Measurement of yeast concentration in rectangular and cylindrical cuvette
Knowledge of yeast concentration is necessary for the pitching rate estimation. Centrifugation and turbidimetric method present rapid and easy methods for the determination of yeast concentration. There are many variants of such techniques to monitor laboratory fermentations even in test tube sizes....
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| Format: | Article |
| Language: | ces |
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Research Institute of Brewing and Malting, Plc.
2018-10-01
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| Series: | Kvasný průmysl |
| Subjects: | |
| Online Access: | http://www.kvasnyprumysl.eu/index.php/kp/article/view/27 |
| _version_ | 1811327774596530176 |
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| author | Jan Šavel |
| author_facet | Jan Šavel |
| author_sort | Jan Šavel |
| collection | DOAJ |
| description | Knowledge of yeast concentration is necessary for the pitching rate estimation. Centrifugation and turbidimetric method present rapid and easy methods for the determination of yeast concentration. There are many variants of such techniques to monitor laboratory fermentations even in test tube sizes. Procedures with and without sodium hydroxide solution were compared and evaluated. A simple calculation procedure is based on two parameters, which have to be determined for the local condition of each brewery. They cover yeast dry matter content of yeast sample and the yeast count related to 1 g completely dry yeast. It enables to calculate the probable cell count in pitched substrate, which can be used for the photometric calibration curve construction. Linear and logarithmic relation was used to express relation between yeast concentration and absorbance at 800 nm. The method based on yeast deflocculation enabled to estimate starting yeast activity. |
| first_indexed | 2024-04-13T15:14:01Z |
| format | Article |
| id | doaj.art-ffd5485542bf4b57a596a6dd102cac6f |
| institution | Directory Open Access Journal |
| issn | 2570-8619 |
| language | ces |
| last_indexed | 2024-04-13T15:14:01Z |
| publishDate | 2018-10-01 |
| publisher | Research Institute of Brewing and Malting, Plc. |
| record_format | Article |
| series | Kvasný průmysl |
| spelling | doaj.art-ffd5485542bf4b57a596a6dd102cac6f2022-12-22T02:41:56ZcesResearch Institute of Brewing and Malting, Plc.Kvasný průmysl2570-86192018-10-0164521722310.18832/kp20182527Measurement of yeast concentration in rectangular and cylindrical cuvetteJan Šavel0Budweiser Budvar, N.C., Karolíny Světlé 4, 370 21 České BudějoviceKnowledge of yeast concentration is necessary for the pitching rate estimation. Centrifugation and turbidimetric method present rapid and easy methods for the determination of yeast concentration. There are many variants of such techniques to monitor laboratory fermentations even in test tube sizes. Procedures with and without sodium hydroxide solution were compared and evaluated. A simple calculation procedure is based on two parameters, which have to be determined for the local condition of each brewery. They cover yeast dry matter content of yeast sample and the yeast count related to 1 g completely dry yeast. It enables to calculate the probable cell count in pitched substrate, which can be used for the photometric calibration curve construction. Linear and logarithmic relation was used to express relation between yeast concentration and absorbance at 800 nm. The method based on yeast deflocculation enabled to estimate starting yeast activity.http://www.kvasnyprumysl.eu/index.php/kp/article/view/27centrifugationdeflocullation testphotometrypressed yeastyeast activityyeast concentrationyeast pitchingyeast trub |
| spellingShingle | Jan Šavel Measurement of yeast concentration in rectangular and cylindrical cuvette Kvasný průmysl centrifugation deflocullation test photometry pressed yeast yeast activity yeast concentration yeast pitching yeast trub |
| title | Measurement of yeast concentration in rectangular and cylindrical cuvette |
| title_full | Measurement of yeast concentration in rectangular and cylindrical cuvette |
| title_fullStr | Measurement of yeast concentration in rectangular and cylindrical cuvette |
| title_full_unstemmed | Measurement of yeast concentration in rectangular and cylindrical cuvette |
| title_short | Measurement of yeast concentration in rectangular and cylindrical cuvette |
| title_sort | measurement of yeast concentration in rectangular and cylindrical cuvette |
| topic | centrifugation deflocullation test photometry pressed yeast yeast activity yeast concentration yeast pitching yeast trub |
| url | http://www.kvasnyprumysl.eu/index.php/kp/article/view/27 |
| work_keys_str_mv | AT jansavel measurementofyeastconcentrationinrectangularandcylindricalcuvette |