Toxicogenomics of the Freshwater Oligochaete, <i>Tubifex tubifex</i> (<i>Annelida</i>, <i>Clitellata</i>), in Acute Water-Only Exposure to Arsenic
A toxicogenomic approach was used for toxicity evaluation of arsenic in the aquatic environment, and differential gene expression was investigated from 24 h and 96 h water-only acute toxicity tests with the aquatic oligochaete, <i>Tubifex tubifex</i> (<i>Annelida</i>, <i&g...
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| Format: | Article |
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MDPI AG
2024-03-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/25/6/3382 |
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| author | Iñigo Moreno-Ocio Mónica Aquilino Lola Llorente Maite Martínez-Madrid Pilar Rodríguez Leire Méndez-Fernández Rosario Planelló |
| author_facet | Iñigo Moreno-Ocio Mónica Aquilino Lola Llorente Maite Martínez-Madrid Pilar Rodríguez Leire Méndez-Fernández Rosario Planelló |
| author_sort | Iñigo Moreno-Ocio |
| collection | DOAJ |
| description | A toxicogenomic approach was used for toxicity evaluation of arsenic in the aquatic environment, and differential gene expression was investigated from 24 h and 96 h water-only acute toxicity tests with the aquatic oligochaete, <i>Tubifex tubifex</i> (<i>Annelida</i>, <i>Clitellata</i>). Several toxicological endpoints (survival and autotomy) of the oligochaete and tissue residues were measured, and dose-response modelling of gene expression data was studied. A reference transcriptome of the aquatic oligochaete, <i>T. tubifex</i>, was reconstructed for the first time, and genes related to cell stress response (<i>Hsc70</i>, <i>Hsp10</i>, <i>Hsp60</i>, and <i>Hsp83</i>), energy metabolism (<i>COX1</i>), oxidative stress (<i>Cat</i>, <i>GSR</i>, and <i>MnSOD</i>), and the genes involved in the homeostasis of organisms (<i>CaM</i>, <i>RpS13</i>, and <i>UBE2</i>) were identified and characterised. The potential use of the genes identified for risk assessment in freshwater ecosystems as early biomarkers of arsenic toxicity is discussed. |
| first_indexed | 2024-04-24T18:11:58Z |
| format | Article |
| id | doaj.art-ffec7c610a6a439ba307108489fdcfe9 |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-04-24T18:11:58Z |
| publishDate | 2024-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-ffec7c610a6a439ba307108489fdcfe92024-03-27T13:45:54ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672024-03-01256338210.3390/ijms25063382Toxicogenomics of the Freshwater Oligochaete, <i>Tubifex tubifex</i> (<i>Annelida</i>, <i>Clitellata</i>), in Acute Water-Only Exposure to ArsenicIñigo Moreno-Ocio0Mónica Aquilino1Lola Llorente2Maite Martínez-Madrid3Pilar Rodríguez4Leire Méndez-Fernández5Rosario Planelló6Department of Zoology and Animal Cell Biology, University of the Basque Country (UPV/EHU), 48940 Leioa, SpainMolecular Entomology, Biomarkers and Environmental Stress Group, Faculty of Science, Universidad Nacional de Educación a Distancia (UNED), 28232 Las Rozas de Madrid, SpainMolecular Entomology, Biomarkers and Environmental Stress Group, Faculty of Science, Universidad Nacional de Educación a Distancia (UNED), 28232 Las Rozas de Madrid, SpainDepartment of Genetics, Physical Anthropology and Animal Physiology, University of the Basque Country (UPV/EHU), 48940 Leioa, SpainDepartment of Zoology and Animal Cell Biology, University of the Basque Country (UPV/EHU), 48940 Leioa, SpainDepartment of Genetics, Physical Anthropology and Animal Physiology, University of the Basque Country (UPV/EHU), 48940 Leioa, SpainMolecular Entomology, Biomarkers and Environmental Stress Group, Faculty of Science, Universidad Nacional de Educación a Distancia (UNED), 28232 Las Rozas de Madrid, SpainA toxicogenomic approach was used for toxicity evaluation of arsenic in the aquatic environment, and differential gene expression was investigated from 24 h and 96 h water-only acute toxicity tests with the aquatic oligochaete, <i>Tubifex tubifex</i> (<i>Annelida</i>, <i>Clitellata</i>). Several toxicological endpoints (survival and autotomy) of the oligochaete and tissue residues were measured, and dose-response modelling of gene expression data was studied. A reference transcriptome of the aquatic oligochaete, <i>T. tubifex</i>, was reconstructed for the first time, and genes related to cell stress response (<i>Hsc70</i>, <i>Hsp10</i>, <i>Hsp60</i>, and <i>Hsp83</i>), energy metabolism (<i>COX1</i>), oxidative stress (<i>Cat</i>, <i>GSR</i>, and <i>MnSOD</i>), and the genes involved in the homeostasis of organisms (<i>CaM</i>, <i>RpS13</i>, and <i>UBE2</i>) were identified and characterised. The potential use of the genes identified for risk assessment in freshwater ecosystems as early biomarkers of arsenic toxicity is discussed.https://www.mdpi.com/1422-0067/25/6/3382arsenicoligochaetatoxicity assaytranscriptional biomarkercell stress responseoxidative stress |
| spellingShingle | Iñigo Moreno-Ocio Mónica Aquilino Lola Llorente Maite Martínez-Madrid Pilar Rodríguez Leire Méndez-Fernández Rosario Planelló Toxicogenomics of the Freshwater Oligochaete, <i>Tubifex tubifex</i> (<i>Annelida</i>, <i>Clitellata</i>), in Acute Water-Only Exposure to Arsenic International Journal of Molecular Sciences arsenic oligochaeta toxicity assay transcriptional biomarker cell stress response oxidative stress |
| title | Toxicogenomics of the Freshwater Oligochaete, <i>Tubifex tubifex</i> (<i>Annelida</i>, <i>Clitellata</i>), in Acute Water-Only Exposure to Arsenic |
| title_full | Toxicogenomics of the Freshwater Oligochaete, <i>Tubifex tubifex</i> (<i>Annelida</i>, <i>Clitellata</i>), in Acute Water-Only Exposure to Arsenic |
| title_fullStr | Toxicogenomics of the Freshwater Oligochaete, <i>Tubifex tubifex</i> (<i>Annelida</i>, <i>Clitellata</i>), in Acute Water-Only Exposure to Arsenic |
| title_full_unstemmed | Toxicogenomics of the Freshwater Oligochaete, <i>Tubifex tubifex</i> (<i>Annelida</i>, <i>Clitellata</i>), in Acute Water-Only Exposure to Arsenic |
| title_short | Toxicogenomics of the Freshwater Oligochaete, <i>Tubifex tubifex</i> (<i>Annelida</i>, <i>Clitellata</i>), in Acute Water-Only Exposure to Arsenic |
| title_sort | toxicogenomics of the freshwater oligochaete i tubifex tubifex i i annelida i i clitellata i in acute water only exposure to arsenic |
| topic | arsenic oligochaeta toxicity assay transcriptional biomarker cell stress response oxidative stress |
| url | https://www.mdpi.com/1422-0067/25/6/3382 |
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