<i>Pseudomonas syringae</i> Infection Modifies Chlorophyll Fluorescence in <i>Nicotiana tabacum</i>
The system <i>Nicotiana tabacum</i> L.—<i>Pseudomonas syringae</i> VAN HALL pv. <i>tomato</i> (Pto) DC3000 was investigated at a low inoculation level (c. 5 × 10<sup>5</sup> colony-forming units (CFU) mL<sup>–1</sup>) such as it occurs in t...
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MDPI AG
2022-09-01
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| Series: | Agriculture |
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| author | Magdalena Tomaszewska-Sowa Norbert Keutgen Tomáš Lošák Anna Figas Anna J. Keutgen |
| author_facet | Magdalena Tomaszewska-Sowa Norbert Keutgen Tomáš Lošák Anna Figas Anna J. Keutgen |
| author_sort | Magdalena Tomaszewska-Sowa |
| collection | DOAJ |
| description | The system <i>Nicotiana tabacum</i> L.—<i>Pseudomonas syringae</i> VAN HALL pv. <i>tomato</i> (Pto) DC3000 was investigated at a low inoculation level (c. 5 × 10<sup>5</sup> colony-forming units (CFU) mL<sup>–1</sup>) such as it occurs in the field. The aim of this study was to test the hypothesis that <i>N. tabacum</i>, a non-host of Pto DC3000, improved the PSII efficiency in inoculated leaves compared with control detached leaves. Visible symptoms at the infected area were not detected within 14 days. Chlorophyll (Chl) <i>a</i> fluorescence was measured 6–7 days after inoculation of detached leaves. Compared with the control, the actual photochemical quantum yield of photosystem (PS) II was higher in the inoculated leaves at the expense of the fraction of heat dissipated by photo-inactivated non-functional centers. In addition, the fraction of open PSII reaction centers (RCs) was higher in inoculated leaves. Maximum fluorescence in the dark-adapted detached inoculated leaves, as a measure of the absorbed energy, was lower than in control leaves. The lower capacity to absorb energy in combination with a higher fraction of open PSII RCs is interpreted as an acclimation to limit over-excitation and to reduce heat dissipation. This should limit the production of reactive oxygen species and reduce the probability of a hypersensitive response (HR), which represents an expensive cell-death program for the plant. |
| first_indexed | 2024-03-10T01:01:10Z |
| format | Article |
| id | doaj.art-fffd6e569e104a7e8355604b9e368fe2 |
| institution | Directory Open Access Journal |
| issn | 2077-0472 |
| language | English |
| last_indexed | 2024-03-10T01:01:10Z |
| publishDate | 2022-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Agriculture |
| spelling | doaj.art-fffd6e569e104a7e8355604b9e368fe22023-11-23T14:35:06ZengMDPI AGAgriculture2077-04722022-09-01129150410.3390/agriculture12091504<i>Pseudomonas syringae</i> Infection Modifies Chlorophyll Fluorescence in <i>Nicotiana tabacum</i>Magdalena Tomaszewska-Sowa0Norbert Keutgen1Tomáš Lošák2Anna Figas3Anna J. Keutgen4Department of Agricultural Biotechnology, Bydgoszcz University of Science and Technology, Bernardyńska 6, 85-029 Bydgoszcz, PolandDepartment of Agricultural Biotechnology, Bydgoszcz University of Science and Technology, Bernardyńska 6, 85-029 Bydgoszcz, PolandDepartment of Environmentalistics and Natural Resources, Faculty of Regional Development and International Studies, Mendel University in Brno, Zemědělská 1, 613 00 Brno, Czech RepublicDepartment of Agricultural Biotechnology, Bydgoszcz University of Science and Technology, Bernardyńska 6, 85-029 Bydgoszcz, PolandDepartment of Crop Sciences, Institute of Vegetables and Ornamentals, University of Natural Resources and Life Sciences, Gregor Mendel Str. 33, 1180 Vienna, AustriaThe system <i>Nicotiana tabacum</i> L.—<i>Pseudomonas syringae</i> VAN HALL pv. <i>tomato</i> (Pto) DC3000 was investigated at a low inoculation level (c. 5 × 10<sup>5</sup> colony-forming units (CFU) mL<sup>–1</sup>) such as it occurs in the field. The aim of this study was to test the hypothesis that <i>N. tabacum</i>, a non-host of Pto DC3000, improved the PSII efficiency in inoculated leaves compared with control detached leaves. Visible symptoms at the infected area were not detected within 14 days. Chlorophyll (Chl) <i>a</i> fluorescence was measured 6–7 days after inoculation of detached leaves. Compared with the control, the actual photochemical quantum yield of photosystem (PS) II was higher in the inoculated leaves at the expense of the fraction of heat dissipated by photo-inactivated non-functional centers. In addition, the fraction of open PSII reaction centers (RCs) was higher in inoculated leaves. Maximum fluorescence in the dark-adapted detached inoculated leaves, as a measure of the absorbed energy, was lower than in control leaves. The lower capacity to absorb energy in combination with a higher fraction of open PSII RCs is interpreted as an acclimation to limit over-excitation and to reduce heat dissipation. This should limit the production of reactive oxygen species and reduce the probability of a hypersensitive response (HR), which represents an expensive cell-death program for the plant.https://www.mdpi.com/2077-0472/12/9/1504<i>Pseudomonas syringae</i> VAN HALL pv. <i>tomato</i> DC3000<i>Nicotiana tabacum</i> L.steady state fluorometryincompatible host plant—pathogen interactionleaf detachment |
| spellingShingle | Magdalena Tomaszewska-Sowa Norbert Keutgen Tomáš Lošák Anna Figas Anna J. Keutgen <i>Pseudomonas syringae</i> Infection Modifies Chlorophyll Fluorescence in <i>Nicotiana tabacum</i> Agriculture <i>Pseudomonas syringae</i> VAN HALL pv. <i>tomato</i> DC3000 <i>Nicotiana tabacum</i> L. steady state fluorometry incompatible host plant—pathogen interaction leaf detachment |
| title | <i>Pseudomonas syringae</i> Infection Modifies Chlorophyll Fluorescence in <i>Nicotiana tabacum</i> |
| title_full | <i>Pseudomonas syringae</i> Infection Modifies Chlorophyll Fluorescence in <i>Nicotiana tabacum</i> |
| title_fullStr | <i>Pseudomonas syringae</i> Infection Modifies Chlorophyll Fluorescence in <i>Nicotiana tabacum</i> |
| title_full_unstemmed | <i>Pseudomonas syringae</i> Infection Modifies Chlorophyll Fluorescence in <i>Nicotiana tabacum</i> |
| title_short | <i>Pseudomonas syringae</i> Infection Modifies Chlorophyll Fluorescence in <i>Nicotiana tabacum</i> |
| title_sort | i pseudomonas syringae i infection modifies chlorophyll fluorescence in i nicotiana tabacum i |
| topic | <i>Pseudomonas syringae</i> VAN HALL pv. <i>tomato</i> DC3000 <i>Nicotiana tabacum</i> L. steady state fluorometry incompatible host plant—pathogen interaction leaf detachment |
| url | https://www.mdpi.com/2077-0472/12/9/1504 |
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