Effect of the Combination of <i>Clostridium butyricum</i> and Mycelium of <i>Phellinus igniarius</i> on Intestinal Microbiota and Serum Metabolites in DSS-Induced Colitis
<i>Clostridium butyricum</i> (CB) and <i>Phellinus igniarius</i> (PI) have anti-inflammatory, immune regulation, anti-tumor, and other functions. This study aimed to explore the therapeutic effect of CB and mycelium of PI (MPI) alone and in combination on colitis mice induced...
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MDPI AG
2024-01-01
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| Series: | Nutrients |
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| author | Rou Zhao Qiaoyi Zhang Ting Huang Yun Tian Guiping Guan Yuanshan Lin |
| author_facet | Rou Zhao Qiaoyi Zhang Ting Huang Yun Tian Guiping Guan Yuanshan Lin |
| author_sort | Rou Zhao |
| collection | DOAJ |
| description | <i>Clostridium butyricum</i> (CB) and <i>Phellinus igniarius</i> (PI) have anti-inflammatory, immune regulation, anti-tumor, and other functions. This study aimed to explore the therapeutic effect of CB and mycelium of PI (MPI) alone and in combination on colitis mice induced by dextran sodium sulfate (DSS). Mice were randomly assigned to five groups: (1) control (CTRL), (2) DSS, (3) CB, (4) MPI, and (5) CB + MPI (CON). The weight of the mice was recorded daily during the experiment, and the length of the colon was measured on the last day of the experiment. The colons were collected for hematoxylin and eosin staining, colon contents were collected for intestinal flora analysis, and serum was collected for metabolite analysis. The results showed that compared with the DSS group, CB, MPI, and CON treatments inhibited the weight loss and colon length shortening caused by DSS, significantly increased the concentrations of interleukin (IL)-4, IL-10, and superoxide dismutase, and significantly decreased the concentrations of IL-6, tumor necrosis factor-α, and myeloperoxidase. Gene sequence analysis of 16S rRNA showed that CB, MPI, and CON treatments changed the composition and structure of intestinal microorganisms. Metabolome results showed that CB, MPI, and CON treatments changed serum metabolites in DSS-treated mice, including dodecenoylcarnitine, L-urobilinogen, and citric acid. In conclusion, CB, MPI, and CON treatments alleviated DSS-induced colitis in mice by regulating intestinal flora and metabolites, with the CON group having the best effect. |
| first_indexed | 2024-03-08T15:00:58Z |
| format | Article |
| id | doaj.art-fffd26d2402a42d6a2a81ce79f023e1b |
| institution | Directory Open Access Journal |
| issn | 2072-6643 |
| language | English |
| last_indexed | 2024-03-08T15:00:58Z |
| publishDate | 2024-01-01 |
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| spelling | doaj.art-fffd26d2402a42d6a2a81ce79f023e1b2024-01-10T15:05:44ZengMDPI AGNutrients2072-66432024-01-0116115310.3390/nu16010153Effect of the Combination of <i>Clostridium butyricum</i> and Mycelium of <i>Phellinus igniarius</i> on Intestinal Microbiota and Serum Metabolites in DSS-Induced ColitisRou Zhao0Qiaoyi Zhang1Ting Huang2Yun Tian3Guiping Guan4Yuanshan Lin5College of Bioscience and Biotechnology, Hunan Agricultural University, Changsha 410128, ChinaAgricultural Bioengineering Institute, Changsha 410128, ChinaAgricultural Bioengineering Institute, Changsha 410128, ChinaAgricultural Bioengineering Institute, Changsha 410128, ChinaAgricultural Bioengineering Institute, Changsha 410128, ChinaCollege of Bioscience and Biotechnology, Hunan Agricultural University, Changsha 410128, China<i>Clostridium butyricum</i> (CB) and <i>Phellinus igniarius</i> (PI) have anti-inflammatory, immune regulation, anti-tumor, and other functions. This study aimed to explore the therapeutic effect of CB and mycelium of PI (MPI) alone and in combination on colitis mice induced by dextran sodium sulfate (DSS). Mice were randomly assigned to five groups: (1) control (CTRL), (2) DSS, (3) CB, (4) MPI, and (5) CB + MPI (CON). The weight of the mice was recorded daily during the experiment, and the length of the colon was measured on the last day of the experiment. The colons were collected for hematoxylin and eosin staining, colon contents were collected for intestinal flora analysis, and serum was collected for metabolite analysis. The results showed that compared with the DSS group, CB, MPI, and CON treatments inhibited the weight loss and colon length shortening caused by DSS, significantly increased the concentrations of interleukin (IL)-4, IL-10, and superoxide dismutase, and significantly decreased the concentrations of IL-6, tumor necrosis factor-α, and myeloperoxidase. Gene sequence analysis of 16S rRNA showed that CB, MPI, and CON treatments changed the composition and structure of intestinal microorganisms. Metabolome results showed that CB, MPI, and CON treatments changed serum metabolites in DSS-treated mice, including dodecenoylcarnitine, L-urobilinogen, and citric acid. In conclusion, CB, MPI, and CON treatments alleviated DSS-induced colitis in mice by regulating intestinal flora and metabolites, with the CON group having the best effect.https://www.mdpi.com/2072-6643/16/1/153<i>Clostridium butyricum</i>mycelium of <i>Phellinus igniarius</i>colitisintestinal floraserum metabolome |
| spellingShingle | Rou Zhao Qiaoyi Zhang Ting Huang Yun Tian Guiping Guan Yuanshan Lin Effect of the Combination of <i>Clostridium butyricum</i> and Mycelium of <i>Phellinus igniarius</i> on Intestinal Microbiota and Serum Metabolites in DSS-Induced Colitis Nutrients <i>Clostridium butyricum</i> mycelium of <i>Phellinus igniarius</i> colitis intestinal flora serum metabolome |
| title | Effect of the Combination of <i>Clostridium butyricum</i> and Mycelium of <i>Phellinus igniarius</i> on Intestinal Microbiota and Serum Metabolites in DSS-Induced Colitis |
| title_full | Effect of the Combination of <i>Clostridium butyricum</i> and Mycelium of <i>Phellinus igniarius</i> on Intestinal Microbiota and Serum Metabolites in DSS-Induced Colitis |
| title_fullStr | Effect of the Combination of <i>Clostridium butyricum</i> and Mycelium of <i>Phellinus igniarius</i> on Intestinal Microbiota and Serum Metabolites in DSS-Induced Colitis |
| title_full_unstemmed | Effect of the Combination of <i>Clostridium butyricum</i> and Mycelium of <i>Phellinus igniarius</i> on Intestinal Microbiota and Serum Metabolites in DSS-Induced Colitis |
| title_short | Effect of the Combination of <i>Clostridium butyricum</i> and Mycelium of <i>Phellinus igniarius</i> on Intestinal Microbiota and Serum Metabolites in DSS-Induced Colitis |
| title_sort | effect of the combination of i clostridium butyricum i and mycelium of i phellinus igniarius i on intestinal microbiota and serum metabolites in dss induced colitis |
| topic | <i>Clostridium butyricum</i> mycelium of <i>Phellinus igniarius</i> colitis intestinal flora serum metabolome |
| url | https://www.mdpi.com/2072-6643/16/1/153 |
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