Review of methods to probe single cell metabolism and bioenergetics
Single cell investigations have enabled unexpected discoveries, such as the existence of biological noise and phenotypic switching in infection, metabolism and treatment. Herein, we review methods that enable such single cell investigations specific to metabolism and bioenergetics. Firstly, we discu...
| Main Authors: | , |
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| Other Authors: | |
| Format: | Article |
| Language: | en_US |
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Elsevier
2016
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| Online Access: | http://hdl.handle.net/1721.1/101247 https://orcid.org/0000-0001-6909-4568 |
| _version_ | 1811090302972198912 |
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| author | Vasdekis, Andreas E. Stephanopoulos, Gregory |
| author2 | Massachusetts Institute of Technology. Department of Chemical Engineering |
| author_facet | Massachusetts Institute of Technology. Department of Chemical Engineering Vasdekis, Andreas E. Stephanopoulos, Gregory |
| author_sort | Vasdekis, Andreas E. |
| collection | MIT |
| description | Single cell investigations have enabled unexpected discoveries, such as the existence of biological noise and phenotypic switching in infection, metabolism and treatment. Herein, we review methods that enable such single cell investigations specific to metabolism and bioenergetics. Firstly, we discuss how to isolate and immobilize individuals from a cell suspension, including both permanent and reversible approaches. We also highlight specific advances in microbiology for its implications in metabolic engineering. Methods for probing single cell physiology and metabolism are subsequently reviewed. The primary focus therein is on dynamic and high-content profiling strategies based on label-free and fluorescence microspectroscopy and microscopy. Non-dynamic approaches, such as mass spectrometry and nuclear magnetic resonance, are also briefly discussed. |
| first_indexed | 2024-09-23T14:41:51Z |
| format | Article |
| id | mit-1721.1/101247 |
| institution | Massachusetts Institute of Technology |
| language | en_US |
| last_indexed | 2024-09-23T14:41:51Z |
| publishDate | 2016 |
| publisher | Elsevier |
| record_format | dspace |
| spelling | mit-1721.1/1012472022-09-29T10:11:02Z Review of methods to probe single cell metabolism and bioenergetics Vasdekis, Andreas E. Stephanopoulos, Gregory Massachusetts Institute of Technology. Department of Chemical Engineering Stephanopoulos, Gregory Single cell investigations have enabled unexpected discoveries, such as the existence of biological noise and phenotypic switching in infection, metabolism and treatment. Herein, we review methods that enable such single cell investigations specific to metabolism and bioenergetics. Firstly, we discuss how to isolate and immobilize individuals from a cell suspension, including both permanent and reversible approaches. We also highlight specific advances in microbiology for its implications in metabolic engineering. Methods for probing single cell physiology and metabolism are subsequently reviewed. The primary focus therein is on dynamic and high-content profiling strategies based on label-free and fluorescence microspectroscopy and microscopy. Non-dynamic approaches, such as mass spectrometry and nuclear magnetic resonance, are also briefly discussed. United States. Dept. of Energy. Genomic Sciences Program (Grant DE-SC0008744) 2016-02-23T20:30:05Z 2016-02-23T20:30:05Z 2014-10 2014-09 Article http://purl.org/eprint/type/JournalArticle 10967176 1096-7184 http://hdl.handle.net/1721.1/101247 Vasdekis, Andreas E., and Gregory Stephanopoulos. “Review of Methods to Probe Single Cell Metabolism and Bioenergetics.” Metabolic Engineering 27 (January 2015): 115–135. https://orcid.org/0000-0001-6909-4568 en_US http://dx.doi.org/10.1016/j.ymben.2014.09.007 Metabolic Engineering Creative Commons Attribution-NonCommercial-NoDerivs License http://creativecommons.org/licenses/by-nc-nd/4.0/ application/pdf Elsevier PMC |
| spellingShingle | Vasdekis, Andreas E. Stephanopoulos, Gregory Review of methods to probe single cell metabolism and bioenergetics |
| title | Review of methods to probe single cell metabolism and bioenergetics |
| title_full | Review of methods to probe single cell metabolism and bioenergetics |
| title_fullStr | Review of methods to probe single cell metabolism and bioenergetics |
| title_full_unstemmed | Review of methods to probe single cell metabolism and bioenergetics |
| title_short | Review of methods to probe single cell metabolism and bioenergetics |
| title_sort | review of methods to probe single cell metabolism and bioenergetics |
| url | http://hdl.handle.net/1721.1/101247 https://orcid.org/0000-0001-6909-4568 |
| work_keys_str_mv | AT vasdekisandrease reviewofmethodstoprobesinglecellmetabolismandbioenergetics AT stephanopoulosgregory reviewofmethodstoprobesinglecellmetabolismandbioenergetics |