Rate-limiting step analysis of the microbial desulfurization of dibenzothiophene in a model oil system
A mechanistic analysis of the various mass transport and kinetic steps in the microbial desulfurization of dibenzothiophene (DBT) by Rhodococcus erythropolis IGTS8 in a model biphasic (oil–water), small-scale system was performed. The biocatalyst was distributed into three populations, free cells in...
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| Format: | Article |
| Language: | en_US |
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Wiley Blackwell
2016
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| Online Access: | http://hdl.handle.net/1721.1/101229 https://orcid.org/0000-0002-2046-2726 https://orcid.org/0000-0003-0437-3157 |
| _version_ | 1826196140655116288 |
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| author | Abin-Fuentes, Andres Mohamed, Magdy El-Said Leung, James Wang, Daniel I. Prather, Kristala L. Jones |
| author2 | Massachusetts Institute of Technology. Center for Biomedical Innovation |
| author_facet | Massachusetts Institute of Technology. Center for Biomedical Innovation Abin-Fuentes, Andres Mohamed, Magdy El-Said Leung, James Wang, Daniel I. Prather, Kristala L. Jones |
| author_sort | Abin-Fuentes, Andres |
| collection | MIT |
| description | A mechanistic analysis of the various mass transport and kinetic steps in the microbial desulfurization of dibenzothiophene (DBT) by Rhodococcus erythropolis IGTS8 in a model biphasic (oil–water), small-scale system was performed. The biocatalyst was distributed into three populations, free cells in the aqueous phase, cell aggregates and oil–adhered cells, and the fraction of cells in each population was measured. The power input per volume (P/V) and the impeller tip speed (v[subscript tip]) were identified as key operating parameters in determining whether the system is mass transport controlled or kinetically controlled. Oil–water DBT mass transport was found to not be limiting under the conditions tested. Experimental results at both the 100 mL and 4 L (bioreactor) scales suggest that agitation leading to P/V greater than 10,000 W/ m3 and/or v[subscript tip] greater than 0.67 m/s is sufficient to overcome the major mass transport limitation in the system, which was the diffusion of DBT within the biocatalyst aggregates. |
| first_indexed | 2024-09-23T10:22:10Z |
| format | Article |
| id | mit-1721.1/101229 |
| institution | Massachusetts Institute of Technology |
| language | en_US |
| last_indexed | 2024-09-23T10:22:10Z |
| publishDate | 2016 |
| publisher | Wiley Blackwell |
| record_format | dspace |
| spelling | mit-1721.1/1012292022-09-26T17:28:15Z Rate-limiting step analysis of the microbial desulfurization of dibenzothiophene in a model oil system Abin-Fuentes, Andres Mohamed, Magdy El-Said Leung, James Wang, Daniel I. Prather, Kristala L. Jones Massachusetts Institute of Technology. Center for Biomedical Innovation Massachusetts Institute of Technology. Department of Chemical Engineering Abin-Fuentes, Andres Leung, James Wang, Daniel I. Prather, Kristala L. Jones A mechanistic analysis of the various mass transport and kinetic steps in the microbial desulfurization of dibenzothiophene (DBT) by Rhodococcus erythropolis IGTS8 in a model biphasic (oil–water), small-scale system was performed. The biocatalyst was distributed into three populations, free cells in the aqueous phase, cell aggregates and oil–adhered cells, and the fraction of cells in each population was measured. The power input per volume (P/V) and the impeller tip speed (v[subscript tip]) were identified as key operating parameters in determining whether the system is mass transport controlled or kinetically controlled. Oil–water DBT mass transport was found to not be limiting under the conditions tested. Experimental results at both the 100 mL and 4 L (bioreactor) scales suggest that agitation leading to P/V greater than 10,000 W/ m3 and/or v[subscript tip] greater than 0.67 m/s is sufficient to overcome the major mass transport limitation in the system, which was the diffusion of DBT within the biocatalyst aggregates. National Institutes of Health (U.S.). Biotechnology Training Program (Grant T32GM008334) Saudi Aramco 2016-02-22T15:28:43Z 2016-02-22T15:28:43Z 2013-11 2013-10 Article http://purl.org/eprint/type/JournalArticle 00063592 http://hdl.handle.net/1721.1/101229 Abin-Fuentes, Andres, James C. Leung, Magdy El-Said Mohamed, Daniel I.C. Wang, and Kristala L.J. Prather. “Rate-Limiting Step Analysis of the Microbial Desulfurization of Dibenzothiophene in a Model Oil System.” Biotechnology and Bioengineering 111, no. 5 (November 27, 2013): 876–884. https://orcid.org/0000-0002-2046-2726 https://orcid.org/0000-0003-0437-3157 en_US http://dx.doi.org/10.1002/bit.25148 Biotechnology and Bioengineering Creative Commons Attribution-Noncommercial-Share Alike http://creativecommons.org/licenses/by-nc-sa/4.0/ application/pdf Wiley Blackwell PMC |
| spellingShingle | Abin-Fuentes, Andres Mohamed, Magdy El-Said Leung, James Wang, Daniel I. Prather, Kristala L. Jones Rate-limiting step analysis of the microbial desulfurization of dibenzothiophene in a model oil system |
| title | Rate-limiting step analysis of the microbial desulfurization of dibenzothiophene in a model oil system |
| title_full | Rate-limiting step analysis of the microbial desulfurization of dibenzothiophene in a model oil system |
| title_fullStr | Rate-limiting step analysis of the microbial desulfurization of dibenzothiophene in a model oil system |
| title_full_unstemmed | Rate-limiting step analysis of the microbial desulfurization of dibenzothiophene in a model oil system |
| title_short | Rate-limiting step analysis of the microbial desulfurization of dibenzothiophene in a model oil system |
| title_sort | rate limiting step analysis of the microbial desulfurization of dibenzothiophene in a model oil system |
| url | http://hdl.handle.net/1721.1/101229 https://orcid.org/0000-0002-2046-2726 https://orcid.org/0000-0003-0437-3157 |
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