Rheological properties of corn stover slurries during fermentation by Clostridium thermocellum
Abstract Background Milling during fermentation, termed cotreatment, has recently been proposed as an alternative to thermochemical pretreatment as a means to increase the accessibility of lignocellulosic biomass to biological attack. A central premise of this approach is that partial solubilization...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2018-09-01
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| Series: | Biotechnology for Biofuels |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s13068-018-1248-z |
| _version_ | 1811336732980805632 |
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| author | Sanchari Ghosh Evert K. Holwerda Robert S. Worthen Lee R. Lynd Brenden P. Epps |
| author_facet | Sanchari Ghosh Evert K. Holwerda Robert S. Worthen Lee R. Lynd Brenden P. Epps |
| author_sort | Sanchari Ghosh |
| collection | DOAJ |
| description | Abstract Background Milling during fermentation, termed cotreatment, has recently been proposed as an alternative to thermochemical pretreatment as a means to increase the accessibility of lignocellulosic biomass to biological attack. A central premise of this approach is that partial solubilization of biomass changes the slurry’s physical properties such that milling becomes more impactful and more feasible. A key uncertainty is the energy required to mill partially fermented biomass. To inform both of these issues, we report rheological characterization of small-particle, corn stover slurries undergoing fermentation by Clostridium thermocellum. Results Fermented and unfermented corn stover slurries were found to be shear-thinning and well described by a power law model with an exponent of 0.10. Plastic viscosity of a slurry, initially at 16 wt.% insoluble solids, decreased as a result of fermentation by a factor of 2000, with the first eightfold reduction occurring in the first 10% of carbohydrate conversion. Large amplitude oscillatory shear experiments revealed only minor changes to the slurry’s rheological fingerprint as a result of fermentation, with the notable change being a reduction in the critical strain amplitude needed for the onset of nonlinearity. All slurries were found to be elastoviscoplastic, with the elastic/viscous crossover at roughly 100% strain amplitude. Conclusions Whereas prior biomass rheology studies have involved pretreated feedstocks and solubilization mediated by fungal cellulase, we report results for feedstocks with no pretreatment other than autoclaving and for solubilization mediated by C. thermocellum. As observed in prior studies, C. thermocellum fermentation results in a dramatic decrease in viscosity. The magnitude of this decrease, however, is much larger starting with unpretreated feedstock than previously reported for pretreated feedstocks. LAOS measurements provide a detailed picture of the rheological fingerprint of the material. Viscosity measurements confirm the hypothesis that the physical character of corn stover slurries changes dramatically during fermentation by C. thermocellum, and indicate that the energy expended on overcoming slurry viscosity will be far less for partially fermented corn stover than for unfermented corn stover. |
| first_indexed | 2024-04-13T17:44:46Z |
| format | Article |
| id | doaj.art-ffeba3979d8d4a428ded290cc1fbe423 |
| institution | Directory Open Access Journal |
| issn | 1754-6834 |
| language | English |
| last_indexed | 2024-04-13T17:44:46Z |
| publishDate | 2018-09-01 |
| publisher | BMC |
| record_format | Article |
| series | Biotechnology for Biofuels |
| spelling | doaj.art-ffeba3979d8d4a428ded290cc1fbe4232022-12-22T02:37:04ZengBMCBiotechnology for Biofuels1754-68342018-09-0111111210.1186/s13068-018-1248-zRheological properties of corn stover slurries during fermentation by Clostridium thermocellumSanchari Ghosh0Evert K. Holwerda1Robert S. Worthen2Lee R. Lynd3Brenden P. Epps4Thayer School of Engineering, Dartmouth CollegeThayer School of Engineering, Dartmouth CollegeThayer School of Engineering, Dartmouth CollegeThayer School of Engineering, Dartmouth CollegeThayer School of Engineering, Dartmouth CollegeAbstract Background Milling during fermentation, termed cotreatment, has recently been proposed as an alternative to thermochemical pretreatment as a means to increase the accessibility of lignocellulosic biomass to biological attack. A central premise of this approach is that partial solubilization of biomass changes the slurry’s physical properties such that milling becomes more impactful and more feasible. A key uncertainty is the energy required to mill partially fermented biomass. To inform both of these issues, we report rheological characterization of small-particle, corn stover slurries undergoing fermentation by Clostridium thermocellum. Results Fermented and unfermented corn stover slurries were found to be shear-thinning and well described by a power law model with an exponent of 0.10. Plastic viscosity of a slurry, initially at 16 wt.% insoluble solids, decreased as a result of fermentation by a factor of 2000, with the first eightfold reduction occurring in the first 10% of carbohydrate conversion. Large amplitude oscillatory shear experiments revealed only minor changes to the slurry’s rheological fingerprint as a result of fermentation, with the notable change being a reduction in the critical strain amplitude needed for the onset of nonlinearity. All slurries were found to be elastoviscoplastic, with the elastic/viscous crossover at roughly 100% strain amplitude. Conclusions Whereas prior biomass rheology studies have involved pretreated feedstocks and solubilization mediated by fungal cellulase, we report results for feedstocks with no pretreatment other than autoclaving and for solubilization mediated by C. thermocellum. As observed in prior studies, C. thermocellum fermentation results in a dramatic decrease in viscosity. The magnitude of this decrease, however, is much larger starting with unpretreated feedstock than previously reported for pretreated feedstocks. LAOS measurements provide a detailed picture of the rheological fingerprint of the material. Viscosity measurements confirm the hypothesis that the physical character of corn stover slurries changes dramatically during fermentation by C. thermocellum, and indicate that the energy expended on overcoming slurry viscosity will be far less for partially fermented corn stover than for unfermented corn stover.http://link.springer.com/article/10.1186/s13068-018-1248-zBiomassCorn stoverRheologySlurry viscosityLarge amplitude oscillatory shearCotreatment |
| spellingShingle | Sanchari Ghosh Evert K. Holwerda Robert S. Worthen Lee R. Lynd Brenden P. Epps Rheological properties of corn stover slurries during fermentation by Clostridium thermocellum Biotechnology for Biofuels Biomass Corn stover Rheology Slurry viscosity Large amplitude oscillatory shear Cotreatment |
| title | Rheological properties of corn stover slurries during fermentation by Clostridium thermocellum |
| title_full | Rheological properties of corn stover slurries during fermentation by Clostridium thermocellum |
| title_fullStr | Rheological properties of corn stover slurries during fermentation by Clostridium thermocellum |
| title_full_unstemmed | Rheological properties of corn stover slurries during fermentation by Clostridium thermocellum |
| title_short | Rheological properties of corn stover slurries during fermentation by Clostridium thermocellum |
| title_sort | rheological properties of corn stover slurries during fermentation by clostridium thermocellum |
| topic | Biomass Corn stover Rheology Slurry viscosity Large amplitude oscillatory shear Cotreatment |
| url | http://link.springer.com/article/10.1186/s13068-018-1248-z |
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