Cell-Based Biosensor to Report DNA Damage in Micro- and Nanosystems
Understanding how newly engineered micro- and nanoscale materials and systems that interact with cells impact cell physiology is crucial for the development and ultimate adoption of such technologies. Reports regarding the genotoxic impact of forces applied to cells in such systems that can both dir...
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| Format: | Article |
| Language: | en_US |
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American Chemical Society (ACS)
2016
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| Online Access: | http://hdl.handle.net/1721.1/101081 https://orcid.org/0000-0001-8898-2296 https://orcid.org/0000-0002-3690-519X |
| _version_ | 1826196718051393536 |
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| author | Fendyur, Anna Varma, Sarvesh Lo, Catherine T. Voldman, Joel |
| author2 | Massachusetts Institute of Technology. Department of Electrical Engineering and Computer Science |
| author_facet | Massachusetts Institute of Technology. Department of Electrical Engineering and Computer Science Fendyur, Anna Varma, Sarvesh Lo, Catherine T. Voldman, Joel |
| author_sort | Fendyur, Anna |
| collection | MIT |
| description | Understanding how newly engineered micro- and nanoscale materials and systems that interact with cells impact cell physiology is crucial for the development and ultimate adoption of such technologies. Reports regarding the genotoxic impact of forces applied to cells in such systems that can both directly or indirectly damage DNA emphasize the need for developing facile methods to assess how materials and technologies affect cell physiology. To address this need we have developed a TurboRFP-based DNA damage reporter cell line in NIH-3T3 cells that fluoresce to report genotoxic stress caused by a wide variety of agents, from chemical genotoxic agents to UV-C radiation. Our biosensor was successfully implemented in reporting the genotoxic impact of nanomaterials, demonstrating the ability to assess size dependent geno- and cyto-toxicity. The biosensor cells can be assayed in a high throughput, noninvasive manner, with no need for overly sophisticated equipment or additional reagents. We believe that this open-source biosensor is an important resource for the community of micro- and nanomaterials and systems designers and users who wish to evaluate the impact of systems and materials on cell physiology. |
| first_indexed | 2024-09-23T10:36:38Z |
| format | Article |
| id | mit-1721.1/101081 |
| institution | Massachusetts Institute of Technology |
| language | en_US |
| last_indexed | 2024-09-23T10:36:38Z |
| publishDate | 2016 |
| publisher | American Chemical Society (ACS) |
| record_format | dspace |
| spelling | mit-1721.1/1010812022-09-27T10:05:14Z Cell-Based Biosensor to Report DNA Damage in Micro- and Nanosystems Fendyur, Anna Varma, Sarvesh Lo, Catherine T. Voldman, Joel Massachusetts Institute of Technology. Department of Electrical Engineering and Computer Science Fendyur, Anna Varma, Sarvesh Lo, Catherine T. Voldman, Joel Understanding how newly engineered micro- and nanoscale materials and systems that interact with cells impact cell physiology is crucial for the development and ultimate adoption of such technologies. Reports regarding the genotoxic impact of forces applied to cells in such systems that can both directly or indirectly damage DNA emphasize the need for developing facile methods to assess how materials and technologies affect cell physiology. To address this need we have developed a TurboRFP-based DNA damage reporter cell line in NIH-3T3 cells that fluoresce to report genotoxic stress caused by a wide variety of agents, from chemical genotoxic agents to UV-C radiation. Our biosensor was successfully implemented in reporting the genotoxic impact of nanomaterials, demonstrating the ability to assess size dependent geno- and cyto-toxicity. The biosensor cells can be assayed in a high throughput, noninvasive manner, with no need for overly sophisticated equipment or additional reagents. We believe that this open-source biosensor is an important resource for the community of micro- and nanomaterials and systems designers and users who wish to evaluate the impact of systems and materials on cell physiology. National Institutes of Health (U.S.) (Grant GM090194) 2016-02-03T16:46:27Z 2016-02-03T16:46:27Z 2014-07 2014-04 Article http://purl.org/eprint/type/JournalArticle 0003-2700 1520-6882 http://hdl.handle.net/1721.1/101081 Fendyur, Anna, Sarvesh Varma, Catherine T. Lo, and Joel Voldman. “Cell-Based Biosensor to Report DNA Damage in Micro- and Nanosystems.” Analytical Chemistry 86, no. 15 (August 5, 2014): 7598–7605. https://orcid.org/0000-0001-8898-2296 https://orcid.org/0000-0002-3690-519X en_US http://dx.doi.org/10.1021/ac501412c Analytical Chemistry Article is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use. application/pdf American Chemical Society (ACS) ACS |
| spellingShingle | Fendyur, Anna Varma, Sarvesh Lo, Catherine T. Voldman, Joel Cell-Based Biosensor to Report DNA Damage in Micro- and Nanosystems |
| title | Cell-Based Biosensor to Report DNA Damage in Micro- and Nanosystems |
| title_full | Cell-Based Biosensor to Report DNA Damage in Micro- and Nanosystems |
| title_fullStr | Cell-Based Biosensor to Report DNA Damage in Micro- and Nanosystems |
| title_full_unstemmed | Cell-Based Biosensor to Report DNA Damage in Micro- and Nanosystems |
| title_short | Cell-Based Biosensor to Report DNA Damage in Micro- and Nanosystems |
| title_sort | cell based biosensor to report dna damage in micro and nanosystems |
| url | http://hdl.handle.net/1721.1/101081 https://orcid.org/0000-0001-8898-2296 https://orcid.org/0000-0002-3690-519X |
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