Multilayer Films Assembled from Naturally-Derived Materials for Controlled Protein Release
Herein we designed and characterized films composed of naturally derived materials for controlled release of proteins. Traditional drug delivery strategies rely on synthetic or semisynthetic materials or utilize potentially denaturing assembly conditions that are not optimal for sensitive biologics....
| Main Authors: | , , , , , , , |
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| Other Authors: | |
| Format: | Article |
| Language: | en_US |
| Published: |
American Chemical Society (ACS)
2016
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| Online Access: | http://hdl.handle.net/1721.1/101181 |
| _version_ | 1826212449442856960 |
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| author | Veselinovic, Jovana O’Neill, Nicholas Holler, Eggehard Ljubimova, Julia Y. Hammond, Paula T. Hsu, Bryan Boen Hagerman, Samantha R. Jamieson, Kelsey S. |
| author2 | Massachusetts Institute of Technology. Institute for Soldier Nanotechnologies |
| author_facet | Massachusetts Institute of Technology. Institute for Soldier Nanotechnologies Veselinovic, Jovana O’Neill, Nicholas Holler, Eggehard Ljubimova, Julia Y. Hammond, Paula T. Hsu, Bryan Boen Hagerman, Samantha R. Jamieson, Kelsey S. |
| author_sort | Veselinovic, Jovana |
| collection | MIT |
| description | Herein we designed and characterized films composed of naturally derived materials for controlled release of proteins. Traditional drug delivery strategies rely on synthetic or semisynthetic materials or utilize potentially denaturing assembly conditions that are not optimal for sensitive biologics. Layer-by-layer (LbL) assembly of films uses benign conditions and can generate films with various release mechanisms including hydrolysis-facilitated degradation. These use components such as synthetic polycations that degrade into non-natural products. Herein we report the use of a naturally derived, biocompatible and degradable polyanion, poly(β-l-malic acid), alone and in combination with chitosan in an LbL film, whose degradation products of malic acid and chitosan are both generally recognized as safe (GRAS) by the FDA. We have found that films based on this polyanion have shown sustained release of a model protein, lysozyme that can be timed from tens of minutes to multiple days through different film architectures. We also report the incorporation and release of a clinically used biologic, basic fibroblast growth factor (bFGF), which demonstrates the use of this strategy as a platform for controlled release of various biologics. |
| first_indexed | 2024-09-23T15:22:02Z |
| format | Article |
| id | mit-1721.1/101181 |
| institution | Massachusetts Institute of Technology |
| language | en_US |
| last_indexed | 2024-09-23T15:22:02Z |
| publishDate | 2016 |
| publisher | American Chemical Society (ACS) |
| record_format | dspace |
| spelling | mit-1721.1/1011812022-09-29T14:30:54Z Multilayer Films Assembled from Naturally-Derived Materials for Controlled Protein Release Veselinovic, Jovana O’Neill, Nicholas Holler, Eggehard Ljubimova, Julia Y. Hammond, Paula T. Hsu, Bryan Boen Hagerman, Samantha R. Jamieson, Kelsey S. Massachusetts Institute of Technology. Institute for Soldier Nanotechnologies Massachusetts Institute of Technology. Department of Chemical Engineering Massachusetts Institute of Technology. Department of Chemistry Hsu, Bryan Boen Hagerman, Samantha R. Jamieson, Kelsey S. Veselinovic, Jovana Hammond, Paula T. Herein we designed and characterized films composed of naturally derived materials for controlled release of proteins. Traditional drug delivery strategies rely on synthetic or semisynthetic materials or utilize potentially denaturing assembly conditions that are not optimal for sensitive biologics. Layer-by-layer (LbL) assembly of films uses benign conditions and can generate films with various release mechanisms including hydrolysis-facilitated degradation. These use components such as synthetic polycations that degrade into non-natural products. Herein we report the use of a naturally derived, biocompatible and degradable polyanion, poly(β-l-malic acid), alone and in combination with chitosan in an LbL film, whose degradation products of malic acid and chitosan are both generally recognized as safe (GRAS) by the FDA. We have found that films based on this polyanion have shown sustained release of a model protein, lysozyme that can be timed from tens of minutes to multiple days through different film architectures. We also report the incorporation and release of a clinically used biologic, basic fibroblast growth factor (bFGF), which demonstrates the use of this strategy as a platform for controlled release of various biologics. United States. Army Research Office (Contract W911NF-13-D-0001) United States. Air Force (Contract W911NF-07-D-0004) National Institutes of Health (U.S.) (National Cancer Institute (U.S.) U01 CA151815) 2016-02-12T21:23:27Z 2016-02-12T21:23:27Z 2014-05 2014-05 Article http://purl.org/eprint/type/JournalArticle 1525-7797 1526-4602 http://hdl.handle.net/1721.1/101181 Hsu, Bryan B., Samantha R Hagerman, Kelsey Jamieson, Jovana Veselinovic, Nicholas O’Neill, Eggehard Holler, Julia Y. Ljubimova, and Paula T. Hammond. “Multilayer Films Assembled from Naturally-Derived Materials for Controlled Protein Release.” Biomacromolecules 15, no. 6 (June 9, 2014): 2049–2057. en_US http://dx.doi.org/10.1021/bm5001839 Biomacromolecules Article is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use. application/pdf American Chemical Society (ACS) PMC |
| spellingShingle | Veselinovic, Jovana O’Neill, Nicholas Holler, Eggehard Ljubimova, Julia Y. Hammond, Paula T. Hsu, Bryan Boen Hagerman, Samantha R. Jamieson, Kelsey S. Multilayer Films Assembled from Naturally-Derived Materials for Controlled Protein Release |
| title | Multilayer Films Assembled from Naturally-Derived Materials for Controlled Protein Release |
| title_full | Multilayer Films Assembled from Naturally-Derived Materials for Controlled Protein Release |
| title_fullStr | Multilayer Films Assembled from Naturally-Derived Materials for Controlled Protein Release |
| title_full_unstemmed | Multilayer Films Assembled from Naturally-Derived Materials for Controlled Protein Release |
| title_short | Multilayer Films Assembled from Naturally-Derived Materials for Controlled Protein Release |
| title_sort | multilayer films assembled from naturally derived materials for controlled protein release |
| url | http://hdl.handle.net/1721.1/101181 |
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