Toxicogenomics of the Freshwater Oligochaete, <i>Tubifex tubifex</i> (<i>Annelida</i>, <i>Clitellata</i>), in Acute Water-Only Exposure to Arsenic
A toxicogenomic approach was used for toxicity evaluation of arsenic in the aquatic environment, and differential gene expression was investigated from 24 h and 96 h water-only acute toxicity tests with the aquatic oligochaete, <i>Tubifex tubifex</i> (<i>Annelida</i>, <i&g...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2024-03-01
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| Series: | International Journal of Molecular Sciences |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1422-0067/25/6/3382 |
| Summary: | A toxicogenomic approach was used for toxicity evaluation of arsenic in the aquatic environment, and differential gene expression was investigated from 24 h and 96 h water-only acute toxicity tests with the aquatic oligochaete, <i>Tubifex tubifex</i> (<i>Annelida</i>, <i>Clitellata</i>). Several toxicological endpoints (survival and autotomy) of the oligochaete and tissue residues were measured, and dose-response modelling of gene expression data was studied. A reference transcriptome of the aquatic oligochaete, <i>T. tubifex</i>, was reconstructed for the first time, and genes related to cell stress response (<i>Hsc70</i>, <i>Hsp10</i>, <i>Hsp60</i>, and <i>Hsp83</i>), energy metabolism (<i>COX1</i>), oxidative stress (<i>Cat</i>, <i>GSR</i>, and <i>MnSOD</i>), and the genes involved in the homeostasis of organisms (<i>CaM</i>, <i>RpS13</i>, and <i>UBE2</i>) were identified and characterised. The potential use of the genes identified for risk assessment in freshwater ecosystems as early biomarkers of arsenic toxicity is discussed. |
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| ISSN: | 1661-6596 1422-0067 |