colonised from eggs collected in Spain and an existing colony of Ae. aegypti were fed infectious blood meals containing ZIKV (Polynesian strain) at 1.6 × 107 PFU/ml. Blood-fed mosquitoes were separated and maintained at 20 °C or 25 °C. Legs, saliva and bodies were sampled from specimens at 7, 14 and 21 days post-infection (dpi) in order to determine infection, dissemination and transmission rates. All samples were analysed by real-time RT-PCR using primers targeting the ZIKV NS1 gene. Results At 14 dpi and 21 dpi, ZIKV RNA was detected in the bodies of both species at both temperatures. However, live virus only was detected in the saliva of Ae. aegypti at 25 °C with a transmission rate of 44%. No evidence for virus expectoration was obtained for Ae. albopictus under any condition. Notably, ZIKV RNA was not detectable in the saliva of Ae. aegypti at 20 °C after 21 days. No vertical transmission of ZIKV was detected in this study. Conclusions Experimental infection of Ae. albopictus colonized from Spain with ZIKV did not result in expectoration of virus in saliva in contrast to results for Ae. aegypti. No evidence of vertical transmission of virus was observed in this study. This suggests that this strain of Ae. albopictus is not competent for ZIKV transmission under the conditions tested.http://link.springer.com/article/10.1186/s13071-019-3467-yAedes aegyptiAedes albopictusZika virusVector competenceSpain |
| spellingShingle | Luis M. Hernández-Triana Elsa Barrero Sarah Delacour-Estrella Ignacio Ruiz-Arrondo Javier Lucientes Maria del Mar Fernández de Marco Leigh Thorne Sarah Lumley Nicholas Johnson Karen L. Mansfield Anthony R. Fooks Evidence for infection but not transmission of Zika virus by Aedes albopictus (Diptera: Culicidae) from Spain Parasites & Vectors Aedes aegypti Aedes albopictus Zika virus Vector competence Spain |
| title | Evidence for infection but not transmission of Zika virus by Aedes albopictus (Diptera: Culicidae) from Spain |
| title_full | Evidence for infection but not transmission of Zika virus by Aedes albopictus (Diptera: Culicidae) from Spain |
| title_fullStr | Evidence for infection but not transmission of Zika virus by Aedes albopictus (Diptera: Culicidae) from Spain |
| title_full_unstemmed | Evidence for infection but not transmission of Zika virus by Aedes albopictus (Diptera: Culicidae) from Spain |
| title_short | Evidence for infection but not transmission of Zika virus by Aedes albopictus (Diptera: Culicidae) from Spain |
| title_sort | evidence for infection but not transmission of zika virus by aedes albopictus diptera culicidae from spain |
| topic | Aedes aegypti Aedes albopictus Zika virus Vector competence Spain |
| url | http://link.springer.com/article/10.1186/s13071-019-3467-y |
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