Differentially Expressed Genes in Osteomyelitis Induced by Staphylococcus aureus Infection
Osteomyelitis (OM) is a complicated and serious disease and its underlying molecular signatures of disease initiation and progression remain unclear. Staphylococcus aureus (S. aureus) is the most common causative agent of OM. Previous study of Banchereau et al. has established a link between whole b...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2018-05-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/article/10.3389/fmicb.2018.01093/full |
| _version_ | 1811274982537297920 |
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| author | Peisheng Chen Peisheng Chen Zilong Yao Zilong Yao Ganming Deng Ganming Deng Yilong Hou Yilong Hou Siwei Chen Siwei Chen Yanjun Hu Bin Yu Bin Yu |
| author_facet | Peisheng Chen Peisheng Chen Zilong Yao Zilong Yao Ganming Deng Ganming Deng Yilong Hou Yilong Hou Siwei Chen Siwei Chen Yanjun Hu Bin Yu Bin Yu |
| author_sort | Peisheng Chen |
| collection | DOAJ |
| description | Osteomyelitis (OM) is a complicated and serious disease and its underlying molecular signatures of disease initiation and progression remain unclear. Staphylococcus aureus (S. aureus) is the most common causative agent of OM. Previous study of Banchereau et al. has established a link between whole blood transcription profiles and clinical manifestations in patients infected with S. aureus. However, the differentially expressed genes (DEGs) in OM induced by S. aureus infection have not been intensively investigated. In this study, we downloaded the gene expression profile dataset GSE30119 from Gene Expression Omnibus, and performed bioinformatic analysis to identify DEGs in S. aureus infection induced OM from the transcriptional level. The study consisted of 143 whole blood samples, including 44 healthy controls, 42 OM-free, and 57 OM infection patients. A total of 209 S. aureus infection-related genes (SARGs) and 377 OM-related genes (OMRGs) were identified. The SARGs were primarily involved in the immune response by GO functional and pathway enrichment analysis. Several proteins adhere to neutrophil extracellular traps may be critical for the immune response to the process of S. aureus infection. By contrast, the OMRGs differ from the SARGs. The OMRGs were enriched in transmembrane signaling receptor and calcium channel activity, cilium morphogenesis, chromatin silencing, even multicellular organism development. Several key proteins, including PHLPP2 and EGF, were hub nodes in protein–protein interaction network of the OMRGs. In addition, alcoholism, systemic lupus erythematosus and proteoglycans in cancer were the top pathways influenced by the OMRGs associated with OM. Thus, this study has further explored the DEGs and their biological functions associated with S. aureus infection and OM, comparing with the previous study, and may light the further insight into the underlying molecular mechanisms and the potential critical biomarkers in OM development. |
| first_indexed | 2024-04-12T23:28:53Z |
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| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-04-12T23:28:53Z |
| publishDate | 2018-05-01 |
| publisher | Frontiers Media S.A. |
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| series | Frontiers in Microbiology |
| spelling | doaj.art-ffe5fadb3f14418f866a2afa629b07522022-12-22T03:12:20ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2018-05-01910.3389/fmicb.2018.01093353782Differentially Expressed Genes in Osteomyelitis Induced by Staphylococcus aureus InfectionPeisheng Chen0Peisheng Chen1Zilong Yao2Zilong Yao3Ganming Deng4Ganming Deng5Yilong Hou6Yilong Hou7Siwei Chen8Siwei Chen9Yanjun Hu10Bin Yu11Bin Yu12Department of Orthopaedics and Traumatology, Nanfang Hospital, Southern Medical University, Guangzhou, ChinaGuangdong Provincial Key Laboratory of Bone and Cartilage Regenerative Medicine, Nanfang Hospital, Southern Medical University, Guangzhou, ChinaDepartment of Orthopaedics and Traumatology, Nanfang Hospital, Southern Medical University, Guangzhou, ChinaGuangdong Provincial Key Laboratory of Bone and Cartilage Regenerative Medicine, Nanfang Hospital, Southern Medical University, Guangzhou, ChinaDepartment of Orthopaedics and Traumatology, Nanfang Hospital, Southern Medical University, Guangzhou, ChinaGuangdong Provincial Key Laboratory of Bone and Cartilage Regenerative Medicine, Nanfang Hospital, Southern Medical University, Guangzhou, ChinaDepartment of Orthopaedics and Traumatology, Nanfang Hospital, Southern Medical University, Guangzhou, ChinaGuangdong Provincial Key Laboratory of Bone and Cartilage Regenerative Medicine, Nanfang Hospital, Southern Medical University, Guangzhou, ChinaDepartment of Orthopaedics and Traumatology, Nanfang Hospital, Southern Medical University, Guangzhou, ChinaGuangdong Provincial Key Laboratory of Bone and Cartilage Regenerative Medicine, Nanfang Hospital, Southern Medical University, Guangzhou, ChinaDepartment of Orthopaedics and Traumatology, Nanfang Hospital, Southern Medical University, Guangzhou, ChinaDepartment of Orthopaedics and Traumatology, Nanfang Hospital, Southern Medical University, Guangzhou, ChinaGuangdong Provincial Key Laboratory of Bone and Cartilage Regenerative Medicine, Nanfang Hospital, Southern Medical University, Guangzhou, ChinaOsteomyelitis (OM) is a complicated and serious disease and its underlying molecular signatures of disease initiation and progression remain unclear. Staphylococcus aureus (S. aureus) is the most common causative agent of OM. Previous study of Banchereau et al. has established a link between whole blood transcription profiles and clinical manifestations in patients infected with S. aureus. However, the differentially expressed genes (DEGs) in OM induced by S. aureus infection have not been intensively investigated. In this study, we downloaded the gene expression profile dataset GSE30119 from Gene Expression Omnibus, and performed bioinformatic analysis to identify DEGs in S. aureus infection induced OM from the transcriptional level. The study consisted of 143 whole blood samples, including 44 healthy controls, 42 OM-free, and 57 OM infection patients. A total of 209 S. aureus infection-related genes (SARGs) and 377 OM-related genes (OMRGs) were identified. The SARGs were primarily involved in the immune response by GO functional and pathway enrichment analysis. Several proteins adhere to neutrophil extracellular traps may be critical for the immune response to the process of S. aureus infection. By contrast, the OMRGs differ from the SARGs. The OMRGs were enriched in transmembrane signaling receptor and calcium channel activity, cilium morphogenesis, chromatin silencing, even multicellular organism development. Several key proteins, including PHLPP2 and EGF, were hub nodes in protein–protein interaction network of the OMRGs. In addition, alcoholism, systemic lupus erythematosus and proteoglycans in cancer were the top pathways influenced by the OMRGs associated with OM. Thus, this study has further explored the DEGs and their biological functions associated with S. aureus infection and OM, comparing with the previous study, and may light the further insight into the underlying molecular mechanisms and the potential critical biomarkers in OM development.https://www.frontiersin.org/article/10.3389/fmicb.2018.01093/fullosteomyelitis (OM)Staphylococcus aureus (S. aureus)candidate biomarkersbioinformatic analysisdifferentially expressed genes (DEGs) |
| spellingShingle | Peisheng Chen Peisheng Chen Zilong Yao Zilong Yao Ganming Deng Ganming Deng Yilong Hou Yilong Hou Siwei Chen Siwei Chen Yanjun Hu Bin Yu Bin Yu Differentially Expressed Genes in Osteomyelitis Induced by Staphylococcus aureus Infection Frontiers in Microbiology osteomyelitis (OM) Staphylococcus aureus (S. aureus) candidate biomarkers bioinformatic analysis differentially expressed genes (DEGs) |
| title | Differentially Expressed Genes in Osteomyelitis Induced by Staphylococcus aureus Infection |
| title_full | Differentially Expressed Genes in Osteomyelitis Induced by Staphylococcus aureus Infection |
| title_fullStr | Differentially Expressed Genes in Osteomyelitis Induced by Staphylococcus aureus Infection |
| title_full_unstemmed | Differentially Expressed Genes in Osteomyelitis Induced by Staphylococcus aureus Infection |
| title_short | Differentially Expressed Genes in Osteomyelitis Induced by Staphylococcus aureus Infection |
| title_sort | differentially expressed genes in osteomyelitis induced by staphylococcus aureus infection |
| topic | osteomyelitis (OM) Staphylococcus aureus (S. aureus) candidate biomarkers bioinformatic analysis differentially expressed genes (DEGs) |
| url | https://www.frontiersin.org/article/10.3389/fmicb.2018.01093/full |
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