Cadmium tolerance in Elodea canadensis Michx: Subcellular distribution and metabolomic analysis
The aquatic plant Elodea canadensis is considered a good candidate for ecotoxicological investigations. Cadmium (Cd) is a widespread contaminant in aquatic systems. In this study, to better elucidate the underlying tolerance mechanism and molecular impact of environmentally relevant Cd concentration...
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| Format: | Article |
| Language: | English |
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Elsevier
2023-05-01
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| Series: | Ecotoxicology and Environmental Safety |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S0147651323004098 |
| _version_ | 1797840516614717440 |
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| author | Qinsong Xu Wenjing Qiu Tinting Lin Yeyuping Yang Yuji Jiang |
| author_facet | Qinsong Xu Wenjing Qiu Tinting Lin Yeyuping Yang Yuji Jiang |
| author_sort | Qinsong Xu |
| collection | DOAJ |
| description | The aquatic plant Elodea canadensis is considered a good candidate for ecotoxicological investigations. Cadmium (Cd) is a widespread contaminant in aquatic systems. In this study, to better elucidate the underlying tolerance mechanism and molecular impact of environmentally relevant Cd concentration in aquatic plants, subcellular distribution, chemical forms, and gas chromatography–mass spectrometry-based non-targeted metabolomics profiles were comprehensively analyzed in E. canadensis subjected to 0 and 10 µM Cd treatment for 5 d. Subcellular fractionation analysis of Cd-containing leaves showed that 67% of Cd was compartmentalized in cell wall followed by the soluble fraction (24 %) and organelles (9 %). The majority of Cd (90 %) was found in the extraction using 1 M NaCl. Metabolomic analysis using unsupervised principal component analyses and a supervised partial least squares discriminant analysis revealed clear differences in metabolic profiles between the two groups, demonstrating the metabolic effects of Cd. The 155 identified compounds altered by Cd were mainly from primary metabolism, including sugars, amino acids, organic acids, and their derivatives. Secondary metabolites such as polyphenols and phenolamides were also detected. The massive up-regulation of metabolites, including trehalose, proline, sarcosine, nicotianamine, putrescine, α-ketoglutaric acid, citric acid, and phytol might represent a detoxification mechanism. These findings highlighted the mechanistic strategies that E. canadensis employs to defend against Cd toxicity. |
| first_indexed | 2024-04-09T16:16:37Z |
| format | Article |
| id | doaj.art-fffbd7b3cc694a19ba3636e67ef6735b |
| institution | Directory Open Access Journal |
| issn | 0147-6513 |
| language | English |
| last_indexed | 2024-04-09T16:16:37Z |
| publishDate | 2023-05-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Ecotoxicology and Environmental Safety |
| spelling | doaj.art-fffbd7b3cc694a19ba3636e67ef6735b2023-04-24T04:37:42ZengElsevierEcotoxicology and Environmental Safety0147-65132023-05-01256114905Cadmium tolerance in Elodea canadensis Michx: Subcellular distribution and metabolomic analysisQinsong Xu0Wenjing Qiu1Tinting Lin2Yeyuping Yang3Yuji Jiang4College of Life Science, Nanjing Normal University, Nanjing 210023, China; Corresponding author.College of Life Science, Nanjing Normal University, Nanjing 210023, ChinaCollege of Life Science, Nanjing Normal University, Nanjing 210023, ChinaCollege of Life Science, Nanjing Normal University, Nanjing 210023, ChinaState Key Laboratory of Soil and Sustainable Agriculture, Institute of Soil Science, Chinese Academy of Sciences, Nanjing 210008, China; Ecological Experimental Station of Red Soil, Chinese Academy of Sciences, Yingtan 335211, China; Corresponding author at: State Key Laboratory of Soil and Sustainable Agriculture, Institute of Soil Science, Chinese Academy of Sciences, Nanjing 210008, China.The aquatic plant Elodea canadensis is considered a good candidate for ecotoxicological investigations. Cadmium (Cd) is a widespread contaminant in aquatic systems. In this study, to better elucidate the underlying tolerance mechanism and molecular impact of environmentally relevant Cd concentration in aquatic plants, subcellular distribution, chemical forms, and gas chromatography–mass spectrometry-based non-targeted metabolomics profiles were comprehensively analyzed in E. canadensis subjected to 0 and 10 µM Cd treatment for 5 d. Subcellular fractionation analysis of Cd-containing leaves showed that 67% of Cd was compartmentalized in cell wall followed by the soluble fraction (24 %) and organelles (9 %). The majority of Cd (90 %) was found in the extraction using 1 M NaCl. Metabolomic analysis using unsupervised principal component analyses and a supervised partial least squares discriminant analysis revealed clear differences in metabolic profiles between the two groups, demonstrating the metabolic effects of Cd. The 155 identified compounds altered by Cd were mainly from primary metabolism, including sugars, amino acids, organic acids, and their derivatives. Secondary metabolites such as polyphenols and phenolamides were also detected. The massive up-regulation of metabolites, including trehalose, proline, sarcosine, nicotianamine, putrescine, α-ketoglutaric acid, citric acid, and phytol might represent a detoxification mechanism. These findings highlighted the mechanistic strategies that E. canadensis employs to defend against Cd toxicity.http://www.sciencedirect.com/science/article/pii/S0147651323004098Cd stressElodea canadensisSubcellular distributionChemical fractionMetabolomic profilingDefense |
| spellingShingle | Qinsong Xu Wenjing Qiu Tinting Lin Yeyuping Yang Yuji Jiang Cadmium tolerance in Elodea canadensis Michx: Subcellular distribution and metabolomic analysis Ecotoxicology and Environmental Safety Cd stress Elodea canadensis Subcellular distribution Chemical fraction Metabolomic profiling Defense |
| title | Cadmium tolerance in Elodea canadensis Michx: Subcellular distribution and metabolomic analysis |
| title_full | Cadmium tolerance in Elodea canadensis Michx: Subcellular distribution and metabolomic analysis |
| title_fullStr | Cadmium tolerance in Elodea canadensis Michx: Subcellular distribution and metabolomic analysis |
| title_full_unstemmed | Cadmium tolerance in Elodea canadensis Michx: Subcellular distribution and metabolomic analysis |
| title_short | Cadmium tolerance in Elodea canadensis Michx: Subcellular distribution and metabolomic analysis |
| title_sort | cadmium tolerance in elodea canadensis michx subcellular distribution and metabolomic analysis |
| topic | Cd stress Elodea canadensis Subcellular distribution Chemical fraction Metabolomic profiling Defense |
| url | http://www.sciencedirect.com/science/article/pii/S0147651323004098 |
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