Cyclic thrombocytopenia associated with estradiol: a case report
ABSTRACTObjectives Cyclic thrombocytopenia (CTP) is a rare blood disorder characterized by periodic fluctuations in platelet counts. CTP usually appears in pre-menopausal women, and these fluctuations of platelets are in phase with the menstrual cycle. CTP is a heterogeneous disease, and the pathoge...
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2023-12-01
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| Series: | Hematology |
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| Online Access: | https://www.tandfonline.com/doi/10.1080/16078454.2023.2240140 |
| _version_ | 1797772010729766912 |
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| author | Ningbo Pang Yingwei Li Kangxi Zhou Chunliang Liu Rong Yan Chenglin Sun Weiling Xiao Changgeng Ruan Zhimin Zhai Kesheng Dai |
| author_facet | Ningbo Pang Yingwei Li Kangxi Zhou Chunliang Liu Rong Yan Chenglin Sun Weiling Xiao Changgeng Ruan Zhimin Zhai Kesheng Dai |
| author_sort | Ningbo Pang |
| collection | DOAJ |
| description | ABSTRACTObjectives Cyclic thrombocytopenia (CTP) is a rare blood disorder characterized by periodic fluctuations in platelet counts. CTP usually appears in pre-menopausal women, and these fluctuations of platelets are in phase with the menstrual cycle. CTP is a heterogeneous disease, and the pathogenic mechanism is still unclear. Therefore, it harbors great significance for exploring the association of fluctuations in platelet counts with hormonal-cycle.Materials Firstly, we washed human platelets from healthy volunteers following the Declaration of Helsinki. Flow cytometer was employed to measure the mitochondrial inner transmembrane potential (ΔΨm) depolarization, PS exposure, P-selectin expression, and GPIIb/IIIa activation in platelets. In addition, western blot detected the related protein expression. The corresponding assay kit measured the caspase-3 and PDE3A activity. Finally, flow cytometry determined mouse platelets labeled with calcein.Results We find a reverse relationship between the platelet count and serum estradiol (E2) level in a CTP patient. We demonstrated that E2 induces platelet apoptosis in vitro and platelet clearance in vivo. We further discovered that E2 activates phosphodiesterase 3A, which inhibits protein kinase A (PKA), leading to PKA-mediated platelet apoptosis. Activation of PKA protected platelets from E2-induced thrombocytopenia and elevated the number of mice circulatory platelets.Conclusions We find that E2 induces platelet apoptosis and clearance through PDE3A-mediated PKA inhibition. Activation of PKA rescues E2-induced thrombocytopenia in mice. Thus, our study reveals a pathogenesis of E2-related CTP and suggests promising therapeutic strategies for the disease. |
| first_indexed | 2024-03-12T21:44:53Z |
| format | Article |
| id | doaj.art-ffe06407db2c413294f4abf0fbf57fe2 |
| institution | Directory Open Access Journal |
| issn | 1607-8454 |
| language | English |
| last_indexed | 2024-03-12T21:44:53Z |
| publishDate | 2023-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Hematology |
| spelling | doaj.art-ffe06407db2c413294f4abf0fbf57fe22023-07-26T13:06:05ZengTaylor & Francis GroupHematology1607-84542023-12-0128110.1080/16078454.2023.2240140Cyclic thrombocytopenia associated with estradiol: a case reportNingbo Pang0Yingwei Li1Kangxi Zhou2Chunliang Liu3Rong Yan4Chenglin Sun5Weiling Xiao6Changgeng Ruan7Zhimin Zhai8Kesheng Dai9Jiangsu Institute of Hematology, Cyrus Tang Medical Institute, The First Affiliated Hospital and Collaborative Innovation Center of Hematology, State Key Laboratory of Radiation Medicine and Protection, Suzhou Medical College, Soochow University, Key Laboratory of Thrombosis and Hemostasis, Ministry of Health, National Clinical Research Center for Hematological Diseases, Suzhou, People’s Republic of ChinaHematology Department of the Second Hospital of Anhui Medical University, Hematologic Diseases Research Center of Anhui Medical University, Hefei, People’s Republic of ChinaJiangsu Institute of Hematology, Cyrus Tang Medical Institute, The First Affiliated Hospital and Collaborative Innovation Center of Hematology, State Key Laboratory of Radiation Medicine and Protection, Suzhou Medical College, Soochow University, Key Laboratory of Thrombosis and Hemostasis, Ministry of Health, National Clinical Research Center for Hematological Diseases, Suzhou, People’s Republic of ChinaJiangsu Institute of Hematology, Cyrus Tang Medical Institute, The First Affiliated Hospital and Collaborative Innovation Center of Hematology, State Key Laboratory of Radiation Medicine and Protection, Suzhou Medical College, Soochow University, Key Laboratory of Thrombosis and Hemostasis, Ministry of Health, National Clinical Research Center for Hematological Diseases, Suzhou, People’s Republic of ChinaJiangsu Institute of Hematology, Cyrus Tang Medical Institute, The First Affiliated Hospital and Collaborative Innovation Center of Hematology, State Key Laboratory of Radiation Medicine and Protection, Suzhou Medical College, Soochow University, Key Laboratory of Thrombosis and Hemostasis, Ministry of Health, National Clinical Research Center for Hematological Diseases, Suzhou, People’s Republic of ChinaJiangsu Institute of Hematology, Cyrus Tang Medical Institute, The First Affiliated Hospital and Collaborative Innovation Center of Hematology, State Key Laboratory of Radiation Medicine and Protection, Suzhou Medical College, Soochow University, Key Laboratory of Thrombosis and Hemostasis, Ministry of Health, National Clinical Research Center for Hematological Diseases, Suzhou, People’s Republic of ChinaJiangsu Institute of Hematology, Cyrus Tang Medical Institute, The First Affiliated Hospital and Collaborative Innovation Center of Hematology, State Key Laboratory of Radiation Medicine and Protection, Suzhou Medical College, Soochow University, Key Laboratory of Thrombosis and Hemostasis, Ministry of Health, National Clinical Research Center for Hematological Diseases, Suzhou, People’s Republic of ChinaJiangsu Institute of Hematology, Cyrus Tang Medical Institute, The First Affiliated Hospital and Collaborative Innovation Center of Hematology, State Key Laboratory of Radiation Medicine and Protection, Suzhou Medical College, Soochow University, Key Laboratory of Thrombosis and Hemostasis, Ministry of Health, National Clinical Research Center for Hematological Diseases, Suzhou, People’s Republic of ChinaHematology Department of the Second Hospital of Anhui Medical University, Hematologic Diseases Research Center of Anhui Medical University, Hefei, People’s Republic of ChinaJiangsu Institute of Hematology, Cyrus Tang Medical Institute, The First Affiliated Hospital and Collaborative Innovation Center of Hematology, State Key Laboratory of Radiation Medicine and Protection, Suzhou Medical College, Soochow University, Key Laboratory of Thrombosis and Hemostasis, Ministry of Health, National Clinical Research Center for Hematological Diseases, Suzhou, People’s Republic of ChinaABSTRACTObjectives Cyclic thrombocytopenia (CTP) is a rare blood disorder characterized by periodic fluctuations in platelet counts. CTP usually appears in pre-menopausal women, and these fluctuations of platelets are in phase with the menstrual cycle. CTP is a heterogeneous disease, and the pathogenic mechanism is still unclear. Therefore, it harbors great significance for exploring the association of fluctuations in platelet counts with hormonal-cycle.Materials Firstly, we washed human platelets from healthy volunteers following the Declaration of Helsinki. Flow cytometer was employed to measure the mitochondrial inner transmembrane potential (ΔΨm) depolarization, PS exposure, P-selectin expression, and GPIIb/IIIa activation in platelets. In addition, western blot detected the related protein expression. The corresponding assay kit measured the caspase-3 and PDE3A activity. Finally, flow cytometry determined mouse platelets labeled with calcein.Results We find a reverse relationship between the platelet count and serum estradiol (E2) level in a CTP patient. We demonstrated that E2 induces platelet apoptosis in vitro and platelet clearance in vivo. We further discovered that E2 activates phosphodiesterase 3A, which inhibits protein kinase A (PKA), leading to PKA-mediated platelet apoptosis. Activation of PKA protected platelets from E2-induced thrombocytopenia and elevated the number of mice circulatory platelets.Conclusions We find that E2 induces platelet apoptosis and clearance through PDE3A-mediated PKA inhibition. Activation of PKA rescues E2-induced thrombocytopenia in mice. Thus, our study reveals a pathogenesis of E2-related CTP and suggests promising therapeutic strategies for the disease.https://www.tandfonline.com/doi/10.1080/16078454.2023.2240140Cyclic thrombocytopeniaplateletapoptosisestradiolprotein kinase A |
| spellingShingle | Ningbo Pang Yingwei Li Kangxi Zhou Chunliang Liu Rong Yan Chenglin Sun Weiling Xiao Changgeng Ruan Zhimin Zhai Kesheng Dai Cyclic thrombocytopenia associated with estradiol: a case report Hematology Cyclic thrombocytopenia platelet apoptosis estradiol protein kinase A |
| title | Cyclic thrombocytopenia associated with estradiol: a case report |
| title_full | Cyclic thrombocytopenia associated with estradiol: a case report |
| title_fullStr | Cyclic thrombocytopenia associated with estradiol: a case report |
| title_full_unstemmed | Cyclic thrombocytopenia associated with estradiol: a case report |
| title_short | Cyclic thrombocytopenia associated with estradiol: a case report |
| title_sort | cyclic thrombocytopenia associated with estradiol a case report |
| topic | Cyclic thrombocytopenia platelet apoptosis estradiol protein kinase A |
| url | https://www.tandfonline.com/doi/10.1080/16078454.2023.2240140 |
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