Glycosylphosphatidylinositol Anchors from Galactomannan and GPI-Anchored Protein Are Synthesized by Distinct Pathways in Aspergillus fumigatus
Glycosylphosphatidylinositols (GPIs) are lipid anchors allowing the exposure of proteins at the outer layer of the plasma membrane. In fungi, a number of GPI-anchored proteins (GPI-APs) are involved in the remodeling of the cell wall polymers. GPIs follow a specific biosynthetic pathway in the endop...
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| Format: | Article |
| Language: | English |
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MDPI AG
2018-02-01
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| Series: | Journal of Fungi |
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| Online Access: | http://www.mdpi.com/2309-608X/4/1/19 |
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| author | Jizhou Li Isabelle Mouyna Christine Henry Frédérique Moyrand Christian Malosse Julia Chamot-Rooke Guilhem Janbon Jean-Paul Latgé Thierry Fontaine |
| author_facet | Jizhou Li Isabelle Mouyna Christine Henry Frédérique Moyrand Christian Malosse Julia Chamot-Rooke Guilhem Janbon Jean-Paul Latgé Thierry Fontaine |
| author_sort | Jizhou Li |
| collection | DOAJ |
| description | Glycosylphosphatidylinositols (GPIs) are lipid anchors allowing the exposure of proteins at the outer layer of the plasma membrane. In fungi, a number of GPI-anchored proteins (GPI-APs) are involved in the remodeling of the cell wall polymers. GPIs follow a specific biosynthetic pathway in the endoplasmic reticulum. After the transfer of the protein onto the GPI-anchor, a lipid remodeling occurs to substitute the diacylglycerol moiety by a ceramide. In addition to GPI-APs, A. fumigatus produces a GPI-anchored polysaccharide, the galactomannan (GM), that remains unique in the fungal kingdom. To investigate the role of the GPI pathway in the biosynthesis of the GM and cell wall organization, the deletion of PER1—coding for a phospholipase required for the first step of the GPI lipid remodeling—was undertaken. Biochemical characterization of the GPI-anchor isolated from GPI-APs showed that the PER1 deficient mutant produced a lipid anchor with a diacylglycerol. The absence of a ceramide on GPI-anchors in the Δper1 mutant led to a mislocation of GPI-APs and to an alteration of the composition of the cell wall alkali-insoluble fraction. On the other hand, the GM isolated from the Δper1 mutant membranes possesses a ceramide moiety as the parental strain, showing that GPI anchor of the GM follow a distinct unknown biosynthetic pathway. |
| first_indexed | 2024-12-21T17:14:11Z |
| format | Article |
| id | doaj.art-ffebfd7c5c3b4571909bc85c444dd97d |
| institution | Directory Open Access Journal |
| issn | 2309-608X |
| language | English |
| last_indexed | 2024-12-21T17:14:11Z |
| publishDate | 2018-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Journal of Fungi |
| spelling | doaj.art-ffebfd7c5c3b4571909bc85c444dd97d2022-12-21T18:56:21ZengMDPI AGJournal of Fungi2309-608X2018-02-01411910.3390/jof4010019jof4010019Glycosylphosphatidylinositol Anchors from Galactomannan and GPI-Anchored Protein Are Synthesized by Distinct Pathways in Aspergillus fumigatusJizhou Li0Isabelle Mouyna1Christine Henry2Frédérique Moyrand3Christian Malosse4Julia Chamot-Rooke5Guilhem Janbon6Jean-Paul Latgé7Thierry Fontaine8Unité des Aspergillus, 25 rue du Docteur Roux, Institut Pasteur, 25 rue du Docteur Roux, 75015 Paris, FranceUnité des Aspergillus, 25 rue du Docteur Roux, Institut Pasteur, 25 rue du Docteur Roux, 75015 Paris, FranceUnité des Aspergillus, 25 rue du Docteur Roux, Institut Pasteur, 25 rue du Docteur Roux, 75015 Paris, FranceUnité de Biologie des ARN des Pathogènes Fongiques, Institut Pasteur, 25 rue du Docteur Roux, 75015 Paris, FranceUnité de Spectrométrie de Masse pour la Biologie, Institut Pasteur, CNRS USR 2000, 28 rue du Docteur Roux, 75015 Paris, FranceUnité de Spectrométrie de Masse pour la Biologie, Institut Pasteur, CNRS USR 2000, 28 rue du Docteur Roux, 75015 Paris, FranceUnité de Biologie des ARN des Pathogènes Fongiques, Institut Pasteur, 25 rue du Docteur Roux, 75015 Paris, FranceUnité des Aspergillus, 25 rue du Docteur Roux, Institut Pasteur, 25 rue du Docteur Roux, 75015 Paris, FranceUnité des Aspergillus, 25 rue du Docteur Roux, Institut Pasteur, 25 rue du Docteur Roux, 75015 Paris, FranceGlycosylphosphatidylinositols (GPIs) are lipid anchors allowing the exposure of proteins at the outer layer of the plasma membrane. In fungi, a number of GPI-anchored proteins (GPI-APs) are involved in the remodeling of the cell wall polymers. GPIs follow a specific biosynthetic pathway in the endoplasmic reticulum. After the transfer of the protein onto the GPI-anchor, a lipid remodeling occurs to substitute the diacylglycerol moiety by a ceramide. In addition to GPI-APs, A. fumigatus produces a GPI-anchored polysaccharide, the galactomannan (GM), that remains unique in the fungal kingdom. To investigate the role of the GPI pathway in the biosynthesis of the GM and cell wall organization, the deletion of PER1—coding for a phospholipase required for the first step of the GPI lipid remodeling—was undertaken. Biochemical characterization of the GPI-anchor isolated from GPI-APs showed that the PER1 deficient mutant produced a lipid anchor with a diacylglycerol. The absence of a ceramide on GPI-anchors in the Δper1 mutant led to a mislocation of GPI-APs and to an alteration of the composition of the cell wall alkali-insoluble fraction. On the other hand, the GM isolated from the Δper1 mutant membranes possesses a ceramide moiety as the parental strain, showing that GPI anchor of the GM follow a distinct unknown biosynthetic pathway.http://www.mdpi.com/2309-608X/4/1/19glycosylphosphatidylinositolAspergillus fumigatusPER1cell wallgalactomannan |
| spellingShingle | Jizhou Li Isabelle Mouyna Christine Henry Frédérique Moyrand Christian Malosse Julia Chamot-Rooke Guilhem Janbon Jean-Paul Latgé Thierry Fontaine Glycosylphosphatidylinositol Anchors from Galactomannan and GPI-Anchored Protein Are Synthesized by Distinct Pathways in Aspergillus fumigatus Journal of Fungi glycosylphosphatidylinositol Aspergillus fumigatus PER1 cell wall galactomannan |
| title | Glycosylphosphatidylinositol Anchors from Galactomannan and GPI-Anchored Protein Are Synthesized by Distinct Pathways in Aspergillus fumigatus |
| title_full | Glycosylphosphatidylinositol Anchors from Galactomannan and GPI-Anchored Protein Are Synthesized by Distinct Pathways in Aspergillus fumigatus |
| title_fullStr | Glycosylphosphatidylinositol Anchors from Galactomannan and GPI-Anchored Protein Are Synthesized by Distinct Pathways in Aspergillus fumigatus |
| title_full_unstemmed | Glycosylphosphatidylinositol Anchors from Galactomannan and GPI-Anchored Protein Are Synthesized by Distinct Pathways in Aspergillus fumigatus |
| title_short | Glycosylphosphatidylinositol Anchors from Galactomannan and GPI-Anchored Protein Are Synthesized by Distinct Pathways in Aspergillus fumigatus |
| title_sort | glycosylphosphatidylinositol anchors from galactomannan and gpi anchored protein are synthesized by distinct pathways in aspergillus fumigatus |
| topic | glycosylphosphatidylinositol Aspergillus fumigatus PER1 cell wall galactomannan |
| url | http://www.mdpi.com/2309-608X/4/1/19 |
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