Mutating alfalfa COUMARATE 3-HYDROXYLASE using multiplex CRISPR/Cas9 leads to reduced lignin deposition and improved forage quality

Mutating alfalfa COUMARATE 3-HYDROXYLASE using multiplex CRISPR/Cas9 leads to reduced lignin deposition and improved forage quality

Alfalfa (Medicago sativa L.) forage quality is adversely affected by lignin deposition in cell walls at advanced maturity stages. Reducing lignin content through RNA interference or antisense approaches has been shown to improve alfalfa forage quality and digestibility. We employed a multiplex CRISP...

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Bibliographic Details
Main Authors: Tezera W. Wolabu, Kashif Mahmood, Fang Chen, Ivone Torres-Jerez, Michael Udvardi, Million Tadege, Lili Cong, Zengyu Wang, Jiangqi Wen
Format: Article
Language:English
Published: Frontiers Media S.A. 2024-03-01
Series:Frontiers in Plant Science
Subjects:
CRISPR/Cas9
gene editing
lignin composition
alfalfa
forage quality
MsC3H
Online Access:https://www.frontiersin.org/articles/10.3389/fpls.2024.1363182/full

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