Naringenin prevents NAFLD in the diet-induced C57BL/6J obesity model by regulating the intestinal barrier function and microbiota
Objectives: The intestinal immunity and the microbiota play an essential role in metabolic syndrome associated with obesity, for instance, non-alcoholic fatty liver disease (NAFLD). However, the effects of naringenin on diet-induced NAFLD and intestinal functions have not been reported. Methods: The...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2023-06-01
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| Series: | Journal of Functional Foods |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S1756464623001780 |
| _version_ | 1827941018518945792 |
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| author | Ruyang Yu Yinping Gu Lanyan Zheng Zhongjie Liu Yifei Bian |
| author_facet | Ruyang Yu Yinping Gu Lanyan Zheng Zhongjie Liu Yifei Bian |
| author_sort | Ruyang Yu |
| collection | DOAJ |
| description | Objectives: The intestinal immunity and the microbiota play an essential role in metabolic syndrome associated with obesity, for instance, non-alcoholic fatty liver disease (NAFLD). However, the effects of naringenin on diet-induced NAFLD and intestinal functions have not been reported. Methods: The effects of naringenin on NAFLD were assessed in C57BL/6J administrated with a high-fat diet (HFD) and high-fat diet with 0.1% naringenin (HFN). The ELISA, histopathology, and flow cytometry were conducted to assess the effects of naringenin on the liver and intestinal inflammation. The FITC-dextran, western blot, and real-time quantitative PCR were used to evaluate the effects of naringenin on intestinal permeability. The regulatory effects of naringenin on intestinal microbiota in NAFLD mice were detected by 16S rRNA sequencing. Results: Naringenin could curb weight gain in obese mice, reduce the content of triglyceride (TG) and total cholesterol (TC) in blood and liver, and even improve the steatosis of liver cells. Naringenin alleviated the rate of infiltration in dendritic cells and macrophages in colon tissues and inhibited inflammatory factors in the intestinal and liver tissues. Naringenin reduced the gut barrier permeability caused by HFD and increased the expression of tight junction proteins (TJs). Furthermore, naringenin significantly improved the cecal microbiota disorder by enhancing the growth of salutary symbiotic bacteria and restraining the colonization of pathogenic bacteria. Conclusion: Naringenin could relieve metabolic disorders caused by HFD and improve gut barrier functions, thereby inhibiting the migration of intestinal microorganisms and products via the gut-liver axis, thus preventing NAFLD. |
| first_indexed | 2024-03-13T09:29:46Z |
| format | Article |
| id | doaj.art-ffe4beba8cdd4cdfb31c72b23e563d07 |
| institution | Directory Open Access Journal |
| issn | 1756-4646 |
| language | English |
| last_indexed | 2024-03-13T09:29:46Z |
| publishDate | 2023-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Functional Foods |
| spelling | doaj.art-ffe4beba8cdd4cdfb31c72b23e563d072023-05-26T04:21:16ZengElsevierJournal of Functional Foods1756-46462023-06-01105105578Naringenin prevents NAFLD in the diet-induced C57BL/6J obesity model by regulating the intestinal barrier function and microbiotaRuyang Yu0Yinping Gu1Lanyan Zheng2Zhongjie Liu3Yifei Bian4College of Veterinary Medicine, China Agricultural University, Yuan Ming Yuan West Road No. 2, Haidian District 100193, PR ChinaCollege of Veterinary Medicine, China Agricultural University, Yuan Ming Yuan West Road No. 2, Haidian District 100193, PR ChinaCollege of Veterinary Medicine, China Agricultural University, Yuan Ming Yuan West Road No. 2, Haidian District 100193, PR ChinaCollege of Veterinary Medicine, China Agricultural University, Yuan Ming Yuan West Road No. 2, Haidian District 100193, PR China; Corresponding authors.Innovation Research Institute of Traditional Chinese Medicine, Shandong University of Traditional Chinese Medicine, Changqing District, Ji’nan, PR China; Corresponding authors.Objectives: The intestinal immunity and the microbiota play an essential role in metabolic syndrome associated with obesity, for instance, non-alcoholic fatty liver disease (NAFLD). However, the effects of naringenin on diet-induced NAFLD and intestinal functions have not been reported. Methods: The effects of naringenin on NAFLD were assessed in C57BL/6J administrated with a high-fat diet (HFD) and high-fat diet with 0.1% naringenin (HFN). The ELISA, histopathology, and flow cytometry were conducted to assess the effects of naringenin on the liver and intestinal inflammation. The FITC-dextran, western blot, and real-time quantitative PCR were used to evaluate the effects of naringenin on intestinal permeability. The regulatory effects of naringenin on intestinal microbiota in NAFLD mice were detected by 16S rRNA sequencing. Results: Naringenin could curb weight gain in obese mice, reduce the content of triglyceride (TG) and total cholesterol (TC) in blood and liver, and even improve the steatosis of liver cells. Naringenin alleviated the rate of infiltration in dendritic cells and macrophages in colon tissues and inhibited inflammatory factors in the intestinal and liver tissues. Naringenin reduced the gut barrier permeability caused by HFD and increased the expression of tight junction proteins (TJs). Furthermore, naringenin significantly improved the cecal microbiota disorder by enhancing the growth of salutary symbiotic bacteria and restraining the colonization of pathogenic bacteria. Conclusion: Naringenin could relieve metabolic disorders caused by HFD and improve gut barrier functions, thereby inhibiting the migration of intestinal microorganisms and products via the gut-liver axis, thus preventing NAFLD.http://www.sciencedirect.com/science/article/pii/S1756464623001780NAFLDNaringeninHFDGut barrierIntestinal microbiota |
| spellingShingle | Ruyang Yu Yinping Gu Lanyan Zheng Zhongjie Liu Yifei Bian Naringenin prevents NAFLD in the diet-induced C57BL/6J obesity model by regulating the intestinal barrier function and microbiota Journal of Functional Foods NAFLD Naringenin HFD Gut barrier Intestinal microbiota |
| title | Naringenin prevents NAFLD in the diet-induced C57BL/6J obesity model by regulating the intestinal barrier function and microbiota |
| title_full | Naringenin prevents NAFLD in the diet-induced C57BL/6J obesity model by regulating the intestinal barrier function and microbiota |
| title_fullStr | Naringenin prevents NAFLD in the diet-induced C57BL/6J obesity model by regulating the intestinal barrier function and microbiota |
| title_full_unstemmed | Naringenin prevents NAFLD in the diet-induced C57BL/6J obesity model by regulating the intestinal barrier function and microbiota |
| title_short | Naringenin prevents NAFLD in the diet-induced C57BL/6J obesity model by regulating the intestinal barrier function and microbiota |
| title_sort | naringenin prevents nafld in the diet induced c57bl 6j obesity model by regulating the intestinal barrier function and microbiota |
| topic | NAFLD Naringenin HFD Gut barrier Intestinal microbiota |
| url | http://www.sciencedirect.com/science/article/pii/S1756464623001780 |
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