Transcriptional Responses of Dictyostelium discoideum Exposed to Different Classes of Bacteria
Dictyostelium discoideum amoebae feed by ingesting bacteria, then killing them in phagosomes. Ingestion and killing of different bacteria have been shown to rely on largely different molecular mechanisms. One would thus expect that D. discoideum adapts its ingestion and killing machinery when encoun...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2020-03-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/article/10.3389/fmicb.2020.00410/full |
| _version_ | 1818284886777135104 |
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| author | Otmane Lamrabet Astrid Melotti Frédéric Burdet Nabil Hanna Jackie Perrin Jahn Nitschke Marco Pagni Hubert Hilbi Thierry Soldati Pierre Cosson |
| author_facet | Otmane Lamrabet Astrid Melotti Frédéric Burdet Nabil Hanna Jackie Perrin Jahn Nitschke Marco Pagni Hubert Hilbi Thierry Soldati Pierre Cosson |
| author_sort | Otmane Lamrabet |
| collection | DOAJ |
| description | Dictyostelium discoideum amoebae feed by ingesting bacteria, then killing them in phagosomes. Ingestion and killing of different bacteria have been shown to rely on largely different molecular mechanisms. One would thus expect that D. discoideum adapts its ingestion and killing machinery when encountering different bacteria. In this study, we investigated by RNA sequencing if and how D. discoideum amoebae respond to the presence of different bacteria by modifying their gene expression patterns. Each bacterial species analyzed induced a specific modification of the transcriptome. Bacteria such as Bacillus subtilis, Klebsiella pneumoniae, or Mycobacterium marinum induced a specific and different transcriptional response, while Micrococcus luteus did not trigger a significant gene regulation. Although folate has been proposed to be one of the key molecules secreted by bacteria and recognized by hunting amoebae, it elicited a very specific and restricted transcriptional signature, distinct from that triggered by any bacteria analyzed here. Our results indicate that D. discoideum amoebae respond in a highly specific, almost non-overlapping manner to different species of bacteria. We additionally identify specific sets of genes that can be used as reporters of the response of D. discoideum to different bacteria. |
| first_indexed | 2024-12-13T00:59:56Z |
| format | Article |
| id | doaj.art-fff77f7db321436a87f6ff4d8e1735f8 |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-12-13T00:59:56Z |
| publishDate | 2020-03-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-fff77f7db321436a87f6ff4d8e1735f82022-12-22T00:04:43ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2020-03-011110.3389/fmicb.2020.00410517710Transcriptional Responses of Dictyostelium discoideum Exposed to Different Classes of BacteriaOtmane Lamrabet0Astrid Melotti1Frédéric Burdet2Nabil Hanna3Jackie Perrin4Jahn Nitschke5Marco Pagni6Hubert Hilbi7Thierry Soldati8Pierre Cosson9Department of Cell Physiology and Metabolism, Faculty of Medicine, University of Geneva, Geneva, SwitzerlandDepartment of Cell Physiology and Metabolism, Faculty of Medicine, University of Geneva, Geneva, SwitzerlandVital-IT Group, SIB, Swiss Institute of Bioinformatics, Lausanne, SwitzerlandDepartment of Biochemistry, Faculty of Science, University of Geneva, Geneva, SwitzerlandDepartment of Cell Physiology and Metabolism, Faculty of Medicine, University of Geneva, Geneva, SwitzerlandDepartment of Biochemistry, Faculty of Science, University of Geneva, Geneva, SwitzerlandVital-IT Group, SIB, Swiss Institute of Bioinformatics, Lausanne, SwitzerlandFaculty of Medicine, Institute of Medical Microbiology, University of Zurich, Zurich, SwitzerlandDepartment of Biochemistry, Faculty of Science, University of Geneva, Geneva, SwitzerlandDepartment of Cell Physiology and Metabolism, Faculty of Medicine, University of Geneva, Geneva, SwitzerlandDictyostelium discoideum amoebae feed by ingesting bacteria, then killing them in phagosomes. Ingestion and killing of different bacteria have been shown to rely on largely different molecular mechanisms. One would thus expect that D. discoideum adapts its ingestion and killing machinery when encountering different bacteria. In this study, we investigated by RNA sequencing if and how D. discoideum amoebae respond to the presence of different bacteria by modifying their gene expression patterns. Each bacterial species analyzed induced a specific modification of the transcriptome. Bacteria such as Bacillus subtilis, Klebsiella pneumoniae, or Mycobacterium marinum induced a specific and different transcriptional response, while Micrococcus luteus did not trigger a significant gene regulation. Although folate has been proposed to be one of the key molecules secreted by bacteria and recognized by hunting amoebae, it elicited a very specific and restricted transcriptional signature, distinct from that triggered by any bacteria analyzed here. Our results indicate that D. discoideum amoebae respond in a highly specific, almost non-overlapping manner to different species of bacteria. We additionally identify specific sets of genes that can be used as reporters of the response of D. discoideum to different bacteria.https://www.frontiersin.org/article/10.3389/fmicb.2020.00410/fullDictyostelium discoideumhost-pathogen interactionsRNA-seqKlebsiella pneumoniaeBacillus subtilisMycobacterium marinum |
| spellingShingle | Otmane Lamrabet Astrid Melotti Frédéric Burdet Nabil Hanna Jackie Perrin Jahn Nitschke Marco Pagni Hubert Hilbi Thierry Soldati Pierre Cosson Transcriptional Responses of Dictyostelium discoideum Exposed to Different Classes of Bacteria Frontiers in Microbiology Dictyostelium discoideum host-pathogen interactions RNA-seq Klebsiella pneumoniae Bacillus subtilis Mycobacterium marinum |
| title | Transcriptional Responses of Dictyostelium discoideum Exposed to Different Classes of Bacteria |
| title_full | Transcriptional Responses of Dictyostelium discoideum Exposed to Different Classes of Bacteria |
| title_fullStr | Transcriptional Responses of Dictyostelium discoideum Exposed to Different Classes of Bacteria |
| title_full_unstemmed | Transcriptional Responses of Dictyostelium discoideum Exposed to Different Classes of Bacteria |
| title_short | Transcriptional Responses of Dictyostelium discoideum Exposed to Different Classes of Bacteria |
| title_sort | transcriptional responses of dictyostelium discoideum exposed to different classes of bacteria |
| topic | Dictyostelium discoideum host-pathogen interactions RNA-seq Klebsiella pneumoniae Bacillus subtilis Mycobacterium marinum |
| url | https://www.frontiersin.org/article/10.3389/fmicb.2020.00410/full |
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