Sequence determinants of improved CRISPR sgRNA design

Sequence determinants of improved CRISPR sgRNA design

The CRISPR/Cas9 system has revolutionized mammalian somatic cell genetics. Genome-wide functional screens using CRISPR/Cas9-mediated knockout or dCas9 fusion-mediated inhibition/activation (CRISPRi/a) are powerful techniques for discovering phenotype-associated gene function. We systematically asses...

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Detalhes bibliográficos
Main Authors: Xu, Han, Xiao, Tengfei, Chen, Chen-Hao, Li, Wei, Meyer, Clifford A., Wu, Qiu, Wu, Di, Cong, Le, Zhang, Feng, Liu, Jun S., Brown, Myles, Liu, X. Shirley
Outros Autores: Massachusetts Institute of Technology. Department of Biological Engineering
Formato: Artigo
Idioma:en_US
Publicado em: Cold Spring Harbor Laboratory Press 2016
Acesso em linha:http://hdl.handle.net/1721.1/100787
https://orcid.org/0000-0003-2782-2509

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