Spatiotemporal Intracellular Nitric Oxide Signaling Captured Using Internalized, Near-Infrared Fluorescent Carbon Nanotube Nanosensors
Fluorescent nanosensor probes have suffered from limited molecular recognition and a dearth of strategies for spatial-temporal operation in cell culture. In this work, we spatially imaged the dynamics of nitric oxide (NO) signaling, important in numerous pathologies and physiological functions, usin...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | en_US |
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American Chemical Society (ACS)
2016
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| Online Access: | http://hdl.handle.net/1721.1/101259 https://orcid.org/0000-0003-0771-9889 https://orcid.org/0000-0003-2944-808X https://orcid.org/0000-0002-5166-1410 |
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| author | Ulissi, Zachary W. Sen, Fatih Gong, Xun Sen, Selda Iverson, Nicole Boghossian, Ardemis A. Godoy, Luiz C. Wogan, Gerald N. Mukhopadhyay, Debabrata Strano, Michael S. Selda, Sen |
| author2 | Massachusetts Institute of Technology. Department of Biological Engineering |
| author_facet | Massachusetts Institute of Technology. Department of Biological Engineering Ulissi, Zachary W. Sen, Fatih Gong, Xun Sen, Selda Iverson, Nicole Boghossian, Ardemis A. Godoy, Luiz C. Wogan, Gerald N. Mukhopadhyay, Debabrata Strano, Michael S. Selda, Sen |
| author_sort | Ulissi, Zachary W. |
| collection | MIT |
| description | Fluorescent nanosensor probes have suffered from limited molecular recognition and a dearth of strategies for spatial-temporal operation in cell culture. In this work, we spatially imaged the dynamics of nitric oxide (NO) signaling, important in numerous pathologies and physiological functions, using intracellular near-infrared fluorescent single-walled carbon nanotubes. The observed spatial-temporal NO signaling gradients clarify and refine the existing paradigm of NO signaling based on averaged local concentrations. This work enables the study of transient intracellular phenomena associated with signaling and therapeutics. |
| first_indexed | 2024-09-23T12:16:05Z |
| format | Article |
| id | mit-1721.1/101259 |
| institution | Massachusetts Institute of Technology |
| language | en_US |
| last_indexed | 2024-09-23T12:16:05Z |
| publishDate | 2016 |
| publisher | American Chemical Society (ACS) |
| record_format | dspace |
| spelling | mit-1721.1/1012592022-09-28T00:52:11Z Spatiotemporal Intracellular Nitric Oxide Signaling Captured Using Internalized, Near-Infrared Fluorescent Carbon Nanotube Nanosensors Ulissi, Zachary W. Sen, Fatih Gong, Xun Sen, Selda Iverson, Nicole Boghossian, Ardemis A. Godoy, Luiz C. Wogan, Gerald N. Mukhopadhyay, Debabrata Strano, Michael S. Selda, Sen Massachusetts Institute of Technology. Department of Biological Engineering Massachusetts Institute of Technology. Department of Chemical Engineering Ulissi, Zachary W. Sen, Fatih Selda, Sen Iverson, Nicole Boghossian, Ardemis A. Godoy, Luiz C. Wogan, Gerald N. Strano, Michael S. Fluorescent nanosensor probes have suffered from limited molecular recognition and a dearth of strategies for spatial-temporal operation in cell culture. In this work, we spatially imaged the dynamics of nitric oxide (NO) signaling, important in numerous pathologies and physiological functions, using intracellular near-infrared fluorescent single-walled carbon nanotubes. The observed spatial-temporal NO signaling gradients clarify and refine the existing paradigm of NO signaling based on averaged local concentrations. This work enables the study of transient intracellular phenomena associated with signaling and therapeutics. National Science Foundation (U.S.) United States. Dept. of Energy. Computational Science Graduate Fellowship Program (Grant DE-FG02-97ER25308) United States. Dept. of Defense (National Defense Science and Engineering Graduate (NDSEG) Fellowship) 2016-02-24T18:49:58Z 2016-02-24T18:49:58Z 2014-07 2014-06 Article http://purl.org/eprint/type/JournalArticle 1530-6984 1530-6992 http://hdl.handle.net/1721.1/101259 Ulissi, Zachary W., Fatih Sen, Xun Gong, Selda Sen, Nicole Iverson, Ardemis A. Boghossian, Luiz C. Godoy, Gerald N. Wogan, Debabrata Mukhopadhyay, and Michael S. Strano. “Spatiotemporal Intracellular Nitric Oxide Signaling Captured Using Internalized, Near-Infrared Fluorescent Carbon Nanotube Nanosensors.” Nano Lett. 14, no. 8 (August 13, 2014): 4887–4894. © 2014 American Chemical Society https://orcid.org/0000-0003-0771-9889 https://orcid.org/0000-0003-2944-808X https://orcid.org/0000-0002-5166-1410 en_US http://dx.doi.org/10.1021/nl502338y Nano Letters Article is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use. application/pdf American Chemical Society (ACS) ACS |
| spellingShingle | Ulissi, Zachary W. Sen, Fatih Gong, Xun Sen, Selda Iverson, Nicole Boghossian, Ardemis A. Godoy, Luiz C. Wogan, Gerald N. Mukhopadhyay, Debabrata Strano, Michael S. Selda, Sen Spatiotemporal Intracellular Nitric Oxide Signaling Captured Using Internalized, Near-Infrared Fluorescent Carbon Nanotube Nanosensors |
| title | Spatiotemporal Intracellular Nitric Oxide Signaling Captured Using Internalized, Near-Infrared Fluorescent Carbon Nanotube Nanosensors |
| title_full | Spatiotemporal Intracellular Nitric Oxide Signaling Captured Using Internalized, Near-Infrared Fluorescent Carbon Nanotube Nanosensors |
| title_fullStr | Spatiotemporal Intracellular Nitric Oxide Signaling Captured Using Internalized, Near-Infrared Fluorescent Carbon Nanotube Nanosensors |
| title_full_unstemmed | Spatiotemporal Intracellular Nitric Oxide Signaling Captured Using Internalized, Near-Infrared Fluorescent Carbon Nanotube Nanosensors |
| title_short | Spatiotemporal Intracellular Nitric Oxide Signaling Captured Using Internalized, Near-Infrared Fluorescent Carbon Nanotube Nanosensors |
| title_sort | spatiotemporal intracellular nitric oxide signaling captured using internalized near infrared fluorescent carbon nanotube nanosensors |
| url | http://hdl.handle.net/1721.1/101259 https://orcid.org/0000-0003-0771-9889 https://orcid.org/0000-0003-2944-808X https://orcid.org/0000-0002-5166-1410 |
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