Scrutinizing pharmacological efficiency for Acacia auriculiformis by experimental and computational approach
Abstract Background The study sought to investigate the biological efficacy of methanol leave extract of Acacia auriculiformis (MEAA) via in vitro, in vivo, in silico approaches. The in vitro cytotoxicity was evaluated through brine shrimp lethality assay, and anti-inflammatory activity was determin...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
SpringerOpen
2021-03-01
|
| Series: | Future Journal of Pharmaceutical Sciences |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s43094-021-00221-7 |
| _version_ | 1818352583254736896 |
|---|---|
| author | Shafinaz Nur Md. Mohotasin Hossain Nadia Islam Abu Montakim Tareq Nujhat Binte Hanif Riniara Khatun Mohammed Abu Sayeed |
| author_facet | Shafinaz Nur Md. Mohotasin Hossain Nadia Islam Abu Montakim Tareq Nujhat Binte Hanif Riniara Khatun Mohammed Abu Sayeed |
| author_sort | Shafinaz Nur |
| collection | DOAJ |
| description | Abstract Background The study sought to investigate the biological efficacy of methanol leave extract of Acacia auriculiformis (MEAA) via in vitro, in vivo, in silico approaches. The in vitro cytotoxicity was evaluated through brine shrimp lethality assay, and anti-inflammatory activity was determined by membrane stabilisation and protein denaturation methods (BSA and egg albumin). The in vivo antipyretic activity was examined via Brewer’s yeast induced pyrexia model. Results A. auriculiformis extract unveiled moderate cytotoxicity with significant anti-inflammatory efficacy (p < 0.001) compared to standard drug. This extract also exhibited dose-dependent time of paralysis and death for the worm (p < 0.001) in the anthelmintic test which was directly proportional to employed concentrations. A notable percentage of clot lysis effect (36.42 ± 1.95%, p < 0.001) was also observed for MEAA in human blood compared to control. However, this extract significantly (p < 0.05) reduced fever in a dose-dependent manner during the antipyretic experiment. Besides, in computer-aided investigations, two compounds (2,4-ditert-butylphenol and 3-hydroxy-β-damascone) revealed the best binding interaction with six proteins for cytotoxicity, inflammation, helminthic, thrombolytic and pyretic effect. Moreover, these two compounds satisfy Lipinski’s ‘Rule of Five’ and revealed drug-likeness profiles in the toxicological study. Conclusions These findings disclosed that methanol leaves extract of A. auriculiformis might be a potent source for anti-inflammatory, anti-helminthic, thrombolytic and antipyretic agents. |
| first_indexed | 2024-12-13T18:55:56Z |
| format | Article |
| id | doaj.art-ffec4111e5b14bb58b8717ed2fcc04d1 |
| institution | Directory Open Access Journal |
| issn | 2314-7253 |
| language | English |
| last_indexed | 2024-12-13T18:55:56Z |
| publishDate | 2021-03-01 |
| publisher | SpringerOpen |
| record_format | Article |
| series | Future Journal of Pharmaceutical Sciences |
| spelling | doaj.art-ffec4111e5b14bb58b8717ed2fcc04d12022-12-21T23:34:48ZengSpringerOpenFuture Journal of Pharmaceutical Sciences2314-72532021-03-017111510.1186/s43094-021-00221-7Scrutinizing pharmacological efficiency for Acacia auriculiformis by experimental and computational approachShafinaz Nur0Md. Mohotasin Hossain1Nadia Islam2Abu Montakim Tareq3Nujhat Binte Hanif4Riniara Khatun5Mohammed Abu Sayeed6Department of Pharmacy, International Islamic University ChittagongDepartment of Pharmacy, International Islamic University ChittagongDepartment of Pharmacy, International Islamic University ChittagongDepartment of Pharmacy, International Islamic University ChittagongDepartment of Pharmacy, International Islamic University ChittagongDepartment of Pharmacy, International Islamic University ChittagongDepartment of Pharmacy, International Islamic University ChittagongAbstract Background The study sought to investigate the biological efficacy of methanol leave extract of Acacia auriculiformis (MEAA) via in vitro, in vivo, in silico approaches. The in vitro cytotoxicity was evaluated through brine shrimp lethality assay, and anti-inflammatory activity was determined by membrane stabilisation and protein denaturation methods (BSA and egg albumin). The in vivo antipyretic activity was examined via Brewer’s yeast induced pyrexia model. Results A. auriculiformis extract unveiled moderate cytotoxicity with significant anti-inflammatory efficacy (p < 0.001) compared to standard drug. This extract also exhibited dose-dependent time of paralysis and death for the worm (p < 0.001) in the anthelmintic test which was directly proportional to employed concentrations. A notable percentage of clot lysis effect (36.42 ± 1.95%, p < 0.001) was also observed for MEAA in human blood compared to control. However, this extract significantly (p < 0.05) reduced fever in a dose-dependent manner during the antipyretic experiment. Besides, in computer-aided investigations, two compounds (2,4-ditert-butylphenol and 3-hydroxy-β-damascone) revealed the best binding interaction with six proteins for cytotoxicity, inflammation, helminthic, thrombolytic and pyretic effect. Moreover, these two compounds satisfy Lipinski’s ‘Rule of Five’ and revealed drug-likeness profiles in the toxicological study. Conclusions These findings disclosed that methanol leaves extract of A. auriculiformis might be a potent source for anti-inflammatory, anti-helminthic, thrombolytic and antipyretic agents.https://doi.org/10.1186/s43094-021-00221-7Acacia auriculiformisAnti-inflammatoryAnthelminticThrombolyticAntipyretic |
| spellingShingle | Shafinaz Nur Md. Mohotasin Hossain Nadia Islam Abu Montakim Tareq Nujhat Binte Hanif Riniara Khatun Mohammed Abu Sayeed Scrutinizing pharmacological efficiency for Acacia auriculiformis by experimental and computational approach Future Journal of Pharmaceutical Sciences Acacia auriculiformis Anti-inflammatory Anthelmintic Thrombolytic Antipyretic |
| title | Scrutinizing pharmacological efficiency for Acacia auriculiformis by experimental and computational approach |
| title_full | Scrutinizing pharmacological efficiency for Acacia auriculiformis by experimental and computational approach |
| title_fullStr | Scrutinizing pharmacological efficiency for Acacia auriculiformis by experimental and computational approach |
| title_full_unstemmed | Scrutinizing pharmacological efficiency for Acacia auriculiformis by experimental and computational approach |
| title_short | Scrutinizing pharmacological efficiency for Acacia auriculiformis by experimental and computational approach |
| title_sort | scrutinizing pharmacological efficiency for acacia auriculiformis by experimental and computational approach |
| topic | Acacia auriculiformis Anti-inflammatory Anthelmintic Thrombolytic Antipyretic |
| url | https://doi.org/10.1186/s43094-021-00221-7 |
| work_keys_str_mv | AT shafinaznur scrutinizingpharmacologicalefficiencyforacaciaauriculiformisbyexperimentalandcomputationalapproach AT mdmohotasinhossain scrutinizingpharmacologicalefficiencyforacaciaauriculiformisbyexperimentalandcomputationalapproach AT nadiaislam scrutinizingpharmacologicalefficiencyforacaciaauriculiformisbyexperimentalandcomputationalapproach AT abumontakimtareq scrutinizingpharmacologicalefficiencyforacaciaauriculiformisbyexperimentalandcomputationalapproach AT nujhatbintehanif scrutinizingpharmacologicalefficiencyforacaciaauriculiformisbyexperimentalandcomputationalapproach AT riniarakhatun scrutinizingpharmacologicalefficiencyforacaciaauriculiformisbyexperimentalandcomputationalapproach AT mohammedabusayeed scrutinizingpharmacologicalefficiencyforacaciaauriculiformisbyexperimentalandcomputationalapproach |