Condensin promotes the juxtaposition of DNA flanking its loading site in Bacillus Subtilis
SMC condensin complexes play a central role in compacting and resolving replicated chromosomes in virtually all organisms, yet how they accomplish this remains elusive. In Bacillus subtilis, condensin is loaded at centromeric parS sites, where it encircles DNA and individualizes newly replicated ori...
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| Format: | Article |
| Language: | en_US |
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Cold Spring Harbor Laboratory Press
2016
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| Online Access: | http://hdl.handle.net/1721.1/101399 https://orcid.org/0000-0003-4764-8851 https://orcid.org/0000-0002-8288-7607 |
| _version_ | 1811092371756023808 |
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| author | Wang, Xindan Lajoie, Bryan R. Dekker, Job Rudner, David Z. Laub, Michael T Le, Tung |
| author2 | Massachusetts Institute of Technology. Department of Biology |
| author_facet | Massachusetts Institute of Technology. Department of Biology Wang, Xindan Lajoie, Bryan R. Dekker, Job Rudner, David Z. Laub, Michael T Le, Tung |
| author_sort | Wang, Xindan |
| collection | MIT |
| description | SMC condensin complexes play a central role in compacting and resolving replicated chromosomes in virtually all organisms, yet how they accomplish this remains elusive. In Bacillus subtilis, condensin is loaded at centromeric parS sites, where it encircles DNA and individualizes newly replicated origins. Using chromosome conformation capture and cytological assays, we show that condensin recruitment to origin-proximal parS sites is required for the juxtaposition of the two chromosome arms. Recruitment to ectopic parS sites promotes alignment of large tracks of DNA flanking these sites. Importantly, insertion of parS sites on opposing arms indicates that these “zip-up” interactions only occur between adjacent DNA segments. Collectively, our data suggest that condensin resolves replicated origins by promoting the juxtaposition of DNA flanking parS sites, drawing sister origins in on themselves and away from each other. These results are consistent with a model in which condensin encircles the DNA flanking its loading site and then slides down, tethering the two arms together. Lengthwise condensation via loop extrusion could provide a generalizable mechanism by which condensin complexes act dynamically to individualize origins in B. subtilis and, when loaded along eukaryotic chromosomes, resolve them during mitosis. |
| first_indexed | 2024-09-23T15:17:06Z |
| format | Article |
| id | mit-1721.1/101399 |
| institution | Massachusetts Institute of Technology |
| language | en_US |
| last_indexed | 2024-09-23T15:17:06Z |
| publishDate | 2016 |
| publisher | Cold Spring Harbor Laboratory Press |
| record_format | dspace |
| spelling | mit-1721.1/1013992022-09-29T13:55:28Z Condensin promotes the juxtaposition of DNA flanking its loading site in Bacillus Subtilis Wang, Xindan Lajoie, Bryan R. Dekker, Job Rudner, David Z. Laub, Michael T Le, Tung Massachusetts Institute of Technology. Department of Biology Le, Tung B. K. Laub, Michael T. SMC condensin complexes play a central role in compacting and resolving replicated chromosomes in virtually all organisms, yet how they accomplish this remains elusive. In Bacillus subtilis, condensin is loaded at centromeric parS sites, where it encircles DNA and individualizes newly replicated origins. Using chromosome conformation capture and cytological assays, we show that condensin recruitment to origin-proximal parS sites is required for the juxtaposition of the two chromosome arms. Recruitment to ectopic parS sites promotes alignment of large tracks of DNA flanking these sites. Importantly, insertion of parS sites on opposing arms indicates that these “zip-up” interactions only occur between adjacent DNA segments. Collectively, our data suggest that condensin resolves replicated origins by promoting the juxtaposition of DNA flanking parS sites, drawing sister origins in on themselves and away from each other. These results are consistent with a model in which condensin encircles the DNA flanking its loading site and then slides down, tethering the two arms together. Lengthwise condensation via loop extrusion could provide a generalizable mechanism by which condensin complexes act dynamically to individualize origins in B. subtilis and, when loaded along eukaryotic chromosomes, resolve them during mitosis. National Institutes of Health (U.S.) (Grant GM082899) 2016-03-02T02:57:58Z 2016-03-02T02:57:58Z 2015-08 2015-05 Article http://purl.org/eprint/type/JournalArticle 0890-9369 1549-5477 http://hdl.handle.net/1721.1/101399 Wang, Xindan, Tung B.K. Le, Bryan R. Lajoie, Job Dekker, Michael T. Laub, and David Z. Rudner. “Condensin Promotes the Juxtaposition of DNA Flanking Its Loading Site in Bacillus Subtilis.” Genes Dev. 29, no. 15 (August 1, 2015): 1661–1675. https://orcid.org/0000-0003-4764-8851 https://orcid.org/0000-0002-8288-7607 en_US http://dx.doi.org/10.1101/gad.265876.115 Genes & Development Creative Commons Attribution http://creativecommons.org/licenses/by-nc/4.0/ application/pdf Cold Spring Harbor Laboratory Press Cold Spring Harbor Laboratory Press |
| spellingShingle | Wang, Xindan Lajoie, Bryan R. Dekker, Job Rudner, David Z. Laub, Michael T Le, Tung Condensin promotes the juxtaposition of DNA flanking its loading site in Bacillus Subtilis |
| title | Condensin promotes the juxtaposition of DNA flanking its loading site in Bacillus Subtilis |
| title_full | Condensin promotes the juxtaposition of DNA flanking its loading site in Bacillus Subtilis |
| title_fullStr | Condensin promotes the juxtaposition of DNA flanking its loading site in Bacillus Subtilis |
| title_full_unstemmed | Condensin promotes the juxtaposition of DNA flanking its loading site in Bacillus Subtilis |
| title_short | Condensin promotes the juxtaposition of DNA flanking its loading site in Bacillus Subtilis |
| title_sort | condensin promotes the juxtaposition of dna flanking its loading site in bacillus subtilis |
| url | http://hdl.handle.net/1721.1/101399 https://orcid.org/0000-0003-4764-8851 https://orcid.org/0000-0002-8288-7607 |
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