Spatiotemporal Intracellular Nitric Oxide Signaling Captured Using Internalized, Near-Infrared Fluorescent Carbon Nanotube Nanosensors

Spatiotemporal Intracellular Nitric Oxide Signaling Captured Using Internalized, Near-Infrared Fluorescent Carbon Nanotube Nanosensors

Fluorescent nanosensor probes have suffered from limited molecular recognition and a dearth of strategies for spatial-temporal operation in cell culture. In this work, we spatially imaged the dynamics of nitric oxide (NO) signaling, important in numerous pathologies and physiological functions, usin...

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Manylion Llyfryddiaeth
Prif Awduron: Ulissi, Zachary W., Sen, Fatih, Gong, Xun, Sen, Selda, Iverson, Nicole, Boghossian, Ardemis A., Godoy, Luiz C., Wogan, Gerald N., Mukhopadhyay, Debabrata, Strano, Michael S., Selda, Sen
Awduron Eraill: Massachusetts Institute of Technology. Department of Biological Engineering
Fformat: Erthygl
Iaith:en_US
Cyhoeddwyd: American Chemical Society (ACS) 2016
Mynediad Ar-lein:http://hdl.handle.net/1721.1/101259
https://orcid.org/0000-0003-0771-9889
https://orcid.org/0000-0003-2944-808X
https://orcid.org/0000-0002-5166-1410
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Crynodeb:Fluorescent nanosensor probes have suffered from limited molecular recognition and a dearth of strategies for spatial-temporal operation in cell culture. In this work, we spatially imaged the dynamics of nitric oxide (NO) signaling, important in numerous pathologies and physiological functions, using intracellular near-infrared fluorescent single-walled carbon nanotubes. The observed spatial-temporal NO signaling gradients clarify and refine the existing paradigm of NO signaling based on averaged local concentrations. This work enables the study of transient intracellular phenomena associated with signaling and therapeutics.

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