Detecting Kinase Activities from Single Cell Lysate Using Concentration-Enhanced Mobility Shift Assay

Detecting Kinase Activities from Single Cell Lysate Using Concentration-Enhanced Mobility Shift Assay

Electrokinetic preconcentration coupled with mobility shift assays can give rise to very high detection sensitivities. We describe a microfluidic device that utilizes this principle to detect cellular kinase activities by simultaneously concentrating and separating substrate peptides with different...

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Bibliographic Details
Main Authors: Cheow, Lih Feng, Sarkar, Aniruddh, Kolitz, Sarah, Han, Jongyoon, Lauffenburger, Douglas A.
Other Authors: Massachusetts Institute of Technology. Department of Biological Engineering
Format: Article
Language:en_US
Published: American Chemical Society (ACS) 2015
Online Access:http://hdl.handle.net/1721.1/100245
https://orcid.org/0000-0001-7215-1439

Internet

http://hdl.handle.net/1721.1/100245
https://orcid.org/0000-0001-7215-1439

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