A novel α-hemolytic Streptococcus species (Streptococcus azizii sp. nov.) associated with meningoencephalitis in naïve weanling C57BL/6 mice
During 1 year, experimentally naïve C57BL/6NCrl weanlings born to timed-pregnant dams from a single vendor demonstrated markedly increased mortality associated with runting, abnormal gait, and decreased activity. Gram-positive, aerobic, α-hemolytic, coccoid bacteria were isolated from the meninges (...
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| Format: | Article |
| Language: | en_US |
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American Association for Laboratory Animal Science
2015
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| Online Access: | http://hdl.handle.net/1721.1/100517 https://orcid.org/0000-0001-9307-6116 |
| _version_ | 1826210832577462272 |
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| author | Braden, Gillian C. Arbona, Rodolfo Ricart Lepherd, Michelle Monette, Sebastien Toma, Aziz Fox, James G. Dewhirst, Floyd E. Lipman, Neil S. |
| author2 | Massachusetts Institute of Technology. Department of Biological Engineering |
| author_facet | Massachusetts Institute of Technology. Department of Biological Engineering Braden, Gillian C. Arbona, Rodolfo Ricart Lepherd, Michelle Monette, Sebastien Toma, Aziz Fox, James G. Dewhirst, Floyd E. Lipman, Neil S. |
| author_sort | Braden, Gillian C. |
| collection | MIT |
| description | During 1 year, experimentally naïve C57BL/6NCrl weanlings born to timed-pregnant dams from a single vendor demonstrated markedly increased mortality associated with runting, abnormal gait, and decreased activity. Gram-positive, aerobic, α-hemolytic, coccoid bacteria were isolated from the meninges (n = 16), blood (n = 1), and kidneys (n = 1) of clinically affected weanlings (n = 15); from the uterus (n = 1), meninges (n = 1), and oral cavity (n = 2) of 3 dams; and from the meninges and oral cavity of a clinically affected 86-d-old mouse in the same colony. Multifocal, necrosuppurative meningoencephalitis and ventriculitis with intralesional gram-positive coccoid bacteria were present in all but 2 affected animals. The bacterium also was isolated from the oral cavity of an asymptomatic timed-pregnant dam (1 of 23) from the same vendor and from 8 mice at the vendor's facility. All isolates (n = 25) were identified by using 2 semiautomated rapid-identification systems, one of which consistently identified the causative bacterium as Aerococcus viridans 2 (n = 12) or 3 (n = 13), with probabilities of 55.7% to 98.3%. The bacterium did not grow in 6.5% NaCl at 10 °C, thus suggesting a Streptococcus species. Partial 16S rRNA sequencing of 4 isolates suggested S. hyointestinalis (probability, 93.4%) and S. gallinaceus (99.5%). Full 16S rRNA sequences for 3 isolates identified the bacterium as a novel Streptococcus species most closely related to S. acidominimus strain LGM (96.5%) and Streptococcus species strain Smarlab 3301444 (96.3%) and for which we propose the name S. azizii. |
| first_indexed | 2024-09-23T14:56:08Z |
| format | Article |
| id | mit-1721.1/100517 |
| institution | Massachusetts Institute of Technology |
| language | en_US |
| last_indexed | 2024-09-23T14:56:08Z |
| publishDate | 2015 |
| publisher | American Association for Laboratory Animal Science |
| record_format | dspace |
| spelling | mit-1721.1/1005172022-10-01T23:28:35Z A novel α-hemolytic Streptococcus species (Streptococcus azizii sp. nov.) associated with meningoencephalitis in naïve weanling C57BL/6 mice Braden, Gillian C. Arbona, Rodolfo Ricart Lepherd, Michelle Monette, Sebastien Toma, Aziz Fox, James G. Dewhirst, Floyd E. Lipman, Neil S. Massachusetts Institute of Technology. Department of Biological Engineering Fox, James G. Fox, James G. During 1 year, experimentally naïve C57BL/6NCrl weanlings born to timed-pregnant dams from a single vendor demonstrated markedly increased mortality associated with runting, abnormal gait, and decreased activity. Gram-positive, aerobic, α-hemolytic, coccoid bacteria were isolated from the meninges (n = 16), blood (n = 1), and kidneys (n = 1) of clinically affected weanlings (n = 15); from the uterus (n = 1), meninges (n = 1), and oral cavity (n = 2) of 3 dams; and from the meninges and oral cavity of a clinically affected 86-d-old mouse in the same colony. Multifocal, necrosuppurative meningoencephalitis and ventriculitis with intralesional gram-positive coccoid bacteria were present in all but 2 affected animals. The bacterium also was isolated from the oral cavity of an asymptomatic timed-pregnant dam (1 of 23) from the same vendor and from 8 mice at the vendor's facility. All isolates (n = 25) were identified by using 2 semiautomated rapid-identification systems, one of which consistently identified the causative bacterium as Aerococcus viridans 2 (n = 12) or 3 (n = 13), with probabilities of 55.7% to 98.3%. The bacterium did not grow in 6.5% NaCl at 10 °C, thus suggesting a Streptococcus species. Partial 16S rRNA sequencing of 4 isolates suggested S. hyointestinalis (probability, 93.4%) and S. gallinaceus (99.5%). Full 16S rRNA sequences for 3 isolates identified the bacterium as a novel Streptococcus species most closely related to S. acidominimus strain LGM (96.5%) and Streptococcus species strain Smarlab 3301444 (96.3%) and for which we propose the name S. azizii. 2015-12-28T00:31:01Z 2015-12-28T00:31:01Z 2015-06 2014-12 Article http://purl.org/eprint/type/JournalArticle 1532-0820 http://hdl.handle.net/1721.1/100517 Braden, Gillian C., Rodolfo Ricart Arbona, Michelle Lepherd, Sebastien Monette, Aziz Toma, James G. Fox, Floyd E. Dewhirst, and Neil S. Lipman. "A novel α-hemolytic Streptococcus species (Streptococcus azizii sp. nov.) associated with meningoencephalitis in naïve weanling C57BL/6 mice." Comparative Medicine, Volume 65, Number 3 (June 2015). https://orcid.org/0000-0001-9307-6116 en_US http://www.ingentaconnect.com/content/aalas/cm/2015/00000065/00000003/art00004?token=00491146b7e2a46762c6b66217d703c70533a3e5f7a673f7b2f27375f2a72752d70f1a671 Comparative Medicine Creative Commons Attribution-Noncommercial-Share Alike http://creativecommons.org/licenses/by-nc-sa/4.0/ application/pdf American Association for Laboratory Animal Science Prof. Fox via Howard Silver |
| spellingShingle | Braden, Gillian C. Arbona, Rodolfo Ricart Lepherd, Michelle Monette, Sebastien Toma, Aziz Fox, James G. Dewhirst, Floyd E. Lipman, Neil S. A novel α-hemolytic Streptococcus species (Streptococcus azizii sp. nov.) associated with meningoencephalitis in naïve weanling C57BL/6 mice |
| title | A novel α-hemolytic Streptococcus species (Streptococcus azizii sp. nov.) associated with meningoencephalitis in naïve weanling C57BL/6 mice |
| title_full | A novel α-hemolytic Streptococcus species (Streptococcus azizii sp. nov.) associated with meningoencephalitis in naïve weanling C57BL/6 mice |
| title_fullStr | A novel α-hemolytic Streptococcus species (Streptococcus azizii sp. nov.) associated with meningoencephalitis in naïve weanling C57BL/6 mice |
| title_full_unstemmed | A novel α-hemolytic Streptococcus species (Streptococcus azizii sp. nov.) associated with meningoencephalitis in naïve weanling C57BL/6 mice |
| title_short | A novel α-hemolytic Streptococcus species (Streptococcus azizii sp. nov.) associated with meningoencephalitis in naïve weanling C57BL/6 mice |
| title_sort | novel α hemolytic streptococcus species streptococcus azizii sp nov associated with meningoencephalitis in naive weanling c57bl 6 mice |
| url | http://hdl.handle.net/1721.1/100517 https://orcid.org/0000-0001-9307-6116 |
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