Detecting Pre-Analytically Delayed Blood Samples for Laboratory Diagnostics Using Raman Spectroscopy
In this proof-of-principle study, we systematically studied the potential of Raman spectroscopy for detecting pre-analytical delays in blood serum samples. Spectra from 330 samples from a liver cirrhosis cohort were acquired over the course of eight days, stored one day at room temperature, and stor...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2023-04-01
|
| Series: | International Journal of Molecular Sciences |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1422-0067/24/9/7853 |
| _version_ | 1797602594269429760 |
|---|---|
| author | Pascal Hunold Markus Fischer Carsten Olthoff Peter W. Hildebrand Thorsten Kaiser René Staritzbichler |
| author_facet | Pascal Hunold Markus Fischer Carsten Olthoff Peter W. Hildebrand Thorsten Kaiser René Staritzbichler |
| author_sort | Pascal Hunold |
| collection | DOAJ |
| description | In this proof-of-principle study, we systematically studied the potential of Raman spectroscopy for detecting pre-analytical delays in blood serum samples. Spectra from 330 samples from a liver cirrhosis cohort were acquired over the course of eight days, stored one day at room temperature, and stored subsequently at 4 °C. The spectra were then used to train Convolutional Neural Networks (CNN) to predict the delay to sample examination. We achieved 90% accuracy for binary classification of the serum samples in the groups “without delay” versus “delayed”. Spectra recorded on the first day could be distinguished clearly from all subsequent measurements. Distinguishing between spectra taken in the range from the second to the last day seems to be possible as well, but currently, with an accuracy of approximately 70% only. Importantly, filtering out the fluorescent background significantly reduces the precision of detection. |
| first_indexed | 2024-03-11T04:17:43Z |
| format | Article |
| id | doaj.art-ffed899d43b5419da0eec485060264d6 |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-11T04:17:43Z |
| publishDate | 2023-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-ffed899d43b5419da0eec485060264d62023-11-17T23:01:30ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672023-04-01249785310.3390/ijms24097853Detecting Pre-Analytically Delayed Blood Samples for Laboratory Diagnostics Using Raman SpectroscopyPascal Hunold0Markus Fischer1Carsten Olthoff2Peter W. Hildebrand3Thorsten Kaiser4René Staritzbichler5Institute of Laboratory Medicine, Clinical Chemistry and Molecular Diagnostics, University Hospital Leipzig, 04103 Leipzig, GermanyInstitute for Medical Physics and Biophysics, Leipzig University, 04107 Leipzig, GermanyInstitute of Laboratory Medicine, Clinical Chemistry and Molecular Diagnostics, University Hospital Leipzig, 04103 Leipzig, GermanyInstitute for Medical Physics and Biophysics, Leipzig University, 04107 Leipzig, GermanyInstitute of Laboratory Medicine, Clinical Chemistry and Molecular Diagnostics, University Hospital Leipzig, 04103 Leipzig, GermanyInstitute for Medical Physics and Biophysics, Leipzig University, 04107 Leipzig, GermanyIn this proof-of-principle study, we systematically studied the potential of Raman spectroscopy for detecting pre-analytical delays in blood serum samples. Spectra from 330 samples from a liver cirrhosis cohort were acquired over the course of eight days, stored one day at room temperature, and stored subsequently at 4 °C. The spectra were then used to train Convolutional Neural Networks (CNN) to predict the delay to sample examination. We achieved 90% accuracy for binary classification of the serum samples in the groups “without delay” versus “delayed”. Spectra recorded on the first day could be distinguished clearly from all subsequent measurements. Distinguishing between spectra taken in the range from the second to the last day seems to be possible as well, but currently, with an accuracy of approximately 70% only. Importantly, filtering out the fluorescent background significantly reduces the precision of detection.https://www.mdpi.com/1422-0067/24/9/7853laboratory medicine diagnosticspreclinical delayssample agequality assuranceRaman spectroscopy |
| spellingShingle | Pascal Hunold Markus Fischer Carsten Olthoff Peter W. Hildebrand Thorsten Kaiser René Staritzbichler Detecting Pre-Analytically Delayed Blood Samples for Laboratory Diagnostics Using Raman Spectroscopy International Journal of Molecular Sciences laboratory medicine diagnostics preclinical delays sample age quality assurance Raman spectroscopy |
| title | Detecting Pre-Analytically Delayed Blood Samples for Laboratory Diagnostics Using Raman Spectroscopy |
| title_full | Detecting Pre-Analytically Delayed Blood Samples for Laboratory Diagnostics Using Raman Spectroscopy |
| title_fullStr | Detecting Pre-Analytically Delayed Blood Samples for Laboratory Diagnostics Using Raman Spectroscopy |
| title_full_unstemmed | Detecting Pre-Analytically Delayed Blood Samples for Laboratory Diagnostics Using Raman Spectroscopy |
| title_short | Detecting Pre-Analytically Delayed Blood Samples for Laboratory Diagnostics Using Raman Spectroscopy |
| title_sort | detecting pre analytically delayed blood samples for laboratory diagnostics using raman spectroscopy |
| topic | laboratory medicine diagnostics preclinical delays sample age quality assurance Raman spectroscopy |
| url | https://www.mdpi.com/1422-0067/24/9/7853 |
| work_keys_str_mv | AT pascalhunold detectingpreanalyticallydelayedbloodsamplesforlaboratorydiagnosticsusingramanspectroscopy AT markusfischer detectingpreanalyticallydelayedbloodsamplesforlaboratorydiagnosticsusingramanspectroscopy AT carstenolthoff detectingpreanalyticallydelayedbloodsamplesforlaboratorydiagnosticsusingramanspectroscopy AT peterwhildebrand detectingpreanalyticallydelayedbloodsamplesforlaboratorydiagnosticsusingramanspectroscopy AT thorstenkaiser detectingpreanalyticallydelayedbloodsamplesforlaboratorydiagnosticsusingramanspectroscopy AT renestaritzbichler detectingpreanalyticallydelayedbloodsamplesforlaboratorydiagnosticsusingramanspectroscopy |