<i>Bta-miR-484</i> Targets <i>SFRP1</i> and Affects Preadipocytes Proliferation, Differentiation, and Apoptosis
MicroRNAs (miRNAs) are essential regulators of numerous biological processes in animals, including adipogenesis. Despite the abundance of miRNAs associated with adipogenesis, their exact mechanisms of action remain largely unknown. Our study highlights the role of <i>bta-miR-484</i> as a...
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MDPI AG
2023-08-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/24/16/12710 |
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| author | Mengli Yang Xiaoqian Gao Chunli Hu Shuzhe Wang Hui Sheng Yun Ma |
| author_facet | Mengli Yang Xiaoqian Gao Chunli Hu Shuzhe Wang Hui Sheng Yun Ma |
| author_sort | Mengli Yang |
| collection | DOAJ |
| description | MicroRNAs (miRNAs) are essential regulators of numerous biological processes in animals, including adipogenesis. Despite the abundance of miRNAs associated with adipogenesis, their exact mechanisms of action remain largely unknown. Our study highlights the role of <i>bta-miR-484</i> as a major regulator of adipocyte proliferation, apoptosis, and differentiation. Here, we demonstrated that the expression of <i>bta-miR-484</i> initially increased during adipogenesis before decreasing. Overexpression of <i>bta-miR-484</i> in adipocytes ultimately inhibited cell proliferation and differentiation, reduced the number of EdU fluorescence-stained cells, increased the number of G1 phase cells, reduced the number of G2 and S phase cells, and downregulated the expression of proliferation markers (<i>CDK2</i> and <i>PCNA</i>) and differentiation markers (<i>CEBPA</i>, <i>FABP4</i>, and <i>LPL</i>). Additionally, overexpression of <i>bta-miR-484</i> promoted the expression of apoptosis-related genes (<i>Caspase 3</i>, <i>Caspase 9</i>, and <i>BAX</i>), and increased the number of apoptotic cells observed via flow cytometry. In contrast, <i>bta-miR-484</i> inhibition in adipocytes yielded opposite effects to those observed during <i>bta-miR-484</i> overexpression. Moreover, luciferase reporter assays confirmed <i>SFRP1</i> as a target gene of <i>bta-miR-484</i>, and revealed that <i>bta-miR-484</i> downregulates <i>SFRP1</i> mRNA expression. These findings offer compelling evidence that <i>bta-miR-484</i> targets <i>SFRP1</i>, inhibits proliferation and differentiation, and promotes apoptosis. Therefore, these results offer novel insights into the <i>bta-miR-484</i> regulation of adipocyte growth and development. |
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| language | English |
| last_indexed | 2024-03-10T23:52:58Z |
| publishDate | 2023-08-01 |
| publisher | MDPI AG |
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| series | International Journal of Molecular Sciences |
| spelling | doaj.art-ffc89268df4c4511b9bba44b98c402492023-11-19T01:27:54ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672023-08-0124161271010.3390/ijms241612710<i>Bta-miR-484</i> Targets <i>SFRP1</i> and Affects Preadipocytes Proliferation, Differentiation, and ApoptosisMengli Yang0Xiaoqian Gao1Chunli Hu2Shuzhe Wang3Hui Sheng4Yun Ma5Key Laboratory of Ruminant Molecular and Cellular Breeding of Ningxia Hui Autonomous Region, College of Animal Science and Technology, Ningxia University, Yinchuan 750021, ChinaKey Laboratory of Ruminant Molecular and Cellular Breeding of Ningxia Hui Autonomous Region, College of Animal Science and Technology, Ningxia University, Yinchuan 750021, ChinaKey Laboratory of Ruminant Molecular and Cellular Breeding of Ningxia Hui Autonomous Region, College of Animal Science and Technology, Ningxia University, Yinchuan 750021, ChinaKey Laboratory of Ruminant Molecular and Cellular Breeding of Ningxia Hui Autonomous Region, College of Animal Science and Technology, Ningxia University, Yinchuan 750021, ChinaKey Laboratory of Ruminant Molecular and Cellular Breeding of Ningxia Hui Autonomous Region, College of Animal Science and Technology, Ningxia University, Yinchuan 750021, ChinaKey Laboratory of Ruminant Molecular and Cellular Breeding of Ningxia Hui Autonomous Region, College of Animal Science and Technology, Ningxia University, Yinchuan 750021, ChinaMicroRNAs (miRNAs) are essential regulators of numerous biological processes in animals, including adipogenesis. Despite the abundance of miRNAs associated with adipogenesis, their exact mechanisms of action remain largely unknown. Our study highlights the role of <i>bta-miR-484</i> as a major regulator of adipocyte proliferation, apoptosis, and differentiation. Here, we demonstrated that the expression of <i>bta-miR-484</i> initially increased during adipogenesis before decreasing. Overexpression of <i>bta-miR-484</i> in adipocytes ultimately inhibited cell proliferation and differentiation, reduced the number of EdU fluorescence-stained cells, increased the number of G1 phase cells, reduced the number of G2 and S phase cells, and downregulated the expression of proliferation markers (<i>CDK2</i> and <i>PCNA</i>) and differentiation markers (<i>CEBPA</i>, <i>FABP4</i>, and <i>LPL</i>). Additionally, overexpression of <i>bta-miR-484</i> promoted the expression of apoptosis-related genes (<i>Caspase 3</i>, <i>Caspase 9</i>, and <i>BAX</i>), and increased the number of apoptotic cells observed via flow cytometry. In contrast, <i>bta-miR-484</i> inhibition in adipocytes yielded opposite effects to those observed during <i>bta-miR-484</i> overexpression. Moreover, luciferase reporter assays confirmed <i>SFRP1</i> as a target gene of <i>bta-miR-484</i>, and revealed that <i>bta-miR-484</i> downregulates <i>SFRP1</i> mRNA expression. These findings offer compelling evidence that <i>bta-miR-484</i> targets <i>SFRP1</i>, inhibits proliferation and differentiation, and promotes apoptosis. Therefore, these results offer novel insights into the <i>bta-miR-484</i> regulation of adipocyte growth and development.https://www.mdpi.com/1422-0067/24/16/12710<i>bta-miR-484</i><i>SFRP1</i>bovine preadipocytesdifferentiationproliferation |
| spellingShingle | Mengli Yang Xiaoqian Gao Chunli Hu Shuzhe Wang Hui Sheng Yun Ma <i>Bta-miR-484</i> Targets <i>SFRP1</i> and Affects Preadipocytes Proliferation, Differentiation, and Apoptosis International Journal of Molecular Sciences <i>bta-miR-484</i> <i>SFRP1</i> bovine preadipocytes differentiation proliferation |
| title | <i>Bta-miR-484</i> Targets <i>SFRP1</i> and Affects Preadipocytes Proliferation, Differentiation, and Apoptosis |
| title_full | <i>Bta-miR-484</i> Targets <i>SFRP1</i> and Affects Preadipocytes Proliferation, Differentiation, and Apoptosis |
| title_fullStr | <i>Bta-miR-484</i> Targets <i>SFRP1</i> and Affects Preadipocytes Proliferation, Differentiation, and Apoptosis |
| title_full_unstemmed | <i>Bta-miR-484</i> Targets <i>SFRP1</i> and Affects Preadipocytes Proliferation, Differentiation, and Apoptosis |
| title_short | <i>Bta-miR-484</i> Targets <i>SFRP1</i> and Affects Preadipocytes Proliferation, Differentiation, and Apoptosis |
| title_sort | i bta mir 484 i targets i sfrp1 i and affects preadipocytes proliferation differentiation and apoptosis |
| topic | <i>bta-miR-484</i> <i>SFRP1</i> bovine preadipocytes differentiation proliferation |
| url | https://www.mdpi.com/1422-0067/24/16/12710 |
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