Delineation of Natural Killer Cell Differentiation from Myeloid Progenitors in Human
Understanding of natural killer (NK) cell development in human is incomplete partly because of limited access to appropriate human tissues. We have developed a cytokine-enhanced humanized mouse model with greatly improved reconstitution and function of human NK cells. Here we report the presence of...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | en_US |
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Nature Publishing Group
2015
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| Online Access: | http://hdl.handle.net/1721.1/100500 https://orcid.org/0000-0002-5687-6154 |
| _version_ | 1826189714568249344 |
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| author | Chen, Qingfeng Ye, Weijian Jian Tan, Wei Yong, Kylie Su Mei Liu, Min Qi Tan, Shu Loh, Eva TE Chang, Kenneth Chye Tan, Thiam Preiser, Peter R. Chen, Jianzhu |
| author2 | Massachusetts Institute of Technology. Department of Biology |
| author_facet | Massachusetts Institute of Technology. Department of Biology Chen, Qingfeng Ye, Weijian Jian Tan, Wei Yong, Kylie Su Mei Liu, Min Qi Tan, Shu Loh, Eva TE Chang, Kenneth Chye Tan, Thiam Preiser, Peter R. Chen, Jianzhu |
| author_sort | Chen, Qingfeng |
| collection | MIT |
| description | Understanding of natural killer (NK) cell development in human is incomplete partly because of limited access to appropriate human tissues. We have developed a cytokine-enhanced humanized mouse model with greatly improved reconstitution and function of human NK cells. Here we report the presence of a cell population in the bone marrow of the cytokine-treated humanized mice that express both NK cell marker CD56 and myeloid markers such as CD36 and CD33. The CD56[superscript +]CD33[superscript +]CD36[superscript +] cells are also found in human cord blood, fetal and adult bone marrow. Although the CD56[superscript +]CD33[superscript +]CD36[superscript +] cells do not express the common NK cell functional receptors and exhibit little cytotoxic and cytokine-producing activities, they readily differentiate into mature NK cells by acquiring expression of NK cell receptors and losing expression of the myeloid markers. Further studies show that CD33[superscript +]CD36[superscript +] myeloid NK precursors are derived from granulo-myelomonocytic progenitors. These results delineate the pathway of human NK cell differentiation from myeloid progenitors in the bone marrow and suggest the utility of humanized mice for studying human hematopoiesis. |
| first_indexed | 2024-09-23T08:19:53Z |
| format | Article |
| id | mit-1721.1/100500 |
| institution | Massachusetts Institute of Technology |
| language | en_US |
| last_indexed | 2024-09-23T08:19:53Z |
| publishDate | 2015 |
| publisher | Nature Publishing Group |
| record_format | dspace |
| spelling | mit-1721.1/1005002022-09-30T09:04:46Z Delineation of Natural Killer Cell Differentiation from Myeloid Progenitors in Human Chen, Qingfeng Ye, Weijian Jian Tan, Wei Yong, Kylie Su Mei Liu, Min Qi Tan, Shu Loh, Eva TE Chang, Kenneth Chye Tan, Thiam Preiser, Peter R. Chen, Jianzhu Massachusetts Institute of Technology. Department of Biology Koch Institute for Integrative Cancer Research at MIT Chen, Jianzhu Understanding of natural killer (NK) cell development in human is incomplete partly because of limited access to appropriate human tissues. We have developed a cytokine-enhanced humanized mouse model with greatly improved reconstitution and function of human NK cells. Here we report the presence of a cell population in the bone marrow of the cytokine-treated humanized mice that express both NK cell marker CD56 and myeloid markers such as CD36 and CD33. The CD56[superscript +]CD33[superscript +]CD36[superscript +] cells are also found in human cord blood, fetal and adult bone marrow. Although the CD56[superscript +]CD33[superscript +]CD36[superscript +] cells do not express the common NK cell functional receptors and exhibit little cytotoxic and cytokine-producing activities, they readily differentiate into mature NK cells by acquiring expression of NK cell receptors and losing expression of the myeloid markers. Further studies show that CD33[superscript +]CD36[superscript +] myeloid NK precursors are derived from granulo-myelomonocytic progenitors. These results delineate the pathway of human NK cell differentiation from myeloid progenitors in the bone marrow and suggest the utility of humanized mice for studying human hematopoiesis. 2015-12-23T16:17:49Z 2015-12-23T16:17:49Z 2015-10 2015-07 Article http://purl.org/eprint/type/JournalArticle 2045-2322 http://hdl.handle.net/1721.1/100500 Chen, Qingfeng, Weijian Ye, Wei Jian Tan, Kylie Su Mei Yong, Min Liu, Shu Qi Tan, Eva Loh, et al. “Delineation of Natural Killer Cell Differentiation from Myeloid Progenitors in Human.” Scientific Reports 5 (October 12, 2015): 15118. https://orcid.org/0000-0002-5687-6154 en_US http://dx.doi.org/10.1038/srep15118 Scientific Reports Creative Commons Attribution http://creativecommons.org/licenses/by/4.0/ application/pdf Nature Publishing Group Nature Publishing Group |
| spellingShingle | Chen, Qingfeng Ye, Weijian Jian Tan, Wei Yong, Kylie Su Mei Liu, Min Qi Tan, Shu Loh, Eva TE Chang, Kenneth Chye Tan, Thiam Preiser, Peter R. Chen, Jianzhu Delineation of Natural Killer Cell Differentiation from Myeloid Progenitors in Human |
| title | Delineation of Natural Killer Cell Differentiation from Myeloid Progenitors in Human |
| title_full | Delineation of Natural Killer Cell Differentiation from Myeloid Progenitors in Human |
| title_fullStr | Delineation of Natural Killer Cell Differentiation from Myeloid Progenitors in Human |
| title_full_unstemmed | Delineation of Natural Killer Cell Differentiation from Myeloid Progenitors in Human |
| title_short | Delineation of Natural Killer Cell Differentiation from Myeloid Progenitors in Human |
| title_sort | delineation of natural killer cell differentiation from myeloid progenitors in human |
| url | http://hdl.handle.net/1721.1/100500 https://orcid.org/0000-0002-5687-6154 |
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