A Phylogenomic Analysis of the Floral Transcriptomes of Sexually Deceptive and Rewarding European Orchids, Ophrys and Gymnadenia
The orchids (Orchidaceae) constitute one of the largest and most diverse families of flowering plants. They have evolved a great variety of adaptations to achieve pollination by a diverse group of pollinators. Many orchids reward their pollinators, typically with nectar, but the family is also well-...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2019-11-01
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| Series: | Frontiers in Plant Science |
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| Online Access: | https://www.frontiersin.org/article/10.3389/fpls.2019.01553/full |
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| author | Laura Piñeiro Fernández Laura Piñeiro Fernández Kelsey J. R .P. Byers Kelsey J. R .P. Byers Jing Cai Jing Cai Khalid E. M. Sedeek Khalid E. M. Sedeek Khalid E. M. Sedeek Roman T. Kellenberger Roman T. Kellenberger Alessia Russo Alessia Russo Alessia Russo Weihong Qi Catharine Aquino Fournier Philipp M. Schlüter |
| author_facet | Laura Piñeiro Fernández Laura Piñeiro Fernández Kelsey J. R .P. Byers Kelsey J. R .P. Byers Jing Cai Jing Cai Khalid E. M. Sedeek Khalid E. M. Sedeek Khalid E. M. Sedeek Roman T. Kellenberger Roman T. Kellenberger Alessia Russo Alessia Russo Alessia Russo Weihong Qi Catharine Aquino Fournier Philipp M. Schlüter |
| author_sort | Laura Piñeiro Fernández |
| collection | DOAJ |
| description | The orchids (Orchidaceae) constitute one of the largest and most diverse families of flowering plants. They have evolved a great variety of adaptations to achieve pollination by a diverse group of pollinators. Many orchids reward their pollinators, typically with nectar, but the family is also well-known for employing deceptive pollination strategies in which there is no reward for the pollinator, in the most extreme case by mimicking sexual signals of pollinators. In the European flora, two examples of these different pollination strategies are the sexually deceptive genus Ophrys and the rewarding genus Gymnadenia, which differ in their level of pollinator specialization; Ophrys is typically pollinated by pseudo-copulation of males of a single insect species, whilst Gymnadenia attracts a broad range of floral visitors. Here, we present and describe the annotated floral transcriptome of Ophrys iricolor, an Andrena-pollinated representative of the genus Ophrys that is widespread throughout the Aegean. Furthermore, we present additional floral transcriptomes of both sexually deceptive and rewarding orchids, specifically the deceptive Ophrys insectifera, Ophrys aymoninii, and an updated floral transcriptome of Ophrys sphegodes, as well as the floral transcriptomes of the rewarding orchids Gymnadenia conopsea, Gymnadenia densiflora, Gymnadenia odoratissima, and Gymnadenia rhellicani (syn. Nigritella rhellicani). Comparisons of these novel floral transcriptomes reveal few annotation differences between deceptive and rewarding orchids. Since together, these transcriptomes provide a representative sample of the genus-wide taxonomic diversity within Ophrys and Gymnadenia (Orchidoideae: Orchidinae), we employ a phylogenomic approach to address open questions of phylogenetic relationships within the genera. Specifically, this includes the controversial placement of O. insectifera within the Ophrys phylogeny and the placement of “Nigritella”-type morphologies within the phylogeny of Gymnadenia. Whereas in Gymnadenia, several conflicting topologies are supported by a similar number of gene trees, a majority of Ophrys gene topologies clearly supports a placement of O. insectifera as sister to a clade containing O. sphegodes. |
| first_indexed | 2024-12-21T23:05:32Z |
| format | Article |
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| institution | Directory Open Access Journal |
| issn | 1664-462X |
| language | English |
| last_indexed | 2024-12-21T23:05:32Z |
| publishDate | 2019-11-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Plant Science |
| spelling | doaj.art-ffccbbfd9443471eb203e535006104de2022-12-21T18:47:11ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2019-11-011010.3389/fpls.2019.01553486233A Phylogenomic Analysis of the Floral Transcriptomes of Sexually Deceptive and Rewarding European Orchids, Ophrys and GymnadeniaLaura Piñeiro Fernández0Laura Piñeiro Fernández1Kelsey J. R .P. Byers2Kelsey J. R .P. Byers3Jing Cai4Jing Cai5Khalid E. M. Sedeek6Khalid E. M. Sedeek7Khalid E. M. Sedeek8Roman T. Kellenberger9Roman T. Kellenberger10Alessia Russo11Alessia Russo12Alessia Russo13Weihong Qi14Catharine Aquino Fournier15Philipp M. Schlüter16Institute of Botany, University of Hohenheim, Stuttgart, GermanyDepartment of Systematic and Evolutionary Botany, University of Zurich, Zurich, SwitzerlandDepartment of Systematic and Evolutionary Botany, University of Zurich, Zurich, SwitzerlandDepartment of Zoology, University of Cambridge, Cambridge, United KingdomDepartment of Systematic and Evolutionary Botany, University of Zurich, Zurich, SwitzerlandCenter for Ecological and Environmental Sciences, Northwestern Polytechnical University, Xi’an, ChinaDepartment of Systematic and Evolutionary Botany, University of Zurich, Zurich, SwitzerlandLaboratory for Genome Engineering and Synthetic Biology, Division of Biological Sciences, King Abdullah University of Science and Technology, Thuwal, Saudi ArabiaAgricultural Genetic Engineering Research Institute (AGERI), Agriculture Research Centre, Giza, EgyptDepartment of Systematic and Evolutionary Botany, University of Zurich, Zurich, SwitzerlandDepartment of Plant Sciences, University of Cambridge, Cambridge, United KingdomInstitute of Botany, University of Hohenheim, Stuttgart, GermanyDepartment of Systematic and Evolutionary Botany, University of Zurich, Zurich, SwitzerlandDepartment of Plant and Microbial Biology, University of Zurich, Zurich, SwitzerlandFunctional Genomics Centre Zurich, Zurich, SwitzerlandFunctional Genomics Centre Zurich, Zurich, SwitzerlandInstitute of Botany, University of Hohenheim, Stuttgart, GermanyThe orchids (Orchidaceae) constitute one of the largest and most diverse families of flowering plants. They have evolved a great variety of adaptations to achieve pollination by a diverse group of pollinators. Many orchids reward their pollinators, typically with nectar, but the family is also well-known for employing deceptive pollination strategies in which there is no reward for the pollinator, in the most extreme case by mimicking sexual signals of pollinators. In the European flora, two examples of these different pollination strategies are the sexually deceptive genus Ophrys and the rewarding genus Gymnadenia, which differ in their level of pollinator specialization; Ophrys is typically pollinated by pseudo-copulation of males of a single insect species, whilst Gymnadenia attracts a broad range of floral visitors. Here, we present and describe the annotated floral transcriptome of Ophrys iricolor, an Andrena-pollinated representative of the genus Ophrys that is widespread throughout the Aegean. Furthermore, we present additional floral transcriptomes of both sexually deceptive and rewarding orchids, specifically the deceptive Ophrys insectifera, Ophrys aymoninii, and an updated floral transcriptome of Ophrys sphegodes, as well as the floral transcriptomes of the rewarding orchids Gymnadenia conopsea, Gymnadenia densiflora, Gymnadenia odoratissima, and Gymnadenia rhellicani (syn. Nigritella rhellicani). Comparisons of these novel floral transcriptomes reveal few annotation differences between deceptive and rewarding orchids. Since together, these transcriptomes provide a representative sample of the genus-wide taxonomic diversity within Ophrys and Gymnadenia (Orchidoideae: Orchidinae), we employ a phylogenomic approach to address open questions of phylogenetic relationships within the genera. Specifically, this includes the controversial placement of O. insectifera within the Ophrys phylogeny and the placement of “Nigritella”-type morphologies within the phylogeny of Gymnadenia. Whereas in Gymnadenia, several conflicting topologies are supported by a similar number of gene trees, a majority of Ophrys gene topologies clearly supports a placement of O. insectifera as sister to a clade containing O. sphegodes.https://www.frontiersin.org/article/10.3389/fpls.2019.01553/fullphylogenomicsorchidsOphrysGymnadeniatranscriptomepollination strategy |
| spellingShingle | Laura Piñeiro Fernández Laura Piñeiro Fernández Kelsey J. R .P. Byers Kelsey J. R .P. Byers Jing Cai Jing Cai Khalid E. M. Sedeek Khalid E. M. Sedeek Khalid E. M. Sedeek Roman T. Kellenberger Roman T. Kellenberger Alessia Russo Alessia Russo Alessia Russo Weihong Qi Catharine Aquino Fournier Philipp M. Schlüter A Phylogenomic Analysis of the Floral Transcriptomes of Sexually Deceptive and Rewarding European Orchids, Ophrys and Gymnadenia Frontiers in Plant Science phylogenomics orchids Ophrys Gymnadenia transcriptome pollination strategy |
| title | A Phylogenomic Analysis of the Floral Transcriptomes of Sexually Deceptive and Rewarding European Orchids, Ophrys and Gymnadenia |
| title_full | A Phylogenomic Analysis of the Floral Transcriptomes of Sexually Deceptive and Rewarding European Orchids, Ophrys and Gymnadenia |
| title_fullStr | A Phylogenomic Analysis of the Floral Transcriptomes of Sexually Deceptive and Rewarding European Orchids, Ophrys and Gymnadenia |
| title_full_unstemmed | A Phylogenomic Analysis of the Floral Transcriptomes of Sexually Deceptive and Rewarding European Orchids, Ophrys and Gymnadenia |
| title_short | A Phylogenomic Analysis of the Floral Transcriptomes of Sexually Deceptive and Rewarding European Orchids, Ophrys and Gymnadenia |
| title_sort | phylogenomic analysis of the floral transcriptomes of sexually deceptive and rewarding european orchids ophrys and gymnadenia |
| topic | phylogenomics orchids Ophrys Gymnadenia transcriptome pollination strategy |
| url | https://www.frontiersin.org/article/10.3389/fpls.2019.01553/full |
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