Parallel and flexible fluorescent imaging using two-photon RESOLFT super-resolution microscopy with spatial light modulator control
Thesis: S.M., Massachusetts Institute of Technology, Department of Mechanical Engineering, 2015.
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| Format: | Thesis |
| Language: | eng |
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Massachusetts Institute of Technology
2015
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| Online Access: | http://hdl.handle.net/1721.1/100346 |
| _version_ | 1826209056488947712 |
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| author | Xue, Yi, S.M. Massachusetts Institute of Technology |
| author2 | Peter T. C. So. |
| author_facet | Peter T. C. So. Xue, Yi, S.M. Massachusetts Institute of Technology |
| author_sort | Xue, Yi, S.M. Massachusetts Institute of Technology |
| collection | MIT |
| description | Thesis: S.M., Massachusetts Institute of Technology, Department of Mechanical Engineering, 2015. |
| first_indexed | 2024-09-23T14:16:39Z |
| format | Thesis |
| id | mit-1721.1/100346 |
| institution | Massachusetts Institute of Technology |
| language | eng |
| last_indexed | 2024-09-23T14:16:39Z |
| publishDate | 2015 |
| publisher | Massachusetts Institute of Technology |
| record_format | dspace |
| spelling | mit-1721.1/1003462019-04-12T13:27:49Z Parallel and flexible fluorescent imaging using two-photon RESOLFT super-resolution microscopy with spatial light modulator control Xue, Yi, S.M. Massachusetts Institute of Technology Peter T. C. So. Massachusetts Institute of Technology. Department of Mechanical Engineering. Massachusetts Institute of Technology. Department of Mechanical Engineering. Mechanical Engineering. Thesis: S.M., Massachusetts Institute of Technology, Department of Mechanical Engineering, 2015. Cataloged from PDF version of thesis. Includes bibliographical references (pages 65-68). High resolution imaging in three dimension is important for biological research. The RESOLFT (Reversible Saturable Optical Fluorescence Transitions) fluorescent microscopy is one technique which can achieve lateral super-resolution imaging. Two-photon microscopy naturally generate high resolution in the longitudinal direction with less background compared to single photon excitation. We combine these two methods to realize three-dimensional high-resolution imaging. This super-resolution method also is limited in imaging speed. We use a spatial light modulator (SLM) as a flexible phase mask of the microscopy. It is used to compensate the system aberration, as well as increasing the imaging speed. The parallel scanning generates multiple super-resolution focuses as an array or in arbitrary positions by phase retrieval calculation. This microscopy combined with SLM control could applied to high throughput 3D imaging or multiple spots tracking in high-resolution. by Yi Xue. S.M. 2015-12-16T16:33:25Z 2015-12-16T16:33:25Z 2015 2015 Thesis http://hdl.handle.net/1721.1/100346 931070512 eng M.I.T. theses are protected by copyright. They may be viewed from this source for any purpose, but reproduction or distribution in any format is prohibited without written permission. See provided URL for inquiries about permission. http://dspace.mit.edu/handle/1721.1/7582 68 pages application/pdf Massachusetts Institute of Technology |
| spellingShingle | Mechanical Engineering. Xue, Yi, S.M. Massachusetts Institute of Technology Parallel and flexible fluorescent imaging using two-photon RESOLFT super-resolution microscopy with spatial light modulator control |
| title | Parallel and flexible fluorescent imaging using two-photon RESOLFT super-resolution microscopy with spatial light modulator control |
| title_full | Parallel and flexible fluorescent imaging using two-photon RESOLFT super-resolution microscopy with spatial light modulator control |
| title_fullStr | Parallel and flexible fluorescent imaging using two-photon RESOLFT super-resolution microscopy with spatial light modulator control |
| title_full_unstemmed | Parallel and flexible fluorescent imaging using two-photon RESOLFT super-resolution microscopy with spatial light modulator control |
| title_short | Parallel and flexible fluorescent imaging using two-photon RESOLFT super-resolution microscopy with spatial light modulator control |
| title_sort | parallel and flexible fluorescent imaging using two photon resolft super resolution microscopy with spatial light modulator control |
| topic | Mechanical Engineering. |
| url | http://hdl.handle.net/1721.1/100346 |
| work_keys_str_mv | AT xueyismmassachusettsinstituteoftechnology parallelandflexiblefluorescentimagingusingtwophotonresolftsuperresolutionmicroscopywithspatiallightmodulatorcontrol |