Callus induction and regeneration in high-altitude Himalayan rice genotype SR4 via seed explant
SR4 genotype of rice is high altitude Himalayan rice prone to various abiotic stresses such as cold stress and therefore gives a poor yield. An efficient protocol for callusing and regeneration via direct and indirect means was established using mature seeds as an explant which can be utilized for m...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2022-12-01
|
| Series: | Biotechnology Reports |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2215017X22000601 |
| _version_ | 1811274432050626560 |
|---|---|
| author | Wasifa Noor Rafiq Lone Azra N. Kamili Amjad M. Husaini |
| author_facet | Wasifa Noor Rafiq Lone Azra N. Kamili Amjad M. Husaini |
| author_sort | Wasifa Noor |
| collection | DOAJ |
| description | SR4 genotype of rice is high altitude Himalayan rice prone to various abiotic stresses such as cold stress and therefore gives a poor yield. An efficient protocol for callusing and regeneration via direct and indirect means was established using mature seeds as an explant which can be utilized for molecular studies for genetic advancement of Himalayan rice genotype SR4 through transformation. Highest frequency (96.6%) of callus induction was obtained on MS media 3.0 mg/L 2, 4-D. While maximum regeneration frequency (100%), number of shoots with maximum length 9.14 ± 0.204 (cm) from callus was recovered from MS media amended with 5.0 mg/L BAP in combination with 0.5 mg/L NAA with highest number of shoots having an average shoot length 9.14 ± 0.204 (cm) after four weeks of culture. Direct multiple shoot regeneration from seed explants was obtained using various concentrations of TDZ and BAP with highest regeneration frequency was observed on MS media fortified with 6 mg/L of TDZ with maximum number of shoots. The shoots developed roots on MS media supplemented with 0.6 mg/L IBA. |
| first_indexed | 2024-04-12T23:18:55Z |
| format | Article |
| id | doaj.art-ffdafa74f705433ba301e89b21d9d833 |
| institution | Directory Open Access Journal |
| issn | 2215-017X |
| language | English |
| last_indexed | 2024-04-12T23:18:55Z |
| publishDate | 2022-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Biotechnology Reports |
| spelling | doaj.art-ffdafa74f705433ba301e89b21d9d8332022-12-22T03:12:35ZengElsevierBiotechnology Reports2215-017X2022-12-0136e00762Callus induction and regeneration in high-altitude Himalayan rice genotype SR4 via seed explantWasifa Noor0Rafiq Lone1Azra N. Kamili2Amjad M. Husaini3Centre of Research for Development, University of Kashmir, Srinagar-190006, IndiaDepartment of Botany, Central University of Kashmir, Ganderbal, Jammu and Kashmir, India; Corresponding author.Department of Botany, Central University of Kashmir, Ganderbal, Jammu and Kashmir, IndiaDivision of Plant Biotechnology, SKUAST-K Shalimar, Srinagar-190025, IndiaSR4 genotype of rice is high altitude Himalayan rice prone to various abiotic stresses such as cold stress and therefore gives a poor yield. An efficient protocol for callusing and regeneration via direct and indirect means was established using mature seeds as an explant which can be utilized for molecular studies for genetic advancement of Himalayan rice genotype SR4 through transformation. Highest frequency (96.6%) of callus induction was obtained on MS media 3.0 mg/L 2, 4-D. While maximum regeneration frequency (100%), number of shoots with maximum length 9.14 ± 0.204 (cm) from callus was recovered from MS media amended with 5.0 mg/L BAP in combination with 0.5 mg/L NAA with highest number of shoots having an average shoot length 9.14 ± 0.204 (cm) after four weeks of culture. Direct multiple shoot regeneration from seed explants was obtained using various concentrations of TDZ and BAP with highest regeneration frequency was observed on MS media fortified with 6 mg/L of TDZ with maximum number of shoots. The shoots developed roots on MS media supplemented with 0.6 mg/L IBA.http://www.sciencedirect.com/science/article/pii/S2215017X22000601Callus inductionHimalayanRegeneration frequencySR4 |
| spellingShingle | Wasifa Noor Rafiq Lone Azra N. Kamili Amjad M. Husaini Callus induction and regeneration in high-altitude Himalayan rice genotype SR4 via seed explant Biotechnology Reports Callus induction Himalayan Regeneration frequency SR4 |
| title | Callus induction and regeneration in high-altitude Himalayan rice genotype SR4 via seed explant |
| title_full | Callus induction and regeneration in high-altitude Himalayan rice genotype SR4 via seed explant |
| title_fullStr | Callus induction and regeneration in high-altitude Himalayan rice genotype SR4 via seed explant |
| title_full_unstemmed | Callus induction and regeneration in high-altitude Himalayan rice genotype SR4 via seed explant |
| title_short | Callus induction and regeneration in high-altitude Himalayan rice genotype SR4 via seed explant |
| title_sort | callus induction and regeneration in high altitude himalayan rice genotype sr4 via seed explant |
| topic | Callus induction Himalayan Regeneration frequency SR4 |
| url | http://www.sciencedirect.com/science/article/pii/S2215017X22000601 |
| work_keys_str_mv | AT wasifanoor callusinductionandregenerationinhighaltitudehimalayanricegenotypesr4viaseedexplant AT rafiqlone callusinductionandregenerationinhighaltitudehimalayanricegenotypesr4viaseedexplant AT azrankamili callusinductionandregenerationinhighaltitudehimalayanricegenotypesr4viaseedexplant AT amjadmhusaini callusinductionandregenerationinhighaltitudehimalayanricegenotypesr4viaseedexplant |