Strong isolation by distance among local populations of an endangered butterfly species (Euphydryas aurinia)
Abstract The marsh fritillary (Euphydryas aurinia) is a critically endangered butterfly species in Denmark known to be particularly vulnerable to habitat fragmentation due to its poor dispersal capacity. We identified and genotyped 318 novel SNP loci across 273 individuals obtained from 10 small and...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2021-09-01
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| Series: | Ecology and Evolution |
| Subjects: | |
| Online Access: | https://doi.org/10.1002/ece3.8027 |
| _version_ | 1819101502234427392 |
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| author | Cino Pertoldi Aritz Ruiz‐Gonzalez Simon Bahrndorff Nanna Renee Lauridsen Thøger Nisbeth Henriksen Anne Eskildsen Toke Thomas Høye |
| author_facet | Cino Pertoldi Aritz Ruiz‐Gonzalez Simon Bahrndorff Nanna Renee Lauridsen Thøger Nisbeth Henriksen Anne Eskildsen Toke Thomas Høye |
| author_sort | Cino Pertoldi |
| collection | DOAJ |
| description | Abstract The marsh fritillary (Euphydryas aurinia) is a critically endangered butterfly species in Denmark known to be particularly vulnerable to habitat fragmentation due to its poor dispersal capacity. We identified and genotyped 318 novel SNP loci across 273 individuals obtained from 10 small and fragmented populations in Denmark using a genotyping‐by‐sequencing (GBS) approach to investigate its population genetic structure. Our results showed clear genetic substructuring and highly significant population differentiation based on genetic divergence (FST) among the 10 populations. The populations clustered in three overall clusters, and due to further substructuring among these, it was possible to clearly distinguish six clusters in total. We found highly significant deviations from Hardy–Weinberg equilibrium due to heterozygote deficiency within every population investigated, which indicates substructuring and/or inbreeding (due to mating among closely related individuals). The stringent filtering procedure that we have applied to our genotype quality could have overestimated the heterozygote deficiency and the degree of substructuring of our clusters but is allowing relative comparisons of the genetic parameters among clusters. Genetic divergence increased significantly with geographic distance, suggesting limited gene flow at spatial scales comparable to the dispersal distance of individual butterflies and strong isolation by distance. Altogether, our results clearly indicate that the marsh fritillary populations are genetically isolated. Further, our results highlight that the relevant spatial scale for conservation of rare, low mobile species may be smaller than previously anticipated. |
| first_indexed | 2024-12-22T01:19:41Z |
| format | Article |
| id | doaj.art-fff86f3381f74e989a791df2e1dab449 |
| institution | Directory Open Access Journal |
| issn | 2045-7758 |
| language | English |
| last_indexed | 2024-12-22T01:19:41Z |
| publishDate | 2021-09-01 |
| publisher | Wiley |
| record_format | Article |
| series | Ecology and Evolution |
| spelling | doaj.art-fff86f3381f74e989a791df2e1dab4492022-12-21T18:43:46ZengWileyEcology and Evolution2045-77582021-09-011118127901280010.1002/ece3.8027Strong isolation by distance among local populations of an endangered butterfly species (Euphydryas aurinia)Cino Pertoldi0Aritz Ruiz‐Gonzalez1Simon Bahrndorff2Nanna Renee Lauridsen3Thøger Nisbeth Henriksen4Anne Eskildsen5Toke Thomas Høye6Department of Chemistry and Bioscience Aalborg University Aalborg DenmarkDepartment of Zoology and Animal Cell Biology University of the Basque Country UPV/EHU Vitoria‐Gasteiz SpainDepartment of Chemistry and Bioscience Aalborg University Aalborg DenmarkDepartment of Bioscience and Arctic Research Centre Aarhus University Rønde DenmarkDepartment of Bioscience and Arctic Research Centre Aarhus University Rønde DenmarkDepartment of Bioscience and Arctic Research Centre Aarhus University Rønde DenmarkDepartment of Bioscience and Arctic Research Centre Aarhus University Rønde DenmarkAbstract The marsh fritillary (Euphydryas aurinia) is a critically endangered butterfly species in Denmark known to be particularly vulnerable to habitat fragmentation due to its poor dispersal capacity. We identified and genotyped 318 novel SNP loci across 273 individuals obtained from 10 small and fragmented populations in Denmark using a genotyping‐by‐sequencing (GBS) approach to investigate its population genetic structure. Our results showed clear genetic substructuring and highly significant population differentiation based on genetic divergence (FST) among the 10 populations. The populations clustered in three overall clusters, and due to further substructuring among these, it was possible to clearly distinguish six clusters in total. We found highly significant deviations from Hardy–Weinberg equilibrium due to heterozygote deficiency within every population investigated, which indicates substructuring and/or inbreeding (due to mating among closely related individuals). The stringent filtering procedure that we have applied to our genotype quality could have overestimated the heterozygote deficiency and the degree of substructuring of our clusters but is allowing relative comparisons of the genetic parameters among clusters. Genetic divergence increased significantly with geographic distance, suggesting limited gene flow at spatial scales comparable to the dispersal distance of individual butterflies and strong isolation by distance. Altogether, our results clearly indicate that the marsh fritillary populations are genetically isolated. Further, our results highlight that the relevant spatial scale for conservation of rare, low mobile species may be smaller than previously anticipated.https://doi.org/10.1002/ece3.8027fragmentationgenetic driftgenotyping‐by‐sequencinginbreedingSNP |
| spellingShingle | Cino Pertoldi Aritz Ruiz‐Gonzalez Simon Bahrndorff Nanna Renee Lauridsen Thøger Nisbeth Henriksen Anne Eskildsen Toke Thomas Høye Strong isolation by distance among local populations of an endangered butterfly species (Euphydryas aurinia) Ecology and Evolution fragmentation genetic drift genotyping‐by‐sequencing inbreeding SNP |
| title | Strong isolation by distance among local populations of an endangered butterfly species (Euphydryas aurinia) |
| title_full | Strong isolation by distance among local populations of an endangered butterfly species (Euphydryas aurinia) |
| title_fullStr | Strong isolation by distance among local populations of an endangered butterfly species (Euphydryas aurinia) |
| title_full_unstemmed | Strong isolation by distance among local populations of an endangered butterfly species (Euphydryas aurinia) |
| title_short | Strong isolation by distance among local populations of an endangered butterfly species (Euphydryas aurinia) |
| title_sort | strong isolation by distance among local populations of an endangered butterfly species euphydryas aurinia |
| topic | fragmentation genetic drift genotyping‐by‐sequencing inbreeding SNP |
| url | https://doi.org/10.1002/ece3.8027 |
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