Engineering Escherichia coli coculture systems for the production of biochemical products
Engineering microbial consortia to express complex biosynthetic pathways efficiently for the production of valuable compounds is a promising approach for metabolic engineering and synthetic biology. Here, we report the design, optimization, and scale-up of an Escherichia coli-E. coli coculture that...
| Main Authors: | , , , |
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| Other Authors: | |
| Format: | Article |
| Language: | en_US |
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National Academy of Sciences (U.S.)
2016
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| Online Access: | http://hdl.handle.net/1721.1/101117 https://orcid.org/0000-0002-7059-572X https://orcid.org/0000-0001-6909-4568 |
| _version_ | 1811074194392219648 |
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| author | Zhang, Haoran Li, Zhengjun Stephanopoulos, Gregory Pereira, Brian J. |
| author2 | Massachusetts Institute of Technology. Department of Chemical Engineering |
| author_facet | Massachusetts Institute of Technology. Department of Chemical Engineering Zhang, Haoran Li, Zhengjun Stephanopoulos, Gregory Pereira, Brian J. |
| author_sort | Zhang, Haoran |
| collection | MIT |
| description | Engineering microbial consortia to express complex biosynthetic pathways efficiently for the production of valuable compounds is a promising approach for metabolic engineering and synthetic biology. Here, we report the design, optimization, and scale-up of an Escherichia coli-E. coli coculture that successfully overcomes fundamental microbial production limitations, such as high-level intermediate secretion and low-efficiency sugar mixture utilization. For the production of the important chemical cis,cis-muconic acid, we show that the coculture approach achieves a production yield of 0.35 g/g from a glucose/xylose mixture, which is significantly higher than reported in previous reports. By efficiently producing another compound, 4-hydroxybenzoic acid, we also demonstrate that the approach is generally applicable for biosynthesis of other important industrial products. |
| first_indexed | 2024-09-23T09:45:26Z |
| format | Article |
| id | mit-1721.1/101117 |
| institution | Massachusetts Institute of Technology |
| language | en_US |
| last_indexed | 2024-09-23T09:45:26Z |
| publishDate | 2016 |
| publisher | National Academy of Sciences (U.S.) |
| record_format | dspace |
| spelling | mit-1721.1/1011172022-09-26T13:31:40Z Engineering Escherichia coli coculture systems for the production of biochemical products Zhang, Haoran Li, Zhengjun Stephanopoulos, Gregory Pereira, Brian J. Massachusetts Institute of Technology. Department of Chemical Engineering Zhang, Haoran Pereira, Brian J. Li, Zhengjun Stephanopoulos, Gregory Engineering microbial consortia to express complex biosynthetic pathways efficiently for the production of valuable compounds is a promising approach for metabolic engineering and synthetic biology. Here, we report the design, optimization, and scale-up of an Escherichia coli-E. coli coculture that successfully overcomes fundamental microbial production limitations, such as high-level intermediate secretion and low-efficiency sugar mixture utilization. For the production of the important chemical cis,cis-muconic acid, we show that the coculture approach achieves a production yield of 0.35 g/g from a glucose/xylose mixture, which is significantly higher than reported in previous reports. By efficiently producing another compound, 4-hydroxybenzoic acid, we also demonstrate that the approach is generally applicable for biosynthesis of other important industrial products. United States. Dept. of Energy (Grant DE-SC0008744) 2016-02-05T14:49:54Z 2016-02-05T14:49:54Z 2015-07 2015-04 Article http://purl.org/eprint/type/JournalArticle 0027-8424 1091-6490 http://hdl.handle.net/1721.1/101117 Zhang, Haoran, Brian Pereira, Zhengjun Li, and Gregory Stephanopoulos. “Engineering Escherichia Coli Coculture Systems for the Production of Biochemical Products.” Proc Natl Acad Sci USA 112, no. 27 (June 25, 2015): 8266–8271. https://orcid.org/0000-0002-7059-572X https://orcid.org/0000-0001-6909-4568 en_US http://dx.doi.org/10.1073/pnas.1506781112 Proceedings of the National Academy of Sciences Article is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use. application/pdf National Academy of Sciences (U.S.) National Academy of Sciences (U.S.) |
| spellingShingle | Zhang, Haoran Li, Zhengjun Stephanopoulos, Gregory Pereira, Brian J. Engineering Escherichia coli coculture systems for the production of biochemical products |
| title | Engineering Escherichia coli coculture systems for the production of biochemical products |
| title_full | Engineering Escherichia coli coculture systems for the production of biochemical products |
| title_fullStr | Engineering Escherichia coli coculture systems for the production of biochemical products |
| title_full_unstemmed | Engineering Escherichia coli coculture systems for the production of biochemical products |
| title_short | Engineering Escherichia coli coculture systems for the production of biochemical products |
| title_sort | engineering escherichia coli coculture systems for the production of biochemical products |
| url | http://hdl.handle.net/1721.1/101117 https://orcid.org/0000-0002-7059-572X https://orcid.org/0000-0001-6909-4568 |
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