Clotting Mimicry from Robust Hemostatic Bandages Based on Self-Assembling Peptides
Uncontrolled bleeding from traumatic wounds is a major factor in deaths resulting from military conflict, accidents, disasters and crime. Self-assembling peptide nanofibers have shown superior hemostatic activity, and herein, we elucidate their mechanism by visualizing the formation of nanofiber-bas...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | en_US |
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American Chemical Society (ACS)
2016
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| Online Access: | http://hdl.handle.net/1721.1/101197 |
| _version_ | 1826192006387335168 |
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| author | Tschabrunn, Cory M. Mehta, Manav Perez-Cuevas, Monica B. Zhang, Shuguang Hsu, Bryan Boen Conway, William E. Hammond, Paula T |
| author2 | Massachusetts Institute of Technology. Institute for Soldier Nanotechnologies |
| author_facet | Massachusetts Institute of Technology. Institute for Soldier Nanotechnologies Tschabrunn, Cory M. Mehta, Manav Perez-Cuevas, Monica B. Zhang, Shuguang Hsu, Bryan Boen Conway, William E. Hammond, Paula T |
| author_sort | Tschabrunn, Cory M. |
| collection | MIT |
| description | Uncontrolled bleeding from traumatic wounds is a major factor in deaths resulting from military conflict, accidents, disasters and crime. Self-assembling peptide nanofibers have shown superior hemostatic activity, and herein, we elucidate their mechanism by visualizing the formation of nanofiber-based clots that aggregate blood components with a similar morphology to fibrin-based clots. Furthermore, to enhance its direct application to a wound, we developed layer-by-layer assembled thin film coatings onto common materials used for wound dressings—gauze and gelatin sponges. We find these nanofibers elute upon hydration under physiological conditions and generate nanofiber-based clots with blood. After exposure to a range of harsh temperature conditions (−80 to 60 °C) for a week and even 5 months at 60 °C, these hemostatic bandages remain capable of releasing active nanofibers. In addition, the application of these nanofiber-based films from gauze bandages was found to accelerate hemostasis in porcine skin wounds as compared to plain gauze. The thermal robustness, in combination with the self-assembling peptide’s potent hemostatic activity, biocompatibility, biodegradability, and low cost of production, makes this a promising approach for a cheap yet effective hemostatic bandage. |
| first_indexed | 2024-09-23T09:04:44Z |
| format | Article |
| id | mit-1721.1/101197 |
| institution | Massachusetts Institute of Technology |
| language | en_US |
| last_indexed | 2024-09-23T09:04:44Z |
| publishDate | 2016 |
| publisher | American Chemical Society (ACS) |
| record_format | dspace |
| spelling | mit-1721.1/1011972022-09-26T10:18:52Z Clotting Mimicry from Robust Hemostatic Bandages Based on Self-Assembling Peptides Tschabrunn, Cory M. Mehta, Manav Perez-Cuevas, Monica B. Zhang, Shuguang Hsu, Bryan Boen Conway, William E. Hammond, Paula T Massachusetts Institute of Technology. Institute for Soldier Nanotechnologies Massachusetts Institute of Technology. Center for Bits and Atoms Massachusetts Institute of Technology. Department of Architecture Massachusetts Institute of Technology. Department of Chemical Engineering Massachusetts Institute of Technology. Department of Chemistry Massachusetts Institute of Technology. Department of Physics Massachusetts Institute of Technology. Media Laboratory Koch Institute for Integrative Cancer Research at MIT Program in Media Arts and Sciences (Massachusetts Institute of Technology) Hsu, Bryan Boen Conway, William E. Zhang, Shuguang Hammond, Paula T. Uncontrolled bleeding from traumatic wounds is a major factor in deaths resulting from military conflict, accidents, disasters and crime. Self-assembling peptide nanofibers have shown superior hemostatic activity, and herein, we elucidate their mechanism by visualizing the formation of nanofiber-based clots that aggregate blood components with a similar morphology to fibrin-based clots. Furthermore, to enhance its direct application to a wound, we developed layer-by-layer assembled thin film coatings onto common materials used for wound dressings—gauze and gelatin sponges. We find these nanofibers elute upon hydration under physiological conditions and generate nanofiber-based clots with blood. After exposure to a range of harsh temperature conditions (−80 to 60 °C) for a week and even 5 months at 60 °C, these hemostatic bandages remain capable of releasing active nanofibers. In addition, the application of these nanofiber-based films from gauze bandages was found to accelerate hemostasis in porcine skin wounds as compared to plain gauze. The thermal robustness, in combination with the self-assembling peptide’s potent hemostatic activity, biocompatibility, biodegradability, and low cost of production, makes this a promising approach for a cheap yet effective hemostatic bandage. United States. Army Research Office (Contract W911NF-13-D-0001) United States. Air Force (Contract W911NF-07-D-0004) 2016-02-17T15:33:09Z 2016-02-17T15:33:09Z 2015-08 2015-04 Article http://purl.org/eprint/type/JournalArticle 1936-0851 1936-086X http://hdl.handle.net/1721.1/101197 Hsu, Bryan B., William Conway, Cory M. Tschabrunn, Manav Mehta, Monica B. Perez-Cuevas, Shuguang Zhang, and Paula T. Hammond. “Clotting Mimicry from Robust Hemostatic Bandages Based on Self-Assembling Peptides.” ACS Nano 9, no. 9 (September 22, 2015): 9394–9406. © 2015 American Chemical Society en_US http://dx.doi.org/10.1021/acsnano.5b02374 ACS Nano Article is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use. application/pdf American Chemical Society (ACS) ACS |
| spellingShingle | Tschabrunn, Cory M. Mehta, Manav Perez-Cuevas, Monica B. Zhang, Shuguang Hsu, Bryan Boen Conway, William E. Hammond, Paula T Clotting Mimicry from Robust Hemostatic Bandages Based on Self-Assembling Peptides |
| title | Clotting Mimicry from Robust Hemostatic Bandages Based on Self-Assembling Peptides |
| title_full | Clotting Mimicry from Robust Hemostatic Bandages Based on Self-Assembling Peptides |
| title_fullStr | Clotting Mimicry from Robust Hemostatic Bandages Based on Self-Assembling Peptides |
| title_full_unstemmed | Clotting Mimicry from Robust Hemostatic Bandages Based on Self-Assembling Peptides |
| title_short | Clotting Mimicry from Robust Hemostatic Bandages Based on Self-Assembling Peptides |
| title_sort | clotting mimicry from robust hemostatic bandages based on self assembling peptides |
| url | http://hdl.handle.net/1721.1/101197 |
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