Optical measurement of biomechanical properties of individual erythrocytes from a sickle cell patient
Sickle cell disease (SCD) is characterized by the abnormal deformation of red blood cells (RBCs) in the deoxygenated condition, as their elongated shape leads to compromised circulation. The pathophysiology of SCD is influenced by both the biomechanical properties of RBCs and their hemodynamic prope...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | en_US |
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Elsevier
2016
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| Online Access: | http://hdl.handle.net/1721.1/101250 https://orcid.org/0000-0002-6223-6831 |
| _version_ | 1811096360125988864 |
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| author | Byun, HeeSu Hillman, Timothy R. Higgins, John M. Diez-Silva, Monica Peng, Zhangli Dao, Ming Suresh, Subra Park, YongKeun Dasari, Ramachandra Rao |
| author2 | Massachusetts Institute of Technology. Department of Chemistry |
| author_facet | Massachusetts Institute of Technology. Department of Chemistry Byun, HeeSu Hillman, Timothy R. Higgins, John M. Diez-Silva, Monica Peng, Zhangli Dao, Ming Suresh, Subra Park, YongKeun Dasari, Ramachandra Rao |
| author_sort | Byun, HeeSu |
| collection | MIT |
| description | Sickle cell disease (SCD) is characterized by the abnormal deformation of red blood cells (RBCs) in the deoxygenated condition, as their elongated shape leads to compromised circulation. The pathophysiology of SCD is influenced by both the biomechanical properties of RBCs and their hemodynamic properties in the microvasculature. A major challenge in the study of SCD involves accurate characterization of the biomechanical properties of individual RBCs with minimum sample perturbation. Here we report the biomechanical properties of individual RBCs from a SCD patient using a non-invasive laser interferometric technique. We optically measure the dynamic membrane fluctuations of RBCs. The measurements are analyzed with a previously validated membrane model to retrieve key mechanical properties of the cells: bending modulus; shear modulus; area expansion modulus; and cytoplasmic viscosity. We find that high cytoplasmic viscosity at ambient oxygen concentration is principally responsible for the significantly decreased dynamic membrane fluctuations in RBCs with SCD, and that the mechanical properties of the membrane cortex of irreversibly sickled cells (ISCs) are different from those of the other types of RBCs in SCD. |
| first_indexed | 2024-09-23T16:42:36Z |
| format | Article |
| id | mit-1721.1/101250 |
| institution | Massachusetts Institute of Technology |
| language | en_US |
| last_indexed | 2024-09-23T16:42:36Z |
| publishDate | 2016 |
| publisher | Elsevier |
| record_format | dspace |
| spelling | mit-1721.1/1012502022-09-29T20:58:07Z Optical measurement of biomechanical properties of individual erythrocytes from a sickle cell patient Byun, HeeSu Hillman, Timothy R. Higgins, John M. Diez-Silva, Monica Peng, Zhangli Dao, Ming Suresh, Subra Park, YongKeun Dasari, Ramachandra Rao Massachusetts Institute of Technology. Department of Chemistry Massachusetts Institute of Technology. Department of Materials Science and Engineering Massachusetts Institute of Technology. Spectroscopy Laboratory Hillman, Timothy R. Diez-Silva, Monica Peng, Zhangli Dao, Ming Dasari, Ramachandra Rao Suresh, Subra Sickle cell disease (SCD) is characterized by the abnormal deformation of red blood cells (RBCs) in the deoxygenated condition, as their elongated shape leads to compromised circulation. The pathophysiology of SCD is influenced by both the biomechanical properties of RBCs and their hemodynamic properties in the microvasculature. A major challenge in the study of SCD involves accurate characterization of the biomechanical properties of individual RBCs with minimum sample perturbation. Here we report the biomechanical properties of individual RBCs from a SCD patient using a non-invasive laser interferometric technique. We optically measure the dynamic membrane fluctuations of RBCs. The measurements are analyzed with a previously validated membrane model to retrieve key mechanical properties of the cells: bending modulus; shear modulus; area expansion modulus; and cytoplasmic viscosity. We find that high cytoplasmic viscosity at ambient oxygen concentration is principally responsible for the significantly decreased dynamic membrane fluctuations in RBCs with SCD, and that the mechanical properties of the membrane cortex of irreversibly sickled cells (ISCs) are different from those of the other types of RBCs in SCD. National Institutes of Health (U.S.) (Grant 9P41-EB015871-26A1) National Institutes of Health (U.S.) (Grant R01HL094270) National Institutes of Health (U.S.) (Grant DK083242) Korea Advanced Institute of Science and Technology Korea Advanced Institute of Science and Technology. Institute for Optical Science and Technology Korea (South). Ministry of Education, Science and Technology (MEST) (Grant 2009-0087691) National Research Foundation of Korea (NRF-2012R1A1A1009082) Singapore-MIT Alliance for Research and Technology 2016-02-24T15:34:42Z 2016-02-24T15:34:42Z 2012-07 2012-06 Article http://purl.org/eprint/type/JournalArticle 17427061 http://hdl.handle.net/1721.1/101250 Byun, HeeSu, Timothy R. Hillman, John M. Higgins, Monica Diez-Silva, Zhangli Peng, Ming Dao, Ramachandra R. Dasari, Subra Suresh, and YongKeun Park. “Optical Measurement of Biomechanical Properties of Individual Erythrocytes from a Sickle Cell Patient.” Acta Biomaterialia 8, no. 11 (November 2012): 4130–4138. https://orcid.org/0000-0002-6223-6831 en_US http://dx.doi.org/10.1016/j.actbio.2012.07.011 Acta Biomaterialia Creative Commons Attribution-Noncommercial-NoDerivatives http://creativecommons.org/licenses/by-nc-nd/4.0/ application/pdf Elsevier PMC |
| spellingShingle | Byun, HeeSu Hillman, Timothy R. Higgins, John M. Diez-Silva, Monica Peng, Zhangli Dao, Ming Suresh, Subra Park, YongKeun Dasari, Ramachandra Rao Optical measurement of biomechanical properties of individual erythrocytes from a sickle cell patient |
| title | Optical measurement of biomechanical properties of individual erythrocytes from a sickle cell patient |
| title_full | Optical measurement of biomechanical properties of individual erythrocytes from a sickle cell patient |
| title_fullStr | Optical measurement of biomechanical properties of individual erythrocytes from a sickle cell patient |
| title_full_unstemmed | Optical measurement of biomechanical properties of individual erythrocytes from a sickle cell patient |
| title_short | Optical measurement of biomechanical properties of individual erythrocytes from a sickle cell patient |
| title_sort | optical measurement of biomechanical properties of individual erythrocytes from a sickle cell patient |
| url | http://hdl.handle.net/1721.1/101250 https://orcid.org/0000-0002-6223-6831 |
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