Deformability of Tumor Cells versus Blood Cells
The potential for circulating tumor cells (CTCs) to elucidate the process of cancer metastasis and inform clinical decision-making has made their isolation of great importance. However, CTCs are rare in the blood, and universal properties with which to identify them remain elusive. As technological...
| Main Authors: | , , , , , , , , , |
|---|---|
| Other Authors: | |
| Format: | Article |
| Language: | en_US |
| Published: |
Nature Publishing Group
2016
|
| Online Access: | http://hdl.handle.net/1721.1/100904 https://orcid.org/0000-0001-5223-9433 https://orcid.org/0000-0003-3415-3614 https://orcid.org/0000-0003-4110-1388 https://orcid.org/0000-0001-7603-8396 |
| _version_ | 1811083012279894016 |
|---|---|
| author | Byun, Sangwon Begum, Shahinoor Miyamoto, David T. Maheswaran, Shyamala Stott, Shannon L. Toner, Mehmet Hecht, Vivian Chaya Hynes, Richard O Manalis, Scott R Shaw, Josephine |
| author2 | Massachusetts Institute of Technology. Department of Biological Engineering |
| author_facet | Massachusetts Institute of Technology. Department of Biological Engineering Byun, Sangwon Begum, Shahinoor Miyamoto, David T. Maheswaran, Shyamala Stott, Shannon L. Toner, Mehmet Hecht, Vivian Chaya Hynes, Richard O Manalis, Scott R Shaw, Josephine |
| author_sort | Byun, Sangwon |
| collection | MIT |
| description | The potential for circulating tumor cells (CTCs) to elucidate the process of cancer metastasis and inform clinical decision-making has made their isolation of great importance. However, CTCs are rare in the blood, and universal properties with which to identify them remain elusive. As technological advancements have made single-cell deformability measurements increasingly routine, the assessment of physical distinctions between tumor cells and blood cells may provide insight into the feasibility of deformability-based methods for identifying CTCs in patient blood. To this end, we present an initial study assessing deformability differences between tumor cells and blood cells, indicated by the length of time required for them to pass through a microfluidic constriction. Here, we demonstrate that deformability changes in tumor cells that have undergone phenotypic shifts are small compared to differences between tumor cell lines and blood cells. Additionally, in a syngeneic mouse tumor model, cells that are able to exit a tumor and enter circulation are not required to be more deformable than the cells that were first injected into the mouse. However, a limited study of metastatic prostate cancer patients provides evidence that some CTCs may be more mechanically similar to blood cells than to typical tumor cell lines. |
| first_indexed | 2024-09-23T12:17:51Z |
| format | Article |
| id | mit-1721.1/100904 |
| institution | Massachusetts Institute of Technology |
| language | en_US |
| last_indexed | 2024-09-23T12:17:51Z |
| publishDate | 2016 |
| publisher | Nature Publishing Group |
| record_format | dspace |
| spelling | mit-1721.1/1009042022-10-01T08:55:26Z Deformability of Tumor Cells versus Blood Cells Byun, Sangwon Begum, Shahinoor Miyamoto, David T. Maheswaran, Shyamala Stott, Shannon L. Toner, Mehmet Hecht, Vivian Chaya Hynes, Richard O Manalis, Scott R Shaw, Josephine Massachusetts Institute of Technology. Department of Biological Engineering Massachusetts Institute of Technology. Department of Biology Massachusetts Institute of Technology. Department of Mechanical Engineering Koch Institute for Integrative Cancer Research at MIT Bagnall, Josephine Byun, Sangwon Begum, Shahinoor Hecht, Vivian Chaya Hynes, Richard O. Manalis, Scott R. The potential for circulating tumor cells (CTCs) to elucidate the process of cancer metastasis and inform clinical decision-making has made their isolation of great importance. However, CTCs are rare in the blood, and universal properties with which to identify them remain elusive. As technological advancements have made single-cell deformability measurements increasingly routine, the assessment of physical distinctions between tumor cells and blood cells may provide insight into the feasibility of deformability-based methods for identifying CTCs in patient blood. To this end, we present an initial study assessing deformability differences between tumor cells and blood cells, indicated by the length of time required for them to pass through a microfluidic constriction. Here, we demonstrate that deformability changes in tumor cells that have undergone phenotypic shifts are small compared to differences between tumor cell lines and blood cells. Additionally, in a syngeneic mouse tumor model, cells that are able to exit a tumor and enter circulation are not required to be more deformable than the cells that were first injected into the mouse. However, a limited study of metastatic prostate cancer patients provides evidence that some CTCs may be more mechanically similar to blood cells than to typical tumor cell lines. Janssen Pharmaceutical Ltd. National Cancer Institute (U.S.). Physical Sciences Oncology Center (U54CA143874) MIT-Harvard Center of Cancer Nanotechnology Excellence (Grant 26697290-47281-A) Stand Up To Cancer National Institutes of Health (U.S.). P41 Biotechnology Resource Center National Cancer Institute (U.S.) (Koch Institute Support Grant P30-CA14051) 2016-01-18T22:53:41Z 2016-01-18T22:53:41Z 2015-12 2015-07 Article http://purl.org/eprint/type/JournalArticle 2045-2322 http://hdl.handle.net/1721.1/100904 Shaw Bagnall, Josephine, Sangwon Byun, Shahinoor Begum, David T. Miyamoto, Vivian C. Hecht, Shyamala Maheswaran, Shannon L. Stott, Mehmet Toner, Richard O. Hynes, and Scott R. Manalis. “Deformability of Tumor Cells Versus Blood Cells.” Scientific Reports 5 (December 18, 2015): 18542. https://orcid.org/0000-0001-5223-9433 https://orcid.org/0000-0003-3415-3614 https://orcid.org/0000-0003-4110-1388 https://orcid.org/0000-0001-7603-8396 en_US http://dx.doi.org/10.1038/srep18542 Scientific Reports Creative Commons Attribution http://creativecommons.org/licenses/by/4.0/ application/pdf Nature Publishing Group Nature Publishing Group |
| spellingShingle | Byun, Sangwon Begum, Shahinoor Miyamoto, David T. Maheswaran, Shyamala Stott, Shannon L. Toner, Mehmet Hecht, Vivian Chaya Hynes, Richard O Manalis, Scott R Shaw, Josephine Deformability of Tumor Cells versus Blood Cells |
| title | Deformability of Tumor Cells versus Blood Cells |
| title_full | Deformability of Tumor Cells versus Blood Cells |
| title_fullStr | Deformability of Tumor Cells versus Blood Cells |
| title_full_unstemmed | Deformability of Tumor Cells versus Blood Cells |
| title_short | Deformability of Tumor Cells versus Blood Cells |
| title_sort | deformability of tumor cells versus blood cells |
| url | http://hdl.handle.net/1721.1/100904 https://orcid.org/0000-0001-5223-9433 https://orcid.org/0000-0003-3415-3614 https://orcid.org/0000-0003-4110-1388 https://orcid.org/0000-0001-7603-8396 |
| work_keys_str_mv | AT byunsangwon deformabilityoftumorcellsversusbloodcells AT begumshahinoor deformabilityoftumorcellsversusbloodcells AT miyamotodavidt deformabilityoftumorcellsversusbloodcells AT maheswaranshyamala deformabilityoftumorcellsversusbloodcells AT stottshannonl deformabilityoftumorcellsversusbloodcells AT tonermehmet deformabilityoftumorcellsversusbloodcells AT hechtvivianchaya deformabilityoftumorcellsversusbloodcells AT hynesrichardo deformabilityoftumorcellsversusbloodcells AT manalisscottr deformabilityoftumorcellsversusbloodcells AT shawjosephine deformabilityoftumorcellsversusbloodcells |