Development of Silk Fibroin-Based Non-Crosslinking Thermosensitive Bioinks for 3D Bioprinting
Three-dimensional (3D) bioprinting holds great promise for tissue engineering, allowing cells to thrive in a 3D environment. However, the applicability of natural polymers such as silk fibroin (SF) in 3D bioprinting faces hurdles due to limited mechanical strength and printability. SF, derived from...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-08-01
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| Series: | Polymers |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2073-4360/15/17/3567 |
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| author | Juo Lee Sangbae Park Sungmin Lee Hae Yong Kweon You-Young Jo Jungsil Kim Jong Hoon Chung Hoon Seonwoo |
| author_facet | Juo Lee Sangbae Park Sungmin Lee Hae Yong Kweon You-Young Jo Jungsil Kim Jong Hoon Chung Hoon Seonwoo |
| author_sort | Juo Lee |
| collection | DOAJ |
| description | Three-dimensional (3D) bioprinting holds great promise for tissue engineering, allowing cells to thrive in a 3D environment. However, the applicability of natural polymers such as silk fibroin (SF) in 3D bioprinting faces hurdles due to limited mechanical strength and printability. SF, derived from the silkworm Bombyx mori, is emerging as a potential bioink due to its inherent physical gelling properties. However, research on inducing thermosensitive behavior in SF-based bioinks and tailoring their mechanical properties to specific tissue requirements is notably lacking. This study addresses these gaps through the development of silk fibroin-based thermosensitive bioinks (SF-TPBs). Precise modulation of gelation time and mechanical robustness is achieved by manipulating glycerol content without recourse to cross-linkers. Chemical analysis confirms β-sheet conformation in SF-TPBs independent of glycerol concentration. Increased glycerol content improves gelation kinetics and results in rheological properties suitable for 3D printing. Overall, SF-TPBs offer promising prospects for realizing the potential of 3D bioprinting using natural polymers. |
| first_indexed | 2024-03-10T23:14:57Z |
| format | Article |
| id | doaj.art-ffdb0a3ad112458b818560aa12efb187 |
| institution | Directory Open Access Journal |
| issn | 2073-4360 |
| language | English |
| last_indexed | 2024-03-10T23:14:57Z |
| publishDate | 2023-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Polymers |
| spelling | doaj.art-ffdb0a3ad112458b818560aa12efb1872023-11-19T08:43:25ZengMDPI AGPolymers2073-43602023-08-011517356710.3390/polym15173567Development of Silk Fibroin-Based Non-Crosslinking Thermosensitive Bioinks for 3D BioprintingJuo Lee0Sangbae Park1Sungmin Lee2Hae Yong Kweon3You-Young Jo4Jungsil Kim5Jong Hoon Chung6Hoon Seonwoo7Department of Animal Science & Technology, Sunchon National University, Suncheon 57922, Republic of KoreaDepartment of Convergence Biosystems Engineering, Chonnam National University, Gwangju 61186, Republic of KoreaDepartment of Mechanical Engineering, Sunchon National University, Suncheon 57922, Republic of KoreaDivision of Industrial Insect and Sericulture, National Institute of Agricultural Sciences, Rural Development Administration, Wanju 55365, Republic of KoreaDepartment of Agricultural Biology, Apiculture Division, National Institute of Agricultural Sciences, Rural Development Administration, Wanju 55365, Republic of KoreaDepartment of Bio-Industrial Machinery Engineering, Kyungpook National University, 80 Daehakro, Bukgu, Daegu 41566, Republic of KoreaELBIO Inc., Seoul 08812, Republic of KoreaInterdisciplinary Program in IT-Bio Convergence System, Sunchon National University, Suncheon 57922, Republic of KoreaThree-dimensional (3D) bioprinting holds great promise for tissue engineering, allowing cells to thrive in a 3D environment. However, the applicability of natural polymers such as silk fibroin (SF) in 3D bioprinting faces hurdles due to limited mechanical strength and printability. SF, derived from the silkworm Bombyx mori, is emerging as a potential bioink due to its inherent physical gelling properties. However, research on inducing thermosensitive behavior in SF-based bioinks and tailoring their mechanical properties to specific tissue requirements is notably lacking. This study addresses these gaps through the development of silk fibroin-based thermosensitive bioinks (SF-TPBs). Precise modulation of gelation time and mechanical robustness is achieved by manipulating glycerol content without recourse to cross-linkers. Chemical analysis confirms β-sheet conformation in SF-TPBs independent of glycerol concentration. Increased glycerol content improves gelation kinetics and results in rheological properties suitable for 3D printing. Overall, SF-TPBs offer promising prospects for realizing the potential of 3D bioprinting using natural polymers.https://www.mdpi.com/2073-4360/15/17/3567silk fibroinbioprintingbioink |
| spellingShingle | Juo Lee Sangbae Park Sungmin Lee Hae Yong Kweon You-Young Jo Jungsil Kim Jong Hoon Chung Hoon Seonwoo Development of Silk Fibroin-Based Non-Crosslinking Thermosensitive Bioinks for 3D Bioprinting Polymers silk fibroin bioprinting bioink |
| title | Development of Silk Fibroin-Based Non-Crosslinking Thermosensitive Bioinks for 3D Bioprinting |
| title_full | Development of Silk Fibroin-Based Non-Crosslinking Thermosensitive Bioinks for 3D Bioprinting |
| title_fullStr | Development of Silk Fibroin-Based Non-Crosslinking Thermosensitive Bioinks for 3D Bioprinting |
| title_full_unstemmed | Development of Silk Fibroin-Based Non-Crosslinking Thermosensitive Bioinks for 3D Bioprinting |
| title_short | Development of Silk Fibroin-Based Non-Crosslinking Thermosensitive Bioinks for 3D Bioprinting |
| title_sort | development of silk fibroin based non crosslinking thermosensitive bioinks for 3d bioprinting |
| topic | silk fibroin bioprinting bioink |
| url | https://www.mdpi.com/2073-4360/15/17/3567 |
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