Detecting Kinase Activities from Single Cell Lysate Using Concentration-Enhanced Mobility Shift Assay
Electrokinetic preconcentration coupled with mobility shift assays can give rise to very high detection sensitivities. We describe a microfluidic device that utilizes this principle to detect cellular kinase activities by simultaneously concentrating and separating substrate peptides with different...
Main Authors: | Cheow, Lih Feng, Sarkar, Aniruddh, Kolitz, Sarah, Han, Jongyoon, Lauffenburger, Douglas A. |
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Other Authors: | Massachusetts Institute of Technology. Department of Biological Engineering |
Format: | Article |
Language: | en_US |
Published: |
American Chemical Society (ACS)
2015
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Online Access: | http://hdl.handle.net/1721.1/100245 https://orcid.org/0000-0001-7215-1439 |
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