A Multi-Bioassay Integrated Approach to Assess the Antifouling Potential of the Cyanobacterial Metabolites Portoamides
The cyclic peptides portoamides produced by the cyanobacterium <i>Phormidium</i> sp. LEGE 05292 were previously isolated and their ability to condition microcommunities by allelopathic effect was described. These interesting bioactive properties are, however, still underexplored as their...
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2019-02-01
|
| Series: | Marine Drugs |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1660-3397/17/2/111 |
| _version_ | 1818001896199159808 |
|---|---|
| author | Jorge Antunes Sandra Pereira Tiago Ribeiro Jeffrey E. Plowman Ancy Thomas Stefan Clerens Alexandre Campos Vitor Vasconcelos Joana R. Almeida |
| author_facet | Jorge Antunes Sandra Pereira Tiago Ribeiro Jeffrey E. Plowman Ancy Thomas Stefan Clerens Alexandre Campos Vitor Vasconcelos Joana R. Almeida |
| author_sort | Jorge Antunes |
| collection | DOAJ |
| description | The cyclic peptides portoamides produced by the cyanobacterium <i>Phormidium</i> sp. LEGE 05292 were previously isolated and their ability to condition microcommunities by allelopathic effect was described. These interesting bioactive properties are, however, still underexplored as their biotechnological applications may be vast. This study aims to investigate the antifouling potential of portoamides, given that a challenge in the search for new environmentally friendly antifouling products is to find non-toxic natural alternatives with the ability to prevent colonization of different biofouling species, from bacteria to macroinvertebrates. A multi-bioassay approach was applied to assess portoamides antifouling properties, marine ecotoxicity and molecular mode of action. Results showed high effectiveness in the prevention of mussel larvae settlement (EC<sub>50</sub> = 3.16 µM), and also bioactivity towards growth and biofilm disruption of marine biofouling bacterial strains, while not showing toxicity towards both target and non-target species. Antifouling molecular targets in mussel larvae include energy metabolism modifications (failure in proton-transporting ATPases activity), structural alterations of the gills and protein and gene regulatory mechanisms. Overall, portoamides reveal a broad-spectrum bioactivity towards diverse biofouling species, including a non-toxic and reversible effect towards mussel larvae, showing potential to be incorporated as an active ingredient in antifouling coatings. |
| first_indexed | 2024-04-14T03:40:06Z |
| format | Article |
| id | doaj.art-fffa6c7ad1834d19a15aa07cf60704e1 |
| institution | Directory Open Access Journal |
| issn | 1660-3397 |
| language | English |
| last_indexed | 2024-04-14T03:40:06Z |
| publishDate | 2019-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Marine Drugs |
| spelling | doaj.art-fffa6c7ad1834d19a15aa07cf60704e12022-12-22T02:14:35ZengMDPI AGMarine Drugs1660-33972019-02-0117211110.3390/md17020111md17020111A Multi-Bioassay Integrated Approach to Assess the Antifouling Potential of the Cyanobacterial Metabolites PortoamidesJorge Antunes0Sandra Pereira1Tiago Ribeiro2Jeffrey E. Plowman3Ancy Thomas4Stefan Clerens5Alexandre Campos6Vitor Vasconcelos7Joana R. Almeida8Centro Interdisciplinar de Investigação Marinha e Ambiental (CIIMAR), Universidade do Porto, Terminal de Cruzeiros do Porto de Leixões, Av. General Norton de Matos s/n, 4450-208 Matosinhos, PortugalCentro Interdisciplinar de Investigação Marinha e Ambiental (CIIMAR), Universidade do Porto, Terminal de Cruzeiros do Porto de Leixões, Av. General Norton de Matos s/n, 4450-208 Matosinhos, PortugalCentro Interdisciplinar de Investigação Marinha e Ambiental (CIIMAR), Universidade do Porto, Terminal de Cruzeiros do Porto de Leixões, Av. General Norton de Matos s/n, 4450-208 Matosinhos, PortugalAgResearch Ltd., 1365 Springs Rd, Lincoln 7674, New ZealandAgResearch Ltd., 1365 Springs Rd, Lincoln 7674, New ZealandAgResearch Ltd., 1365 Springs Rd, Lincoln 7674, New ZealandCentro Interdisciplinar de Investigação Marinha e Ambiental (CIIMAR), Universidade do Porto, Terminal de Cruzeiros do Porto de Leixões, Av. General Norton de Matos s/n, 4450-208 Matosinhos, PortugalCentro Interdisciplinar de Investigação Marinha e Ambiental (CIIMAR), Universidade do Porto, Terminal de Cruzeiros do Porto de Leixões, Av. General Norton de Matos s/n, 4450-208 Matosinhos, PortugalCentro Interdisciplinar de Investigação Marinha e Ambiental (CIIMAR), Universidade do Porto, Terminal de Cruzeiros do Porto de Leixões, Av. General Norton de Matos s/n, 4450-208 Matosinhos, PortugalThe cyclic peptides portoamides produced by the cyanobacterium <i>Phormidium</i> sp. LEGE 05292 were previously isolated and their ability to condition microcommunities by allelopathic effect was described. These interesting bioactive properties are, however, still underexplored as their biotechnological applications may be vast. This study aims to investigate the antifouling potential of portoamides, given that a challenge in the search for new environmentally friendly antifouling products is to find non-toxic natural alternatives with the ability to prevent colonization of different biofouling species, from bacteria to macroinvertebrates. A multi-bioassay approach was applied to assess portoamides antifouling properties, marine ecotoxicity and molecular mode of action. Results showed high effectiveness in the prevention of mussel larvae settlement (EC<sub>50</sub> = 3.16 µM), and also bioactivity towards growth and biofilm disruption of marine biofouling bacterial strains, while not showing toxicity towards both target and non-target species. Antifouling molecular targets in mussel larvae include energy metabolism modifications (failure in proton-transporting ATPases activity), structural alterations of the gills and protein and gene regulatory mechanisms. Overall, portoamides reveal a broad-spectrum bioactivity towards diverse biofouling species, including a non-toxic and reversible effect towards mussel larvae, showing potential to be incorporated as an active ingredient in antifouling coatings.https://www.mdpi.com/1660-3397/17/2/111biofoulingantifoulingcyanobacterianatural productsmarine biofilmsmode of action |
| spellingShingle | Jorge Antunes Sandra Pereira Tiago Ribeiro Jeffrey E. Plowman Ancy Thomas Stefan Clerens Alexandre Campos Vitor Vasconcelos Joana R. Almeida A Multi-Bioassay Integrated Approach to Assess the Antifouling Potential of the Cyanobacterial Metabolites Portoamides Marine Drugs biofouling antifouling cyanobacteria natural products marine biofilms mode of action |
| title | A Multi-Bioassay Integrated Approach to Assess the Antifouling Potential of the Cyanobacterial Metabolites Portoamides |
| title_full | A Multi-Bioassay Integrated Approach to Assess the Antifouling Potential of the Cyanobacterial Metabolites Portoamides |
| title_fullStr | A Multi-Bioassay Integrated Approach to Assess the Antifouling Potential of the Cyanobacterial Metabolites Portoamides |
| title_full_unstemmed | A Multi-Bioassay Integrated Approach to Assess the Antifouling Potential of the Cyanobacterial Metabolites Portoamides |
| title_short | A Multi-Bioassay Integrated Approach to Assess the Antifouling Potential of the Cyanobacterial Metabolites Portoamides |
| title_sort | multi bioassay integrated approach to assess the antifouling potential of the cyanobacterial metabolites portoamides |
| topic | biofouling antifouling cyanobacteria natural products marine biofilms mode of action |
| url | https://www.mdpi.com/1660-3397/17/2/111 |
| work_keys_str_mv | AT jorgeantunes amultibioassayintegratedapproachtoassesstheantifoulingpotentialofthecyanobacterialmetabolitesportoamides AT sandrapereira amultibioassayintegratedapproachtoassesstheantifoulingpotentialofthecyanobacterialmetabolitesportoamides AT tiagoribeiro amultibioassayintegratedapproachtoassesstheantifoulingpotentialofthecyanobacterialmetabolitesportoamides AT jeffreyeplowman amultibioassayintegratedapproachtoassesstheantifoulingpotentialofthecyanobacterialmetabolitesportoamides AT ancythomas amultibioassayintegratedapproachtoassesstheantifoulingpotentialofthecyanobacterialmetabolitesportoamides AT stefanclerens amultibioassayintegratedapproachtoassesstheantifoulingpotentialofthecyanobacterialmetabolitesportoamides AT alexandrecampos amultibioassayintegratedapproachtoassesstheantifoulingpotentialofthecyanobacterialmetabolitesportoamides AT vitorvasconcelos amultibioassayintegratedapproachtoassesstheantifoulingpotentialofthecyanobacterialmetabolitesportoamides AT joanaralmeida amultibioassayintegratedapproachtoassesstheantifoulingpotentialofthecyanobacterialmetabolitesportoamides AT jorgeantunes multibioassayintegratedapproachtoassesstheantifoulingpotentialofthecyanobacterialmetabolitesportoamides AT sandrapereira multibioassayintegratedapproachtoassesstheantifoulingpotentialofthecyanobacterialmetabolitesportoamides AT tiagoribeiro multibioassayintegratedapproachtoassesstheantifoulingpotentialofthecyanobacterialmetabolitesportoamides AT jeffreyeplowman multibioassayintegratedapproachtoassesstheantifoulingpotentialofthecyanobacterialmetabolitesportoamides AT ancythomas multibioassayintegratedapproachtoassesstheantifoulingpotentialofthecyanobacterialmetabolitesportoamides AT stefanclerens multibioassayintegratedapproachtoassesstheantifoulingpotentialofthecyanobacterialmetabolitesportoamides AT alexandrecampos multibioassayintegratedapproachtoassesstheantifoulingpotentialofthecyanobacterialmetabolitesportoamides AT vitorvasconcelos multibioassayintegratedapproachtoassesstheantifoulingpotentialofthecyanobacterialmetabolitesportoamides AT joanaralmeida multibioassayintegratedapproachtoassesstheantifoulingpotentialofthecyanobacterialmetabolitesportoamides |