DexA70, the Truncated Form of a Self-Produced Dextranase, Effectively Disrupts Streptococcus mutans Biofilm
Billions of people suffer from dental caries every year in spite of the effort to reduce the prevalence over the past few decades. Streptococcus mutans is the leading member of a specific group of cariogenic bacteria that cause dental caries. S. mutans forms biofilm, which is highly resistant to har...
| Main Authors: | , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2021-09-01
|
| Series: | Frontiers in Microbiology |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2021.737458/full |
| _version_ | 1819147805576396800 |
|---|---|
| author | Nan Liu Xin Li Maofeng Wang Fengyu Zhang Chuandong Wang Kundi Zhang Hongwei Wang Sujuan Xu Wei Hu Lichuan Gu |
| author_facet | Nan Liu Xin Li Maofeng Wang Fengyu Zhang Chuandong Wang Kundi Zhang Hongwei Wang Sujuan Xu Wei Hu Lichuan Gu |
| author_sort | Nan Liu |
| collection | DOAJ |
| description | Billions of people suffer from dental caries every year in spite of the effort to reduce the prevalence over the past few decades. Streptococcus mutans is the leading member of a specific group of cariogenic bacteria that cause dental caries. S. mutans forms biofilm, which is highly resistant to harsh environment, host immunity, and antimicrobial treatments. In this study, we found that S. mutans biofilm is highly resistant to both antimicrobial agents and lysozyme. DexA70, the truncated form of DexA (amino acids 100–732), a dextranase in S. mutans, prevents S. mutans biofilm formation and disassembles existing biofilms within minutes at nanomolar concentrations when supplied exogenously. DexA70 treatment markedly enhances biofilm sensitivity to antimicrobial agents and lysozyme, indicating its great potential in combating biofilm-related dental caries. |
| first_indexed | 2024-12-22T13:35:39Z |
| format | Article |
| id | doaj.art-fffaf80b870841c4a2750c2399f15afe |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-12-22T13:35:39Z |
| publishDate | 2021-09-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-fffaf80b870841c4a2750c2399f15afe2022-12-21T18:24:04ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2021-09-011210.3389/fmicb.2021.737458737458DexA70, the Truncated Form of a Self-Produced Dextranase, Effectively Disrupts Streptococcus mutans BiofilmNan LiuXin LiMaofeng WangFengyu ZhangChuandong WangKundi ZhangHongwei WangSujuan XuWei HuLichuan GuBillions of people suffer from dental caries every year in spite of the effort to reduce the prevalence over the past few decades. Streptococcus mutans is the leading member of a specific group of cariogenic bacteria that cause dental caries. S. mutans forms biofilm, which is highly resistant to harsh environment, host immunity, and antimicrobial treatments. In this study, we found that S. mutans biofilm is highly resistant to both antimicrobial agents and lysozyme. DexA70, the truncated form of DexA (amino acids 100–732), a dextranase in S. mutans, prevents S. mutans biofilm formation and disassembles existing biofilms within minutes at nanomolar concentrations when supplied exogenously. DexA70 treatment markedly enhances biofilm sensitivity to antimicrobial agents and lysozyme, indicating its great potential in combating biofilm-related dental caries.https://www.frontiersin.org/articles/10.3389/fmicb.2021.737458/fulldental cariesStreptococcus mutansbiofilmpolysaccharidesDexA70lysozyme |
| spellingShingle | Nan Liu Xin Li Maofeng Wang Fengyu Zhang Chuandong Wang Kundi Zhang Hongwei Wang Sujuan Xu Wei Hu Lichuan Gu DexA70, the Truncated Form of a Self-Produced Dextranase, Effectively Disrupts Streptococcus mutans Biofilm Frontiers in Microbiology dental caries Streptococcus mutans biofilm polysaccharides DexA70 lysozyme |
| title | DexA70, the Truncated Form of a Self-Produced Dextranase, Effectively Disrupts Streptococcus mutans Biofilm |
| title_full | DexA70, the Truncated Form of a Self-Produced Dextranase, Effectively Disrupts Streptococcus mutans Biofilm |
| title_fullStr | DexA70, the Truncated Form of a Self-Produced Dextranase, Effectively Disrupts Streptococcus mutans Biofilm |
| title_full_unstemmed | DexA70, the Truncated Form of a Self-Produced Dextranase, Effectively Disrupts Streptococcus mutans Biofilm |
| title_short | DexA70, the Truncated Form of a Self-Produced Dextranase, Effectively Disrupts Streptococcus mutans Biofilm |
| title_sort | dexa70 the truncated form of a self produced dextranase effectively disrupts streptococcus mutans biofilm |
| topic | dental caries Streptococcus mutans biofilm polysaccharides DexA70 lysozyme |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2021.737458/full |
| work_keys_str_mv | AT nanliu dexa70thetruncatedformofaselfproduceddextranaseeffectivelydisruptsstreptococcusmutansbiofilm AT xinli dexa70thetruncatedformofaselfproduceddextranaseeffectivelydisruptsstreptococcusmutansbiofilm AT maofengwang dexa70thetruncatedformofaselfproduceddextranaseeffectivelydisruptsstreptococcusmutansbiofilm AT fengyuzhang dexa70thetruncatedformofaselfproduceddextranaseeffectivelydisruptsstreptococcusmutansbiofilm AT chuandongwang dexa70thetruncatedformofaselfproduceddextranaseeffectivelydisruptsstreptococcusmutansbiofilm AT kundizhang dexa70thetruncatedformofaselfproduceddextranaseeffectivelydisruptsstreptococcusmutansbiofilm AT hongweiwang dexa70thetruncatedformofaselfproduceddextranaseeffectivelydisruptsstreptococcusmutansbiofilm AT sujuanxu dexa70thetruncatedformofaselfproduceddextranaseeffectivelydisruptsstreptococcusmutansbiofilm AT weihu dexa70thetruncatedformofaselfproduceddextranaseeffectivelydisruptsstreptococcusmutansbiofilm AT lichuangu dexa70thetruncatedformofaselfproduceddextranaseeffectivelydisruptsstreptococcusmutansbiofilm |